Menisküs Yırtığı Hastalarının Sinoviyal Hücrelerinde Matriks Metalloprotein-2 ve NF-Kβ Protein Ekspresyonu

Abstract

Amaç: Menisküs zorlanmalara karşı emici özelliği ile eklem stabilitesini sağlar. Menisküs yırtığı ayağın hareketi sırasında ortaya çıkan ciddi bir travmadır.

Gereç ve Yöntem: Menisküs yırtığı olan 28 erkek ve 32 kadın hasta çalışmaya dahil edilmiştir. Hastaların fiziksel incelemede lokal ağrı hissi, şişme, günlük aktivitelerini yerine getirmede güçlük,  koşma ve yürüme gibi şikayetleri vardı. Hastaların manyetik rezonans görüntülemeleri değerlendirildi. Menisküs ağrısı olan dizlerin yanal suprapatellar poş kısmından enjektör ile 5 cc sinoviyal sıvıları aspire edildi. Örneklerin rutin histolojik takipleri yapıldıktan sonra parafine gömüldü ve mikrotom ile yarı-ince kesitleri alındı. İmmunohistokimyasal analiz için matriks metaloprotein (MMP-2) ve nükleer faktör kappa (NF-kβ) primer antikorları kullanıldı. Kesitlerin şık mikroskobu ile fotoğrafları çekildi ve değerlendirildi.

Bulgular: Bayan bireylerin eklem sıvılarında lenfosit ve nötrofil hücrelerinde ve hiperplastik eritrositler ile fibroblast sayısında artış gözlendi. Erkek hastalarda, fibroblast hücreleri hiperplastik olarak izlendi ve plazma hücrelerinin arttığı görüldü. MMP-2 ekspresyonunun plazma hücrelerinde, fibroblastlarda ve nötrofillerde arttığı ayrıca, NF-kβ ekspresyonunun lenfosit ve polimorfik çekirdeğe sahip hücrelerde pozitif olduğu izlendi.

Sonuçlar: Menisküs yırtığı ve enflamasyonu olan eklemlerde artmış sıvının kıkırdak matriksinde hasara neden olduğu ve buna bağlı olarak MMP-2 ve NF-kβ gibi sitokinlerin ekspresyonunun arttığı, fibroblast hücreleri gibi sentez yapan hücrelerin yıkıma uğradığı düşünülmektedir.

Keywords

• Menisküs yırtığı,Sinoviyal hücreler,MMP-2,NF-kβ

Matrix Metalloprotein-2 and NF-Kβ Protein Expressions in Synovial Cells of Meniscus Tear Patients

Abstract

Aim: Meniscus provides joint stability with its absorbent properties. Meniscal tear is a serious trauma that occurs during movement of the foot.

Materials and Methods: The present study included 28 male and 32 female patients with meniscal tears. Physical examination of the patient revealed local pain, swelling, difficulty in performing daily activities, and walking. Magnetic resonance imaging of the patients were evaluated. The synovial fluids of 5 cc were aspirated by the injector from the lateral suprapatellar pouch portion of the knees with meniscus pain. After routine histological follow-up of the samples, they were embedded in paraffin and semi-thin sections were taken with microtome. Matrix metalloprotein (MMP-2) and nuclear factor kappa (NF-kβ) primary antibodies were used for immunohistochemical analysis of the samples. Sections were photographed with light microscope and evaluated.

Results: There was an increase in the number of lymphocytes and neutrophil cells and hyperplastic erythrocytes and fibroblasts in the joint fluids of female patients. In male patients, fibroblast cells were observed to be hyperplastic and plasma cells were increased. MMP-2 expression was increased in plasma cells, fibroblasts and neutrophils besides, NF-kβ expression was positive in lymphocytes and cells with polymorphic nuclei.

Conclusion: It is believed that increased fluid in the joints with meniscal tear and inflammation caused damage in the cartilage matrix and therefore expressions of cytokines such as MMP-2 and NF-kβ were increased, and synthesizing cells such as fibroblasts were destroyed

Keywords

• Meniscus tear,Sinovial cells,MMP-2,NF-kβ,immunohistochemistry

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