Abstract

Van Gölü balığı, Van Gölü'nde yaşayan az sayıdaki omurgalı türlerinden biridir bu yüzden gölün kirliliğinin belirlenmesinde çok önemli bir gösterge olarak rol almaktadır. Deltamethrin (DLT), Van Gölü havzasında yaygın olarak kullanılan pestisitlerden biridir. Bu çalışmada, DLT'nin primer solungaç hücre kültürü üzerine etkilerinin belirlenmesi amaçlanmıştır. Primer solungaç hücre kültürüne farklı konsantrasyonlarda (0.01, 0.1, 1 ve 10 µM) eklenen DLT’nin 24 ve 48 saat sonunda toplam antioksidan, oksidan, malondialdehit (MDA) ve 8-hidroksi-2'-deoksiguanozin (8-OHdG) seviyeleri belirlenmiştir. Uygulanan en yüksek DLT konsantrasyonu (10 µM) 48 saatte toplam oksidan seviyesinde önemli bir artışa neden olurken, 24 ve 48 saatte toplam antioksidan seviyesinde bir azalma tespit edildi (P < 0.05). Doymamış yağ asitlerinin lipid peroksidasyonunun ürünü olan MDA seviyesindeki artış, 24 ve 48 saat sonunda 1-10 µM DLT konsantrasyonları ile anlamlı bulunmuştur (P < 0.05). DLT uygulama konsantrasyonlarının hiçbirinde 8-OHdG seviyesinde herhangi bir değişiklik gözlenmedi (P > 0.05). Sonuç olarak, 1 ve 10 µM konsantrasyonlara maruz bırakılan Van Gölü balıklarının primer solungaç hücre kültüründe DLT'nin oksidatif strese neden olduğu ancak herhangi bir genotoksik etkinin gözlenmediği söylenebilir.

Determination of toxic effects of deltamethrin on the primary gill cell culture of Lake Van fish [Alburnus tarichi (Güldenstadt 1814)]

Abstract

Lake Van fish is one of few vertebrate species living in the lake, therefore it plays a very useful role as an indicator in determining lake pollution. Deltamethrin (DLT) is one of the pesticides used extensively in the Lake Van basin. In this study, it was aimed to determine the effects of DLT on the primary gill cell culture. Different concentrations of DLT (0.01, 0.1, 1, and 10 µM) were added to primary gill cell culture and the total antioxidant and oxidant levels, malondialdehyde (MDA), and 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels were determined at the end of 24 and 48 h of administration. While the highest concentration of applied DLT (10 µM) caused a significant increase in the total oxidant level at 48 h, a decrease in the total antioxidant level was detected at 24 and 48 h (P < 0.05). The increase in MDA level, which is the product of lipid peroxidation of unsaturated fatty acids, with DLT concentrations of 1–10 µM at the end of 24 and 48 h was found to be significant (P < 0.05). As a result, it can be said that DLT caused oxidative stress in the primary gill cell culture of Lake Van fish exposed to concentrations of 1 and 10 µM, but no genotoxic effect was observed.

Keywords

• Pesticides
• Oxidative stress
• Lipid peroxidation
• DNA damage
• Lake Van

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