Evaluation of Regeneration and Apoptosis in Experimental Hepatic Resection of Rats

Abstract

Objective: Apoptosis and accompanying regeneration begins immediately after liver resection and transpalantation. It is known that DNA synthesis reaches its maximum level in the first 24-48 hours after resection. This study was carried out to examine the regeneration and apoptotic process in the liver of rats that underwent 70% liver resection on days 3 and 7. Materials and Methods: 21 adult male Wistar Albino rats were used. The laparotomy with an upper midline incision was performed on the subjects for liver resection. Three groups were formed as the control (Group I), the group that was sacrificed 3 days after resection (Group II), and the group that was sacrificed 7 days after resection (Group III). Results: Sinusoidal dilatation and vacuolization in hepatocytes were observed in the liver tissues of all experimental groups except the control group. However, these histopathological findings were observed more in group II than other groups. It was observed that the apoptotic index and proliferation index values reached the maximum level in the group II. In group III, it was observed that these index values gradually decreased. Values of these parameters were found to be quite low in control group. Conclusion: As a result, comparisons of the mechanisms accompanying the regeneration occurring after resection were determined by immunohistochemical and TUNEL methods, and findings that shed light on clinical applications were obtained day by day.

Keywords

• apoptosis
• Hepatic resection
• Proliferative nuclear antigen
• Regeneration

Project Number

TÜBAB/2011-125

Deneysel Karaciğer Rezeksiyonu Oluşturulmuş Ratlarda Rejenerasyon ve Apoptozun Değerlendirilmesi

Abstract

Giriş ve Amaç: Karaciğer rezeksiyonundan ve transplantasyondan hemen sonra apoptozis ve buna eşlik eden rejenerasyon başlar. DNA sentezinin ise rezeksiyondan sonra ilk 24-48 saatte maksimum düzeye ulaştığı bilinmektedir. Bu çalışma %70 karaciğer rezeksiyonu uygulanan ratların karaciğerinde 3 ve 7. günlerde ki rejenerasyonu ve apoptotik süreci incelemek üzere gerçekleştirildi. Gereç ve Yöntemler: Wistar Albino cinsi erişkin 21 adet erkek sıçan kullanıldı. Karaciğer rezeksiyonu oluşturmak için deneklere üst orta hat insizyon ile laparotomi uygulandı. Kontrol (Grup I), rezeksiyondan 3 gün sonra sakrifiye edilen grup (Grup II) ve rezeksiyondan 7 gün sonra sakrifiye edilen grup (Grup III) olacak şekilde toplam 3 grup oluşturuldu. Bulgular: Kontrol grubu dışında tüm deney gruplarının karaciğer dokularında sinüzoidal dilatasyon ve hepatositlerde vakuolizasyon görüldü. Ancak, bu histopatolojik bulguların 2. grupta diğer gruplardan daha fazla olduğu gözlemlendi. Apoptotik indeks ve proliferasyon indeks değerlerinin 2. grupta maksimum seviyeye ulaştığı görüldü. 3. grupta ise bu indeks değerlerinin giderek azaldığı görülmüştür. Kontrol grubunda bu parametrelere ait değerler oldukça düşük değerlerde bulunmuştur. Sonuç: Sonuç olarak, rezeksiyon sonrası meydana gelen rejenerasyona eşlik eden mekanizmaların günlere göre karşılaştırılmaları immünohistokimyasal ve TUNEL metodlarıyla tespit edilmiş olup klinik uygulamalara ışık tutacak bulgular elde edilmiştir.

Keywords

• Apoptozis
• Hepatik rezeksiyon
• Proliferatif nükleer antijen
• Rejenerasyon

Supporting Institution

Trakya Üniversitesi Bilimsel Araştırmalar Proje Birimi

Project Number

TÜBAB/2011-125

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