Effect of Hypochlorous Acid on Acrylic Resins in Preventing Cross-infection Between Clinic and Laboratory
Year 2025,
Volume: 15 Issue: 1, 226 - 231, 28.03.2025
Fulya Basmacı
,
Özge Uslu Akçam
,
Tuğgen Özcivelek
Abstract
Objective: The COVID-19 pandemic and monkeypox disease have highlighted the importance of preventing cross-infection between dental laboratories and clinics. Thus, the aim of this study was to evaluate the effects of hypochlorous acid (HOCL), a natural disinfectant that is effective on bacteria and viruses, on polymethylmetacrylate (PMMA) to prevent cross-infection.
Methods: A total of 120 PMMA (60 heat-activated and 60 three dimensionally printed) samples were prepared. The samples were immersed in HOCL and ortophtalaldehyde (OPA) disinfectant twice for 30 minutes to simulate the travel time to clinic and the dental laboratory. 10 samples from each group were kept in deionized water as a control group. Flexural strength and surface roughness measurements of the samples were performed. T-test and Kruskal Wallis H test were used for statistical evaluation (α=0.05).
Results: There was no significant difference in flexural strength or Ra values between the HOCL and OPA groups.
Conclusion: HOCL PMMA may be considered an alternative disinfectant for preventing cross-infection between dental laboratories and clinics in dentures and orthodontic appliances. Further research is required to evaluate its biological efficacy and long-term use.
Ethical Statement
This invitro study dosen't need approval from the Ethics committee.
References
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https://doi.org/10.1016/s0022-3913(05)80140-4
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https://doi.org/10.1016/j.joms.2020.06.029
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https://doi.org/10.1111/j.1532-849X.2006.00131
- Ahila SC, Thulasingam C. Effect of disinfection on gypsum casts retrieved from addition and condensation silicone impressions disinfected by immersion and spray methods.SRM
J Res Dent Sci. 2014; 5(3):163-169.
https://doi.org/10.4103/0976-433X.138724
- Bensel T, Bock JJ, Kebernik A, Arnold C, Mansour S, Boeckler AF. Effect of disinfectants on mechanical properties of orthodontic acrylics. Int.J.of Biomater. 2019; 24:1-10. 1096208.
https://doi.org/10.1155/2019/1096208
- Sartori EA, Schmidt CB, Walber LF, Shinkai RS .Effect of microwave disinfection on denture base adaptation and resin surface roughness. Braz Dent J. 2006; 17(3):195-200. https://doi.org/10.1590/S0103-64402006000300004
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https://doi.org/10.5336/dentalsci.2022-92010
- Koujan A, Aggarwal H, Chen PH, Li Z, Givan DA, Zhang P, Fu CC.Evaluation of candida albicans adherence to CAD-CAM milled, 3D-printed, and heat-cured PMMA resin and efficacy of different disinfection techniques: an in vitro study. J Prosthodont.2023; 32(6): 512-518.
https://doi.org/10.1111/jopr.13583
- Prpić V, Schauperl Z, Ćatić A, Dulčić N, Čimić S. Comparison of mechanical properties of 3D-printed, CAD/CAM, and conventional denture base materials. J Prosthodont. 2020 ;29(6):524-528.
https://doi.org/10.1111/jopr.13175
- Gad, MM, Fouda SM. Factors affecting flexural strength of 3D-printed resins: a systematic review.J Prosthodont.2023; 11:1-15.32(S1):96-110. https://doi.org/10.1111/jopr.13640
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https://doi.org/10.1016/j.dental.2010.01.006
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https://doi.org/10.3390/dj10030042
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https://doi.org/10.1111/jopr.12539
- Gad MM, Abualsaud R, Fouda SM, Rahoma A, Al-Thobity AM, Khan SQ, Akhtar S, Al-Harbi FA. . Effects of denture cleansers on the flexural strength of PMMA denture base resin modified with ZrO2 nanoparticles. J. Prosthodont 2021; 30(3): 235–244. https://doi.org/10.1111/jopr.13234
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- Gad MM, Fouda SM, Abualsaud R, Alshahrani FA, Al-Thobity AM, Khan SQ, Akhtar S, Ateeq IS, Helal MA, Al-Harbi FA. Strength and surface properties of a 3D-printed denture base polymer. J. Prosthodont.2022; 31(5),412–418. https://doi.org/10.1111/jopr.13413
- Alifui-Segbaya F, Bowman J, White AR, George R , Fidan I. Characterization of the double bond conversion of acrylic resins for 3D printing of dental prostheses. Compend Contin Educ Dent.2019; 40(10):7-11.PMID: 31730361
- Al-Dwairi ZN, Al Haj Ebrahim AA, Baba NZ. A Comparison of the surface and mechanical properties of 3D printable denture-base resin material and conventional polymethylmethacrylate (PMMA). J.Prosthodont.2023; 32(1): 40–48. https://doi.org/10.1111/jopr.13491
- Gessi A, Formaglio P, Semeraro B, Summa D, Tamisari E, Tamburini E. Electrolyzed hypochlorous acid (HOCl) aqueous solution as low-impact and eco-friendly agent for floor cleaning and sanitation. Int. J. Environ. Res. Public Health. 2023; 20(18):6712. https://doi.org/10.3390/ijerph20186712
Year 2025,
Volume: 15 Issue: 1, 226 - 231, 28.03.2025
Fulya Basmacı
,
Özge Uslu Akçam
,
Tuğgen Özcivelek
References
- Salvia AC, Matilde Fdos S, Rosa FC, Kimpara ET, Jorge AO, Balducci I, Dis Koga-Ito CY. Disinfection protocols to prevent cross-contamination between dental offices and prosthetic laboratories. J Infect Public Health. 2013; 6(5):377-382.
https://doi.org/10.1016/j.jiph.2013.04.011
- Raszewski Z, Toporowska AN, Nowakowska D, Więckiewicz W.Update on acrylic resins used in dentistry. Mini Rev Med Chem. 2021; 21(15):2130-2137. https://doi.org/10.2174/1389557521666210226151214
- Zafar MS. Prosthodontic applications of polymethyl methacrylate (PMMA): an update. Polymers (Basel) 2020; 12(10):2299.
https://doi.org/10.3390/polym12102299
- Katreva IP, Dikova T, Abadzhiev M, Tonchev T, Dzhendov D, Simov M, Angelova S, Pavlova D, Doychinova M. 3D-printing in contemporary prosthodontic treatment. Scripta Infect Public Health. 2016; 2(1):7-11
- Barazanchi A, Li KC, Al‐Amleh B, Lyons K, Waddell JN. Additive technology: update on current materials and applications in dentistry. J Prosthodont.2017; 26(2):156-163. https://doi.org/10.1111/jopr.12510
- Carvalho CF, Vanderlei AD, Marocho SM, Pereira SM, Nogueira L, Paes-Júnior TJ. Effect of disinfectant solutions on a denture base acrylic resin. Acta Odontol Latinoam. 2012; 25(3): 255-260. PMID: 23798071
- Chau VB, Saunders TR, Pimsler M, Elfring DR. In-depth disinfection of acrylic resins. J Prosthet Dent. 1995 Sep;74(3):309-313.
https://doi.org/10.1016/s0022-3913(05)80140-4
- Yokliant W, Leevarakarn S. Effect of ortho-phthaladehyde disinfectant on color stability of two different acrylic resin teeth using spectrophotometer. J.Dep. Med. Serv.2022; 47(3). 96-103
- Kadhim GF, Jabbar MK, Hameed NY. Hypochlorous acid: effects on two surface properties of denture materials. J.Oral Dent Res. 2022; 9(1):44-52
- Akamatsu T, Minemoto M, Uyeda M. Evaluation of the antimicrobial activity and materials compatibility of orthophthalaldehyde as a high-level disinfectant. J Int Med Res. 2005 ;33(2): 178-187.
https://doi.org/10.1177/147323000503300205
- Block MS, Rowan BG. Hypochlorous acid: a review. J Oral Maxillofac Surg.2020; 78(9): 1461–1466.
https://doi.org/10.1016/j.joms.2020.06.029
- Nguyen K, Bui D, Hashemi M, Hocking DM, Mendis P, Strugnell RA, Dharmage SC. The potential use of hypochlorous acid and a smart prefabricated sanitising chamber to reduce occupation-related COVID-19 exposure. Risk Manag Healthc Policy.2021; 22(14):247-252. https://doi.org/10.2147/RMHP.S284897
- United State Environmental Protection Agency. About list N: disinfectants for coronavirus (COVID-19). 2022. https://www.epa.gov/pesticide-registration. Accessed 23 Dec 2022
- World Health Organization. Cleaning and disinfection of environmental surfaces in the context of COVID-19. 2020. https://www.who.int/publications/i/item/cleaning-and-disinfection-of-environmental-surfaces-inthe-context-of-covid-19. Accessed 23 Dec 2022
- Jasim JM, Abass SM.The effect of hypochlorous acid disinfectant on the reproduction of details and surface hardness of type III dental stone. Cureus.2022; 14(11) 1-7. https://doi.org/10.7759/cureus.32061
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- Srinivasan M, Kalberer N, Kamnoedboon P, Mekki M, Durual S, Ozcan M , Müller F. CAD-CAM complete denture resins: an evaluation of biocompatibility, mechanical properties, and surface characteristics. J Dent. 2021; 114:103785. https://doi.org/10.1016/j.jdent.2021.103785
- Savabi O, Attar K, Nejatidanesh F, Goroohi H, Badrian H .Effect of different chemical disinfectants on the flexural strength of heat-polymerized acrylic resins. Eur J Prosthodont Restor Dent. 2013; 21(3):105-108. PMID: 24261101
- Alkaltham NS, Aldhafiri RA, Al-Thobity AM, Alramadan H, Aljubran H, Ateeq IS, Khan SQ, Akhtar S, Gad MM. Effect of denture disinfectants on the mechanical performance of 3D-printed denture base materials. Polymers. 2023; 15(5):1175. https://doi.org/10.3390/polym15051175
- Ellakwa AE, El-Sheikh AM. Effect of chemical disinfectants and repair materials on the transverse strength of repaired heat-polymerized acrylic resin. J Prosthodont. 2006; 15(5):300-305.
https://doi.org/10.1111/j.1532-849X.2006.00131
- Ahila SC, Thulasingam C. Effect of disinfection on gypsum casts retrieved from addition and condensation silicone impressions disinfected by immersion and spray methods.SRM
J Res Dent Sci. 2014; 5(3):163-169.
https://doi.org/10.4103/0976-433X.138724
- Bensel T, Bock JJ, Kebernik A, Arnold C, Mansour S, Boeckler AF. Effect of disinfectants on mechanical properties of orthodontic acrylics. Int.J.of Biomater. 2019; 24:1-10. 1096208.
https://doi.org/10.1155/2019/1096208
- Sartori EA, Schmidt CB, Walber LF, Shinkai RS .Effect of microwave disinfection on denture base adaptation and resin surface roughness. Braz Dent J. 2006; 17(3):195-200. https://doi.org/10.1590/S0103-64402006000300004
- Bulut AC, Yurksayar AAD. Effect of eight different disinfection methods on the surface roughness and flexural strength properties of acrylic resins: In vitro study. Turkiye Klinikleri J Dental Sci.2023;29 (1): 139-146.
https://doi.org/10.5336/dentalsci.2022-92010
- Koujan A, Aggarwal H, Chen PH, Li Z, Givan DA, Zhang P, Fu CC.Evaluation of candida albicans adherence to CAD-CAM milled, 3D-printed, and heat-cured PMMA resin and efficacy of different disinfection techniques: an in vitro study. J Prosthodont.2023; 32(6): 512-518.
https://doi.org/10.1111/jopr.13583
- Prpić V, Schauperl Z, Ćatić A, Dulčić N, Čimić S. Comparison of mechanical properties of 3D-printed, CAD/CAM, and conventional denture base materials. J Prosthodont. 2020 ;29(6):524-528.
https://doi.org/10.1111/jopr.13175
- Gad, MM, Fouda SM. Factors affecting flexural strength of 3D-printed resins: a systematic review.J Prosthodont.2023; 11:1-15.32(S1):96-110. https://doi.org/10.1111/jopr.13640
- Bettencourt AF, Neves CB, de Almeida MS, Pinheiro LM, Oliveira SA,Lopes, LP, Castro MF. Biodegradation of acrylic based resins: a review. Dent. Mater.2010; 26(5): 171-180.
https://doi.org/10.1016/j.dental.2010.01.006
- Gad MM, Alshehri SZ, Alhamid SA, Albarrak A, Khan SQ, Alshahrani FA, Alqarawi FK. Water sorption, solubility, and translucency of 3D-printed denture base resins. Dent. J.2022; 10(3):1-13.
https://doi.org/10.3390/dj10030042
- Kurt A, Erkose-Genc G, Uzun M, Sarı T, Isik-Ozkol G. The effect of cleaning solutions on a denture base material: elimination of candida albicans and alteration of physical properties. J. Prosthodont.2018;27(6): 577–583.
https://doi.org/10.1111/jopr.12539
- Gad MM, Abualsaud R, Fouda SM, Rahoma A, Al-Thobity AM, Khan SQ, Akhtar S, Al-Harbi FA. . Effects of denture cleansers on the flexural strength of PMMA denture base resin modified with ZrO2 nanoparticles. J. Prosthodont 2021; 30(3): 235–244. https://doi.org/10.1111/jopr.13234
- Freitas RFCP, Duarte S, Feitosa S, Dutra V, Lin WS, Panariello BHD, Carreiro ADFP. Physical, mechanical, and anti-biofilm formation properties of CAD-CAM milled or 3D printed denture base resins: in vitro analysis. J. Prosthodont.2023; 32(S1):38-44. https://doi.org/10.1111/jopr.13554
- Gad MM, Fouda SM, Abualsaud R, Alshahrani FA, Al-Thobity AM, Khan SQ, Akhtar S, Ateeq IS, Helal MA, Al-Harbi FA. Strength and surface properties of a 3D-printed denture base polymer. J. Prosthodont.2022; 31(5),412–418. https://doi.org/10.1111/jopr.13413
- Alifui-Segbaya F, Bowman J, White AR, George R , Fidan I. Characterization of the double bond conversion of acrylic resins for 3D printing of dental prostheses. Compend Contin Educ Dent.2019; 40(10):7-11.PMID: 31730361
- Al-Dwairi ZN, Al Haj Ebrahim AA, Baba NZ. A Comparison of the surface and mechanical properties of 3D printable denture-base resin material and conventional polymethylmethacrylate (PMMA). J.Prosthodont.2023; 32(1): 40–48. https://doi.org/10.1111/jopr.13491
- Gessi A, Formaglio P, Semeraro B, Summa D, Tamisari E, Tamburini E. Electrolyzed hypochlorous acid (HOCl) aqueous solution as low-impact and eco-friendly agent for floor cleaning and sanitation. Int. J. Environ. Res. Public Health. 2023; 20(18):6712. https://doi.org/10.3390/ijerph20186712