N-Acetylcysteine Ameliorates 5-Fluorouracil‐Induced Ovarian Injury in Rats

Year 2023, , 776 - 781, 29.12.2023

Elif Ayazoglu Demir , Ahmet Mentese , Hatice Küçük , Nihal Türkmen , Selim Demir , Yüksel Aliyazıcıoğlu

https://doi.org/10.33808/clinexphealthsci.1199615

Cited By: 2

Abstract

Objective: Although 5-fluorouracil (5-FU) is one of the most commonly used chemotherapeutics worldwide, it has been shown that 5-FU administration can cause reproductive toxicity in recent years. N-acetylcysteine (NAC) is the precursor of glutathione, the most important endogenous antioxidant molecule and is known for its effective antioxidant and anti-inflammatory properties. Although NAC is one of the most studied antioxidant molecules, its curative effect against ovarian damage caused by 5-FU has not been demonstrated to date. It was therefore aimed to investigate whether NAC is therapeutic against 5-FU-induced ovotoxicity in this study for the first time.
Methods: Rats were first exposed to a single dose of 5-FU (100 mg/kg) and then treated with NAC (10 and 20 mg/kg) for three days. The oxidative stress, inflammation and apoptosis markers in ovarian tissues were also determined using spectrophotometric methods. Ovarian tissues were also evaluated histologically.
Results: It was revealed that the levels of oxidative stress, inflammation and apoptosis biomarkers in ovarian tissue increased by 5-FU administration (p<.005). Treatments with NAC significantly restored these damages dose-dependently (p<.005). Moreover, these biochemical findings were confirmed by histological examination.
Conclusion: NAC can be considered as a potential therapeutic molecule against 5-FU-induced reproductive toxicity, as it can abolish the ovarian toxicity caused by 5-FU by reducing oxidative stress, inflammation and apoptosis.

Keywords

5-Fluorouracil, Apoptosis, Inflammation, N-acetylcysteine, Oxidative stress, Ovotoxicity

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