Evaluation Of The Effects Of Different Storage Times On Pharmacological Agents Of Saffron (Crocus sativus L.) (Safranal, Crocin and Crocetin) and Their Quality Characteristics

Öz

Objective The main objective of this study is to determine how important pharmacological agents of saffron (safranal, crocin and crocetin) and its volatile components are affected at different storage times. Material and Methods: The main purpose of this study is to investigate the effects of different storage periods on the quality of basic components of saffron including safranal, crocin and crocetin using GC-MS FID analysis, which are important pharmacological agents for determining the quality of saffron. In the second study, the same amounts of samples were analyzed by GC-MS/MS analysis with the same extraction method, and the volatile components of saffron stigma were determined and the evaluation of these components was evaluated in terms of fatty acid characteristics, bioactivity and quality properties. Results: When the storage time of the stigma of the saffron plant increases, the amounts of safranal, crocin and Crocetin used as pharmacological agents decrease. During the storage period, safranal, 2,3-Dihydro-3,5-Dihydroxy-6-Methyl-4H-Pyran-4-One, Isopropylidenecyclopropyl Methyl Ketone, Ketoisophorone, Glycerol Palmitate and N-Propylacetamide amounts decrease with increasing the storage period.. the amounts of other volatile components varied with increased storage periods. When the amounts of essential fatty acid components are evaluated, the highest amount of fatty acids with 36.74% was found in the storage period of 44 months and the lowest amount was observed with 19.36% in 8 months storage. When the amounts of bioactive volatile components are evaluated, the highest amount of components with 49.17% was obtained for 8 months of storage and the lowest amount of components with 46.29% was observed for 44 months storage. , the highest amounts of total fatty acid and bioactive components with 83.03% was obtained in 44 months storage period. Conclusion: The amount of pharmacological agents (safranal, crocin, Crocetin) decreases depending on the storage time. However, fatty acid and bioactive component ratios compensate for this.

Anahtar Kelimeler

• Pharmacological agents
• saffron
• safranal
• crocin
• crocetin
• Crocus sativus L.

Farklı Depolama Sürelerinin Safranın (Crocus sativus L.) Farmakolojik Ajanlarına (Safranal, Crocin ve Crocetin) Etkisi ve Kalite Özellikleri Bakımından Değerlendirilmesi

Öz

Giriş ve Amaç: Bu çalışmanın temel amacı, safranın önemli farmakolojik ajanları (safranal, krosin ve krosetin) ve uçucu bileşenlerinin farklı depolama sürelerinde nasıl etkilendiğini belirlemektir. Gereç ve Yöntemler: Bu çalışmanın birinci aşamasında GC-MS/FID analizi ile safranın kalite kriterlerini belirlemede kullanılan önemli farmakolojik ajanlar olan safranal, crocin ve crocetin gibi temel bileşenlerin farklı depolama sürelerinin kalite üzerine etkileri araştırılmıştır. İkinci aşamada ise GC-MS/MS analizi ile stigma üzerindeki uçucu bileşenler belirlenmiş ve bu uçucu bileşenlerin karşılıklı değerlendirilmesi yapılarak, yağ asidi ve biyoaktivite özelliğine sahip uçucu bileşenlere etkileri değerlendirilmiştir. Bulgular: Safran bitkisinin stigmasının depolanma süresi uzadıkça farmakolojik ajan olarak kullanılan safranal, crocin ve Crocetin miktarları azalmaktadır. Farklı depolama sürelerinde safranal, 2,3-Dihydro-3,5-Dihydroxy-6-Methyl-4h-Pyran-4-One, Isopropylidenecyclopropyl methyl ketone, Ketoisophorone, Glycerol Palmitate ve N-Propylacetamide oranlarının depolama süreleri uzadıkça azaldıkları görülmüştür. Bunlar haricindeki diğer uçucu bileşenlerin çoğunun miktarlarında dalgalanmalar gözükmektedir. Uçucu yağ asidi bileşenlerinin oranları değerlendirildiğinde en yüksek %36.74 oranında 44 ay depolama süresinde ve en düşük %19.36 ile 8 ay depolama süresinde gerçekleşmiştir. Biyoaktif özelliğe sahip uçucu bileşen oranları değerlendirildiğinde en yüksek %49.17 ile 8 ay depolama süresinde ve en düşük ise %46.29 ile 44 ay depolama süresinde biyoaktif bileşen oranının gerçekleştiği görülmüştür. Hem yağ asidi hem de biyoaktif bileşenlere bakıldığında en yüksek oran 44 ay depolama süresinde %83.03 ile gerçekleşmiştir. Sonuç: Depolanma süresine bağlı olarak farmakolojik ajanlarının (safranal, crocin, Crocetin) miktarları azalmaktadır. Ancak yağ asidi ve biyoaktif bileşen oranları bunu telafi etmektedir.

Anahtar Kelimeler

• Farmakolojik ajanlar
• safranal
• safran
• crocin
• crocetin
• Crocus sativus L.

Kaynakça

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