Protective Effects of Vitamin E against Methotrexate-Induced Testicular Damage in Rats: Histopathologic and flow cytometric study

Abstract

Background: Methotrexate (MTX) can cause oxidative stress-related tissue damage.Vitamin E neutralizes lipid peroxidation arising from the effect of free oxygen radicals.In this study, the protective effect of vitamin E against possible MTX-related testicular damage was analyzed. Method: Thirty two mature male Spraque dawley rats were grouped as MTX, Vitamin E, MTX+Vitamin, Control groups. 20 mg/kg MTX intraperitoneal (i.p.) was applied to MTX Group in the first day; 100mg/kg i.p. vitamin E was applied to Vitamin E Group for 5 days; 20 mg/kg i.p. MTX in the first day and 100 mg/kg i.p. vitamin E for 5 days were applied to MTX+Vitamin E Group;2 ml physiological saline solution (i.p.) was applied to Control Group for 5 days. Histopathology, flow cytometry and apoptosis were evaluated on testicular tissue. Result: Apoptotic Index (%) and testicular damage were highest for MTX Group, and significant decrease was observed for MTX+Vitamin E Group compared to MTX Group.Seminiferous tubule size significant decreased in MTX Group and it increased in MTX+Vitamin E Group compared to MTX Group. No significant difference was found between MTX and MTX+Vitamin E Groups regarding germinal epithelium thickness and testicle weights. Conclusion: The results show that MTX can cause structural disruptions in testicles and vitamin E can rehabilitate MTX-related testicular damage.

Keywords

• Methotrexate,Vitamin E,Apoptosis,TUNEL,Flow cytometry

Sıçanlarda Metotreksat ile İndüklenen Testis Hasarına Karşı E Vitamininin Koruyucu Etkileri: Histopatolojik ve Akım Sitometrik Çalışma

Abstract

Amaç: Metotreksat oksidatif strese bağlı doku hasarına neden olmaktadır. E vitamini serbest oksijen radikallerinden etkisiyle oluşan lipt peroksidasyonunu nötralize eder. Bu çalışma ile E vitamininin olası Metotreksat bağlantılı testiküler hasara karşı koruyucu etkileri analiz edildi. Materyal-Metot: Otuz iki erkek Spraque dawley sıçan MTX, E Vitamini, MTX+ E Vitamini, Kontrol olarak gruplandırıldı. İlk gün MTX grubuna intraperitoneal(i.p.) 20 mg/kg MTX, E vitamini grubuna 5 gün 100 mg/kg i.p. E vitamini, MTX+E Vitamini grubuna ilk gün 20 mg/kg i.p. MTX ve 5 gün 100 mg/kg i.p. E vitamini uygulandı. Kontrol grubuna 5 gün 2 ml serum fizyolojik (i.p.) uygulandı. Testiküler dokuda histopatolojik, akım sitometrik analizler yapıldı ve apoptoz değerlendirildi. Bulgular: Apoptotik indeks (%) ve testiküler hasar en fazla MTX grubundayken, MTX+E Vitamini grubunda MTX grubuna göre anlamlı derecede azalma gözlendi. Seminifer tubule çapı MTX grubunda belirgin şekilde azalırken, MTX+E Vitamini grubunda MTX grubuna göre artış gözlendi. MTX ve MTX+E Vitamini grupları arasında germinal epitelyum ve testiküler ağırlıkta açısında anlamlı farklılık yoktu. Sonuç: Sonuçlara göre MTX testislerde yapısal hasara neden olabilirken, E vitamini MTX'e bağlı testiküler hasarı düzeltebilir.

Keywords

• Metotreksat,E vitamin,Apopitozis,TUNEL,Akım sitometri

References

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