Investigation of Cytotoxic Effect of Origanum minutiflorum on Cancer Cells

Yıl 2019, , 74 - 80, 28.06.2019

OKTAY Özkan , Caner Özbey Işıl Aydemir , İsmail Sari , Gonca Dönmez , Fatma Esin Kırık Ahmet Savran Mehmet İbrahim Tuğlu

https://doi.org/10.34087/cbusbed.405472

Öz

Objective: Cancer is
defined by a abnormal growth of cells, that invade tissues and organs and
spread to other parts of the body. Breast cancer is one of the most repeatedly
diagnosed cancers and the foremost cause of death among women worldwide. Medicinal
plants can have an anticancerogenic effect in some cancer types. Our aim is to
search the cytotoxic effect of Origanum minutiflorum O.
Schwarz & P.H. Davis on breast cancer cells in vitro conditions.

Methods: In this
study, MDA-MB-231 and MCF-7 breast cancer cell lines and adipose tissue derived
mesenchymal stem cells were used. Essential oil from Origanum
minutiflorum was exposed to
the all cells and IC50 dose was calculated by MTT assay. We have examined
immunocytochemical staining of eNOS, p53, PCNA and TUNEL at IC50 dose for each
cells. The results were evaluated using one-way-ANOVA by Graphpad software.

Results: The
proliferation of breast cancer cells were inhibited by oregano oil. Oregano oil
did not show any cytotoxic effect in mesenchymal stem cells. It was determined
that cytotoxic effect of oregano oil on cancer cells was occurred through
increase of eNOS and p53 staining for survival and the TUNEL labeling for
apoptosis, and decreased of PCNA staining for proliferation.

Conclusion: According
to our data, oregano oil has cytotoxic effect on MDA-MB-231 and MCF-7 breast
cancer cell lines, and it needs to be analysis to detect active components for
other biological activities.

Anahtar Kelimeler

Breast cancer; MCF-7; MDA-MB-231; Origanum minutiflorum; cytotoxicity; oxidative stress; apoptosis

Kanser hücreleri üzerine Origanum minutiflorum’un Sitotoksik Etkisinin Araştırılması

Öz

Amaç: Kanser, doku
ve organları istila eden ve vücudun diğer kısımlarına sıçrayabilen, hücrelerin
anormal gelişimidir. Meme kanseri en çok tekrarlanan tanıya sahip ve dünya
genelinde kadınlarda kanserle ilişkili ölümlerde en önde gelen sebeplerdendir.
Tıbbi bitkiler bazı kanser türlerinde antikarsinojenik özelliklere sahip
olabilmektedir. Bu çalışmanın amacı Origanum
minutiflorum O. Schwarz & P.H. Davis’in in vitro şartlarda, meme kanseri hücre dizilerinde sitotoksik
etkisini araştırmaktır.

Gereç ve
Yöntem: Bu çalışmada, MDA-MB-231 ve MCF-7 meme kanseri hücre dizileri ve adipoz
dokudan izole edilmiş mezenkimal kök hücreleri kullanıldı. Origanum minutiflorum’dan elde edilmiş esansiyel yağa maruz
bırakılmış tüm hücre dizilerinde MTT testi aracılığıyla IC50 dozu hesaplandı. Her
bir hücre dizisinin IC50 dozunda  eNOS,
p53, PCNA ve TUNEL için immunositokimya boyaması yapıldı. Sonuçlar Graphpad
yazılımı kullanılarak one-way-ANOVA kullanılarak değerlendirildi.

Bulgular: Meme
kanseri hücrelerinin proliferasyonu oragano yağı tarafından inhibe edildi.
Oregano yağı mezenkimal kök hücrelerinde herhangi bir sitotoksik etki
göstermedi. eNOS ve p53 ile apoptozisin göstergesi TUNEL’de artış yoluyla
oreagono yağının kanser hücrelerinde sitotoksik olduğu ortaya çıktı ve
PCNA boyamada proliferasyonun azaldığı tespit edildi. Sonuç: Bizim verilerimize göre oregano yağı MDA-MB-231 ve MCF-7 meme
kanseri hücrelerinde sitotoksik bir etkiye sahiptir ve diğer biyolojik
aktiviteleri için aktif komponentlerini tanımlayacak testlere ihtiyaç vardır. 

Anahtar Kelimeler

Meme kanseri, MCF-7, MDA-MB-231, Origanum minutiflorum, sitotoksisite, oksidatif stres

Kaynakça

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