Investıgatıon Of Cape's Effect On The Presence Of Matrıx Proteıns In Gastric And Colon Cancer Cell Lınes

Öz

Objective: The formation and progression of cancer depend on the abilities of body cells such as uncontrolled proliferation, migration, invasion, metastasis, angiogenesis, and escape from apoptosis, as well as its microenvironment. Therefore, in order to understand cancer cell behaviors, it is important to know the specific components that make up the cancer microenvironment and to understand the mechanisms by which these components communicate. In this study, we aimed to compare the effect of therapeutic dose of CAPE as an anticancer agent in gastric and colon cancer cell lines in terms of markers associated with angiogenesis and apoptosis in the presence of matrix proteins. Materials and Methods: After the addition of CAPE to gastric cancer cell line (NCI-N87) and colon cancer cell line (Colo 205) in the presence of matrix proteins, we measured the expressions of angiogenesis and apoptosis-related markers by Real Time PCR at 260 nm absorbance. Results: It was observed that apoptosis increased more in the presence of matrix proteins and CAPE addition in gastric cancer, and angiogenesis increased more in colon cancer. Conclusion: In our study, we observed that the laminin matrix protein was the main culprit and that angiogenesis and apoptosis were triggered more by the addition of Kafeik asit (3,4-dihidroksisinnamik asit) fenetil ester (CAPE). We plan to support this study with animal experiments to show that CAPE can be a therapeutic compound for gastric and colon cancer patients.

Anahtar Kelimeler

• Cancer cell culture
• Angiogenesis
• Apoptosis
• Matrix proteins
• CAPE

Mide ve Kolon Kanseri Hücre Hatlarında Matriks Proteinlerinin Varlığında Cape’nin Etkisinin Karşılaştırılması

Öz

Amaç: Çağımızın en önemli hastalıklarından biri olan kanser, vücut hücrelerinin kontrolsüz çoğalması ve yayılmasıyla oluşan ciddi bir sağlık sorunudur. Aynı zamanda kronik inflamasyon ve hastanın bağışıklık sisteminin zayıflaması ile karakterize ve ölüme en sık neden olan hastalıklardan biridir. Kanser hücre davranışlarını anlamak için kanser mikroçevresini oluşturan spesifik bileşenlerin bilinmesi, bu bileşenlerin hangi mekanizmaları kullanarak iletişim kurduklarının anlaşılması önemlidir.Bizde yaptığımız çalışmada bir antikanser ajan olarak Cape'nin terapötik dozunun etkisini mide ve kolon kanseri hücre hatlarında matriks proteini olan laminin ve kollajen 1 varlığında anjiogenez ve apoptoz ile ilişkili markerler açısından karşılaştırmalı değerlendirmeyi amaçladık. Gereç ve Yöntem: Mide kanseri hücre hattı NCI-N87 ve kolon kanseri hücre hattı Colo 205 kullanılarak matriks protein varlığında Kafeik asit fenetil ester (CAPE) ilavesinden sonra DNA sentezi yapıp Real Time PCR ile 260 nm absorbansda okundu. Bulgular: Mide kanserinde matriks proteinlerinin varlığında ve CAPE ilavesinde apoptozisin, Kolon kanserinde ise angiogenezisin daha fazla arttığı görüldü. Sonuç: Yaptığımız çalışmada ana sorumlunun laminin matriks proteini olduğunu ve CAPE ilavesiyle angiogenez ve apoptosizin daha fazla tetiklendiğini gözlemledik. Bu çalışmayı hayvan deneyleri ile desteklemeyi planlamaktayız. Bu çalışmadan da çıkan sonuçlara göre mide ve kolon kanseri hastaları için CAPE’nin tedavi edici bir bileşik olabileceğini düşünmekteyiz.

Anahtar Kelimeler

• Kanser hücre kültürü
• Anjiogenesis
• Apoptozis
• Matriks proteinleri
• CAPE

Kaynakça

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