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Evaluation of Endothelial Nitric Oxide Synthase Gene Polymorphism (T-786 C) in Patients with Slow Coronary Flow

Year 2021, Volume: 8 Issue: 4, 703 - 708, 31.12.2021
https://doi.org/10.34087/cbusbed.1018785

Abstract

Objective: Slow coronary flow (SCF) is characterized by delay of opacification of coronary arteries in coronary angiography in the absence of any evident obstructive lesion. Its pathophysiological mechanisms are uncertain. Several hypotheses have been suggested for SCF, including a form of early phase of atherosclerosis, microvascular dysfunction, inflammation, imbalance between vasoconstrictor and vasodilatory factors, and platelet function disorder. Endothelial nitric oxide synthase (eNOS) gene T-786 C polymorphism have been reported to be associated with many vascular disease. The aim of this study was to investigate the association between SCF and eNOS gene T-786 C polymorphism.
Materials and Methods: Forty patients with SCF and otherwise normal coronary arteries (mean age 52±9 years), 35 patients with coronsry artery disease (CAD) (mean age 55±9 years) and 30 patients with normal coronary angiograms (mean age 51±8 years) were included in the study. TIMI frame count ≥40 frames for the left anterior descending artery was considered as SCF. T-786 C polymorphisms of the eNOS gene were analysed by polymerase chain reaction. Demographic characteristics and major risk factors for atherosclerosis were evaluated in the study groups. The severity of SCF and CAD was assessed based on the number of involved vessel.
Results: There was no significant difference with respect to age and gender between groups. The percentage of smoking was higher in the CAD group than in the SCF and control groups. There was no statistical difference in genotype distribution among the groups. The genotype distribution in SCF group was as follows: TT genotype frequency was 25 (62,5%)k, TC genotype frequency was 12 (30%) and CC genotype frequency was 3 (7,5%). The genotype distribution in CAD group was as follows: TT genotype frequency was 16 (45,7%), TC genotype frequency was 16 (45,7%) and CC genotype frequency was 3 (8,5%). The genotype distribution in control group was as follows: TT genotype frequency was 17 (56,6%), TC genotype frequency was 10 (33,3%) and CC genotype frequency was 3 (10%). In the dominant and recessive models of statistical analysis, there was no statistically significant difference among groups.
Conclusions: Our findings show that there is no significant association between T-786 C polymorphism of eNOS gene and SCF in the present study.

References

  • Tambe, A.A, Demany, M.A, Zimmerman, H.A, Mascarenhas, E, Angina pectoris and slow flow velocity of dye in coronary arteries--a new angiographic finding, American heart journal, 1972, 84(1), 66-71.
  • Li, J.J, Xu, B, Li, Z.C, Qian, J, Wei, B.Q, Is slow coronary flow associated with inflammation? Medical hypotheses, 2006, 66(3), 504-8.
  • Mangieri, E, Macchiarelli, G, Ciavolella, M, Barilla, F, Avella, A, Martinotti, A, et al., Slow coronary flow: clinical and histopathological features in patients with otherwise normal epicardial coronary arteries, Catheterization and cardiovascular diagnosis, 1996, 37(4), 375-81.
  • Yücel, H, Şenarslan, D.A, Koroner Arter Baypas Greft (KABG) Ameliyatı Olan Hastalarda Aterosklerozun İlerlemesi ile Hematolojik Parametreler Arasındaki İlişki, Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi, Cilt 7, Sayı 1, 2020, 29 – 34.
  • Sezgin, N, Barutcu, I, Sezgin, A.T, Gullu, H, Turkmen, M, Esen, A.M, et al., Plasma nitric oxide level and its role in slow coronary flow phenomenon, International heart journal, 2005, 46(3), 373-82.
  • Camsarl, A, Pekdemir, H, Cicek, D, Polat, G, Akkus, M.N, Doven, O, et al., Endothelin-1 and nitric oxide concentrations and their response to exercise in patients with slow coronary flow, Circulation journal, 2003, 67(12), 1022-8.
  • Burckhartt, B.A, Mukerji, V, Alpert, M.A, Coronary artery slow flow associated with angina pectoris and hypotension--a case report, Angiology, 1998, 49(6), 483-7.
  • Sarak, T, Karadeniz, M, Akut Koroner Sendromlu Hastalarda Kronik Total Oklüzyon Sıklığı ve Risk Faktörleriyle İlişkisi, Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi, Cilt 6, Sayı 3, 2019, 194 – 198.
  • Liaudet, L, Soriano, F.G, Szabo, C, Biology of nitric oxide signaling, Critical care medicine, 2000, 28(4 Suppl), N37-52.
  • Mayer, B, Hemmens, B, Biosynthesis and action of nitric oxide in mammalian cells, Trends in biochemical sciences, 1997, 22(12), 477-81.
  • Marsden, P.A, Heng, H.H, Scherer, S.W, Stewart, R.J, Hall, A.V, Shi, X.M, et al., Structure and chromosomal localization of the human constitutive endothelial nitric oxide synthase gene, The Journal of biological chemistry, 1993, 268(23), 17478-88.
  • Wattanapitayakul, S.K, Mihm, M.J, Young, A.P, Bauer, J.A, Therapeutic implications of human endothelial nitric oxide synthase gene polymorphism, Trends in pharmacological sciences, 2001, 22(7), 361-8.
  • Rossi, G.P, Cesari, M, Zanchetta, M, Colonna, S, Maiolino, G, Pedon, L, et al., The T-786C endothelial nitric oxide synthase genotype is a novel risk factor for coronary artery disease in Caucasian patients of the GENICA study, Journal of the American College of Cardiology, 2003, 41(6), 930-7.
  • Kuhlencordt, P.J, Gyurko, R, Han, F, Scherrer-Crosbie, M, Aretz, T.H, Hajjar, R, et al., Accelerated atherosclerosis, aortic aneurysm formation, and ischemic heart disease in apolipoprotein E/endothelial nitric oxide synthase double-knockout mice, Circulation, 2001, 104(4), 448-54.
  • Caglayan, A.O, Kalay, N, Saatci, C, Yalcyn, A, Akalyn, H, Dundar, M, Lack of association between the Glu298Asp polymorphism of endothelial nitric oxide synthase and slow coronary flow in the Turkish population, The Canadian journal of cardiology, 2009, 25(3), e69-72.
  • Nurkalem, Z, Tangurek, B, Zencirci, E, Alper, A.T, Aksu, H, Erer, B, et al., Endothelial nitric oxide synthase gene (T-786C) polymorphism in patients with slow coronary flow, Coronary artery disease, 2008, 19(2), 85-8.
  • Gupta, M.D, Akkarappatty, C, Girish, M.P, Kumar, R, Rain, M, Tyagi, S, Qadar Pasha, M.A, Association between the Glu298Asp and 4b/4a polymorphisms of endothelial nitric oxide synthase and coronary slow flow in the North Indian population, Coronary artery disease, 2014, 25(3), 192-7.
  • Rossi, G.P, Maiolino, G, Zanchetta, M, Sticchi, D, Pedon, L, Cesari, M, et al., The T (-786)C endothelial nitric oxide synthase genotype predicts cardiovascular mortality in high-risk patients, Journal of the American College of Cardiology, 2006, 48(6), 1166-74.
  • Tajouri, L, Martin, V, Ovcaric, M, Curtain, R.P, Lea, R.A, Csurhes, P, et al., Investigation of an inducible nitric oxide synthase gene (NOS2A) polymorphism in a multiple sclerosis population, Brain research bulletin, 2004, 64(1), 9-13.
  • Hyndman, M.E, Parsons, H.G, Verma, S, Bridge, P.J, Edworthy, S, Jones, C, et al., The T-786-->C mutation in endothelial nitric oxide synthase is associated with hypertension, Hypertension, 2002, 39(4), 919-22.
  • Monti, L.D, Barlassina, C, Citterio, L, Galluccio, E, Berzuini, C, Setola, E, et al., Endothelial nitric oxide synthase polymorphisms are associated with type 2 diabetes and the insulin resistance syndrome, Diabetes, 2003, 52(5), 1270-5.

Yavaş Koroner Akımı Olan Hastalarda Endotelyal Nitrik Oksit Sentaz Gen Polimorfizmin (T-786C) Araştırılması

Year 2021, Volume: 8 Issue: 4, 703 - 708, 31.12.2021
https://doi.org/10.34087/cbusbed.1018785

Abstract

Giriş ve Amaç: Yavaş koroner akım (YKA), koroner anjiyografide tıkayıcı lezyon yokluğunda koroner arterlerde opak maddenin gecikmesiyle karakterizedir. Patofizyolojik mekanizmaları belirsizdir. YKA için çeşitli hipotezler sürülmüştür; aterosklerozun erken fazının bir formu, mikrovasküler disfoksiyon, inflamasyon, vazokonstriktör ve vazodilatör faktörler arasındaki dengesizlik ve tromobist fonksiyon bozukluğudur. Endotelyal nitrik oksit sentaz (eNOS) geni T-786C polimorfizminin birçok damarsal hastalıkla ilişkisi bildirilmiştir. Bu çalışmanın amacı YKA ile eNOS gen T-786C polimorfizmi arasındaki ilişkiyi araştırmaktır.
Gereç ve Yöntemler: Koroner arterleri normal ancak YKA olan 40 hasta (ortalama yaş 52 ± 9 yıl), koroner arter hastalığı (KAH) olan 35 hasta (ortalama yaş 55 ± 9 yıl) ve koroner anjiyografisi normal olan 30 hasta (ortalama yaş 51 ± 8 yıl) çalışmaya alındı. Sol ön inen arterde, TIMI kare sayısı 40 ve üzeri olması YKA olarak kabul edildi. eNOS geni T-786C polimorfizmleri polimeraz zincir reaksiyonu ile analiz edildi. Çalışma gruplarının demografik özellikleri ve ateroskleroz için major risk faktörleri değerlendirildi. YKA ve KAH ciddiyeti etkilenen damar sayısına göre değerlendirildi.
Bulgular: Gruplar arasında yaş ve cinsiyet açısından anlamlı fark yoktu. Sigara içme yüzdesi, KAH grubunda diğer gruplardan daha yüksekti. Gruplar arasında genotip dağılımında istatiksel olarak anlamlı fark yoktu. YKA grubunda genotip dağılımı; TT genotip sıklığı 25 (%62,5), TC genotip sıklığı 12 (%30) ve CC genotip sıklığı 3’tü (%7,5). KAH grubunda genotip dağılımı; TT genotip sıklığı 16 (%45,7), TC genotip sıklığı 16 (%45,7) ve CC genotip sıklığı 3’tü (%8,5). Kontrol grubunda genetip dağılımı; TT genotip sıklığı 17 (%56,6), TC genotip sıklığı 10 (%33,3) ve CC genotip sıklığı 3’tü (%10). Dominat ve resesif modellerin istatiksel analizinde gruplar arasında anlamlı bir fark yoktu.
Sonuç: Bulgularımız eNOS gen T-786C polimorfizmi ile YKA arasında anlamı bir ilişki olmadığını göstermektedir.

References

  • Tambe, A.A, Demany, M.A, Zimmerman, H.A, Mascarenhas, E, Angina pectoris and slow flow velocity of dye in coronary arteries--a new angiographic finding, American heart journal, 1972, 84(1), 66-71.
  • Li, J.J, Xu, B, Li, Z.C, Qian, J, Wei, B.Q, Is slow coronary flow associated with inflammation? Medical hypotheses, 2006, 66(3), 504-8.
  • Mangieri, E, Macchiarelli, G, Ciavolella, M, Barilla, F, Avella, A, Martinotti, A, et al., Slow coronary flow: clinical and histopathological features in patients with otherwise normal epicardial coronary arteries, Catheterization and cardiovascular diagnosis, 1996, 37(4), 375-81.
  • Yücel, H, Şenarslan, D.A, Koroner Arter Baypas Greft (KABG) Ameliyatı Olan Hastalarda Aterosklerozun İlerlemesi ile Hematolojik Parametreler Arasındaki İlişki, Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi, Cilt 7, Sayı 1, 2020, 29 – 34.
  • Sezgin, N, Barutcu, I, Sezgin, A.T, Gullu, H, Turkmen, M, Esen, A.M, et al., Plasma nitric oxide level and its role in slow coronary flow phenomenon, International heart journal, 2005, 46(3), 373-82.
  • Camsarl, A, Pekdemir, H, Cicek, D, Polat, G, Akkus, M.N, Doven, O, et al., Endothelin-1 and nitric oxide concentrations and their response to exercise in patients with slow coronary flow, Circulation journal, 2003, 67(12), 1022-8.
  • Burckhartt, B.A, Mukerji, V, Alpert, M.A, Coronary artery slow flow associated with angina pectoris and hypotension--a case report, Angiology, 1998, 49(6), 483-7.
  • Sarak, T, Karadeniz, M, Akut Koroner Sendromlu Hastalarda Kronik Total Oklüzyon Sıklığı ve Risk Faktörleriyle İlişkisi, Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi, Cilt 6, Sayı 3, 2019, 194 – 198.
  • Liaudet, L, Soriano, F.G, Szabo, C, Biology of nitric oxide signaling, Critical care medicine, 2000, 28(4 Suppl), N37-52.
  • Mayer, B, Hemmens, B, Biosynthesis and action of nitric oxide in mammalian cells, Trends in biochemical sciences, 1997, 22(12), 477-81.
  • Marsden, P.A, Heng, H.H, Scherer, S.W, Stewart, R.J, Hall, A.V, Shi, X.M, et al., Structure and chromosomal localization of the human constitutive endothelial nitric oxide synthase gene, The Journal of biological chemistry, 1993, 268(23), 17478-88.
  • Wattanapitayakul, S.K, Mihm, M.J, Young, A.P, Bauer, J.A, Therapeutic implications of human endothelial nitric oxide synthase gene polymorphism, Trends in pharmacological sciences, 2001, 22(7), 361-8.
  • Rossi, G.P, Cesari, M, Zanchetta, M, Colonna, S, Maiolino, G, Pedon, L, et al., The T-786C endothelial nitric oxide synthase genotype is a novel risk factor for coronary artery disease in Caucasian patients of the GENICA study, Journal of the American College of Cardiology, 2003, 41(6), 930-7.
  • Kuhlencordt, P.J, Gyurko, R, Han, F, Scherrer-Crosbie, M, Aretz, T.H, Hajjar, R, et al., Accelerated atherosclerosis, aortic aneurysm formation, and ischemic heart disease in apolipoprotein E/endothelial nitric oxide synthase double-knockout mice, Circulation, 2001, 104(4), 448-54.
  • Caglayan, A.O, Kalay, N, Saatci, C, Yalcyn, A, Akalyn, H, Dundar, M, Lack of association between the Glu298Asp polymorphism of endothelial nitric oxide synthase and slow coronary flow in the Turkish population, The Canadian journal of cardiology, 2009, 25(3), e69-72.
  • Nurkalem, Z, Tangurek, B, Zencirci, E, Alper, A.T, Aksu, H, Erer, B, et al., Endothelial nitric oxide synthase gene (T-786C) polymorphism in patients with slow coronary flow, Coronary artery disease, 2008, 19(2), 85-8.
  • Gupta, M.D, Akkarappatty, C, Girish, M.P, Kumar, R, Rain, M, Tyagi, S, Qadar Pasha, M.A, Association between the Glu298Asp and 4b/4a polymorphisms of endothelial nitric oxide synthase and coronary slow flow in the North Indian population, Coronary artery disease, 2014, 25(3), 192-7.
  • Rossi, G.P, Maiolino, G, Zanchetta, M, Sticchi, D, Pedon, L, Cesari, M, et al., The T (-786)C endothelial nitric oxide synthase genotype predicts cardiovascular mortality in high-risk patients, Journal of the American College of Cardiology, 2006, 48(6), 1166-74.
  • Tajouri, L, Martin, V, Ovcaric, M, Curtain, R.P, Lea, R.A, Csurhes, P, et al., Investigation of an inducible nitric oxide synthase gene (NOS2A) polymorphism in a multiple sclerosis population, Brain research bulletin, 2004, 64(1), 9-13.
  • Hyndman, M.E, Parsons, H.G, Verma, S, Bridge, P.J, Edworthy, S, Jones, C, et al., The T-786-->C mutation in endothelial nitric oxide synthase is associated with hypertension, Hypertension, 2002, 39(4), 919-22.
  • Monti, L.D, Barlassina, C, Citterio, L, Galluccio, E, Berzuini, C, Setola, E, et al., Endothelial nitric oxide synthase polymorphisms are associated with type 2 diabetes and the insulin resistance syndrome, Diabetes, 2003, 52(5), 1270-5.
There are 21 citations in total.

Details

Primary Language Turkish
Subjects Cardiovascular Surgery
Journal Section Araştırma Makalesi
Authors

Habil Yücel 0000-0002-7141-4775

Abdullah Doğan 0000-0001-8163-2365

Publication Date December 31, 2021
Published in Issue Year 2021 Volume: 8 Issue: 4

Cite

APA Yücel, H., & Doğan, A. (2021). Yavaş Koroner Akımı Olan Hastalarda Endotelyal Nitrik Oksit Sentaz Gen Polimorfizmin (T-786C) Araştırılması. Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi, 8(4), 703-708. https://doi.org/10.34087/cbusbed.1018785
AMA Yücel H, Doğan A. Yavaş Koroner Akımı Olan Hastalarda Endotelyal Nitrik Oksit Sentaz Gen Polimorfizmin (T-786C) Araştırılması. CBU-SBED: Celal Bayar University-Health Sciences Institute Journal. December 2021;8(4):703-708. doi:10.34087/cbusbed.1018785
Chicago Yücel, Habil, and Abdullah Doğan. “Yavaş Koroner Akımı Olan Hastalarda Endotelyal Nitrik Oksit Sentaz Gen Polimorfizmin (T-786C) Araştırılması”. Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi 8, no. 4 (December 2021): 703-8. https://doi.org/10.34087/cbusbed.1018785.
EndNote Yücel H, Doğan A (December 1, 2021) Yavaş Koroner Akımı Olan Hastalarda Endotelyal Nitrik Oksit Sentaz Gen Polimorfizmin (T-786C) Araştırılması. Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi 8 4 703–708.
IEEE H. Yücel and A. Doğan, “Yavaş Koroner Akımı Olan Hastalarda Endotelyal Nitrik Oksit Sentaz Gen Polimorfizmin (T-786C) Araştırılması”, CBU-SBED: Celal Bayar University-Health Sciences Institute Journal, vol. 8, no. 4, pp. 703–708, 2021, doi: 10.34087/cbusbed.1018785.
ISNAD Yücel, Habil - Doğan, Abdullah. “Yavaş Koroner Akımı Olan Hastalarda Endotelyal Nitrik Oksit Sentaz Gen Polimorfizmin (T-786C) Araştırılması”. Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi 8/4 (December 2021), 703-708. https://doi.org/10.34087/cbusbed.1018785.
JAMA Yücel H, Doğan A. Yavaş Koroner Akımı Olan Hastalarda Endotelyal Nitrik Oksit Sentaz Gen Polimorfizmin (T-786C) Araştırılması. CBU-SBED: Celal Bayar University-Health Sciences Institute Journal. 2021;8:703–708.
MLA Yücel, Habil and Abdullah Doğan. “Yavaş Koroner Akımı Olan Hastalarda Endotelyal Nitrik Oksit Sentaz Gen Polimorfizmin (T-786C) Araştırılması”. Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi, vol. 8, no. 4, 2021, pp. 703-8, doi:10.34087/cbusbed.1018785.
Vancouver Yücel H, Doğan A. Yavaş Koroner Akımı Olan Hastalarda Endotelyal Nitrik Oksit Sentaz Gen Polimorfizmin (T-786C) Araştırılması. CBU-SBED: Celal Bayar University-Health Sciences Institute Journal. 2021;8(4):703-8.