The Effect of Different Running Treadmill Exercises on Oxidative Stress Parameters in Rats Muscle Tissue

Abstract

Aim of this study was planned to determine the effect of treadmill exercises made on different occasions on the oxidative stress parameters on the rat muscle tissue. In the present study, 32 Male Albino Wistar rats weighing 280-350 grams were used. Rats were administered a treadmill exercise protocol for 13 weeks, short (15 minutes, n:8), modarate (30 minutes, n:8) and long time (60 minutes, n:8) for five days. For each biochemical study, superoxide dismutase activity (SOD), malondialdehyde (MDA) levels and glutathione (GSH) levels from each supernatant were measured in duplicate in each rat muscle tissue by ELISA kits, respectively. Results from the experiments were given as mean±standard deviation, and P values below 0.05 were considered statistically significant. The significance level difference between groups was determined using the one-way analysis of variance (ANOVA) test and post-hoctests using the "LSD" technique. The control group consisted of 23,07 ± 3,38 (U/mg protein), 15 min group (19,82 ± 4,77 U/mg protein) and 30,88 ± 4,18 (U/Running was determined as SOD value of 60 min 22.15 ± 3.85 (U/mg protein). The control group consisted of 0,43 ± 0,6 (nmol/mg protein), 15 minutes group with 0,35 ± 0,3 (nmol/mg protein), 30 minutes 0,23 ± 0,6^ab (nmol/mg protein), Running 60 min 0,24 ± 0,1 (nmol / mg protein), GSH value. The control group consisted of 0.19 ± 0.02 (nmol/mg protein), 15 min group of running 0.12 ± 0.03 (nmol/mg protein), running 30 min 0.14 ± 0.01 (nmol/mg protein), Running was 0,14 ± 0,01 (nmol/mg protein), GSH valuefor 60 min. As a result, it was determined that treadmill exercise performed at different times had a positive effect on the oxidative stress parameters occurring in rat muscle. It may be suggested that the antioxidant supplementation taken with the exercises made at different times may be more effective on muscle tissue. 

Keywords

• Exercise,Antioxidant,Free Radical

Farklı sürelerdeki koşu bandı egzersizinin sıçan kas dokusundaki oksidatif stres parametreleri üzerine etkisi

Öz

Bu çalışmanın amacı; farklı sürelerde yapılan koşu bandı egzersizlerinin sıçan kas dokusu üzerinde meydana gelen oksidatif stres parametrelerine etkisini belirlemektir. Sunulan çalışmada ağırlıkları 280-350 gram olan Albino Wistar cinsi 32 adet erkek sıçan kullanılmıştır. Sıçanlara, 13 hafta boyunca koşu bandı egzersiz protokolü, kısa (15 dakika, n:8), orta (30 dakika, n:8) ve yüksek (60 dakika, n:8) düzeyde, beş gün arasında uygulanmıştır. Biyokimyasal çalışmalar için her süpernatanttan süperoksit dismutaz aktivitesi (SOD), malondialdehit (MDA) seviyeleri ve glutatyon (GSH) seviyeleri sırasıyla ELISA kitleriyle her bir rat kas dokusu ikişer tekrarlamalı olarak ölçülmüştür. Deneylerden elde edilen sonuçlar ortalama ± standart sapma olarak verildi ve 0.05'in altındaki P değerleri, istatistiksel açıdan anlamlı olarak kabul edilmiştir. Gruplar arası farkın önemlilik derecesi tek yönlü varyans analizi (ANOVA) testi ile ve post-hoc testlerinden “LSD” tekniği kullanılarak belirlenmiştir. Kontrol grubu 23,07±3,38 (U/mg protein), Koşu 15 dk. grubu 19,82±4,77 (U/mg protein), Koşu 30 dk. 20,88±4,18 (U/mg protein) Koşu 60 dk. 22,15±3,85 (U/mg protein) SOD değeri olarak tespit edilmiştir. Kontrol grubu 0,43±0,6 (nmol/mg protein), Koşu 15 dk. grubu 0,35±0,3 (nmol/mg protein), Koşu 30 dk. 0,23±0,6^ab (nmol/mg protein), Koşu 60 dk. 0,24±0,1(nmol/mg protein), GSH değeri olarak tespit edilmiştir. Kontrol grubu 0,19±0,02 (nmol/mg protein), Koşu 15 dk. grubu 0,12±0,03 (nmol/mg protein), Koşu 30 dk. 0,14±0,01 (nmol/mg protein), Koşu 60 dk. 0,14±0,01 (nmol/mg protein), GSH değeri olarak tespit edilmiştir. Sonuç olarak farklı sürelerde yapılan koşu bandı egzersizi sıçan kas dokusunda meydana gelen oksidatif stres parametreleri üzerinde pozitif etkisinin olduğu tespit edilmiştir. Farklı sürelerde yapılan egzersizler ile birlikte alınan antioksidan desteğinin kas dokusu üzerinde daha etkili olabileceği önerilebilir. 

Anahtar Kelimeler

• Egzersiz,Antioksidan,Serbest Radikal

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