Assessment of The Effect of Thymbra capitata Ethanolic Extract on Galleria mellonella Hemolymph Antioxidant Enzymes

Year 2022, , 178 - 183, 31.12.2022

Serhat Kaya

https://doi.org/10.31594/commagene.1204178

Cited By: 3

Abstract

Conehead thyme (Thymbra capitata) is widely distributed in the countries of the Mediterranean region and used due to its medical properties. The antibacterial, antifungal, and strong antioxidant properties of T. capitata are known. The model organism Galleria mellonella is mostly preferred for immunological studies and for the study of human pathogens. The aim of the study was to determine the effect of the ethanolic extract of T. capitata on the antioxidant defense of the hemolymph in G. mellonella larva. Solutions prepared with Phosphate-Buffered Saline (PBS) from the dry matter obtained from ethanolic extract at doses between 2 mg mL-1 and 20 mg mL-1 were injected into G. mellonella larvae. According to our findings, T. capitata extract had no effect on malondialdehyde (MDA) levels. However, it was determined that all doses between 10 to 20 mg mL-1 significantly reduced superoxide dismutase (SOD) and catalase (CAT) activities compared to the control groups. According to the results of our study, high doses of T. capitata extract had negative effects on G. mellonella antioxidant defense.

Keywords

Malondialdehyde, superoxide dismutase, catalase, total protein.

Supporting Institution

Yok

Project Number

Yok

Thanks

The author would like to thank Begüm Çetin for her support and COMUDAM (Çanakkale Onsekiz Mart University Experimental Research Application and Research Center) staff and managers for their precious help.

Thymbra capitata Etanolik Ekstraktının Galleria mellonella Hemolenf Antioksidan Enzimleri Üzerine Etkisinin Değerlendirilmesi

Abstract

Acı kekik (Thymbra capitata), Akdeniz bölgesi ülkelerinde geniş yayılış göstermektedir ve medikal özelliklerinden dolayı kullanımı yaygındır. T. capitata'nın antibakteriyel, antifungal ve güçlü antioksidan özellikleri bilinmektedir. Model organizma Galleria mellonella bağışıklık araştırmaları için ve insan patojenlerinin araştırılmasında çoğunlukla tercih edilmektedir. Çalışmanın amacı, T. capitata'nın etanolik ekstraktının G. mellonella larvasında hemolenf antioksidan savunması üzerindeki etkisinin belirlenmesidir. Etanolik ekstraktan elde edilen kuru maddeden Fosfat Tampon Tuzu (PBS) ile 2 mg mL-1 ile 20 mg mL-1 arasındaki dozlarda hazırlanan çözeltiler G. mellonella larvalarına enjekte edilmiştir. Bulgularımıza göre T. capitata ekstraktının malondialdehit (MDA) düzeylerine etkisi yoktur. Ancak 10 - 20 mg mL-1 arasındaki dozların hepsinin kontrol gruplarına kıyasla süperoksit dismutaz (SOD) ve katalaz (CAT) aktivitelerini önemli ölçüde azalttığı belirlendi. Çalışmamızın sonuçlarına göre T. capitata ekstrakt yüksek dozları G. mellonella antioksidan savunması üzerinde olumsuz etkilere sahiptir.

Keywords

Malondialdehit, süperoksit dismutaz, katalaz, toplam protein

Project Number

Yok

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