Kitosan Bazlı Yüzeylerin Mezenşimal Kök Hücrelerin Nöronal ve Glial Yönde Farklılaşmalarına Etkileri

Yıl 2017, Cilt: 4 Sayı: 1, 549 - 566, 31.03.2017

Yaşar Mehmet Şenses Aylin Şendemir Ürkmez

Öz

Doku mühendisliği, sinir sisteminin rejenerasyonu konusunda ümit vadeden
yeni tekniklerden biridir.  Biyouyumlu ve
biyobozunur doku iskelelerinin kök hücreler için taşıyıcı olarak kullanıldığı
bu yöntemde, seçilen doku iskelesinin kimyasal ve fiziksel özellikleri, üzerine
ekilen hücrelerin davranışlarını yönlendirerek tedavi sürecine katkıda
bulunabilmektedir.

Bu çalışmada biyouyumluluğu çok farklı dokular için gösterilmiş bir
biyopolimer olan kitosan ile sülfat ve fosfat gruplarıyla fonksiyonelleştirilmiş
türevlerinin sinir doku mühendisliği çalışmalarında kullanılma amacıyla sıçan
kemik iliği kökenli mezenşimal kök hücrelerin (MKH) nöronal ve glial yönde
farklılaşmalarına etkileri incelenmiştir.

Hücre kültürü çalışmaları için sıçan kemik iliğinden MKH'ler izole
edilmiş, osteojenik ve kondrojenik hücrelere farklılaşmaları sağlanarak
hücrelerin farklılaşma potansiyelleri kalitatif yöntemlerle gösterilmiştir.
Yüzey kimyasına hassas olduğu bilinen MKH’lerin kitosan, kitosan fosfat ve
kitosan sülfat yüzeyler üzerinde tutunma ve proliferasyon özellikleri
incelenmiştir. Kitosan ve kitosan fosfat yüzeylerde hücre tutunması düşükken,
sülfat fonksiyonel grubunun eklenmesinin hücre tutunmasını anlamlı şekilde
arttırdığı belirlenmiştir. MKH’ler kitosan sülfat yüzeyler üzerinde hem
nöronal hem de glial yönde farklılaştırılabilmişlerdir. Bu çalışma, sinir doku
rejenerasyonu ve doku mühendisliği amacıyla kitosan sülfat yüzey kimyasının
kullanılabileceğini göstermektedir.

Anahtar Kelimeler

Mezenşimal Kök Hücre, Kitosan, Kitosan SüLfat, Sinir Doku Mühendisliği

Kaynakça

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