Circadian Rhythm and Leptin Hormone Responses to Nutritional Restriction in Gerbils (Meriones unguiculatus) with Suprachiasmatic Nucleus Lesions

Abstract

Rhythmically-regulated feeding behavior is in harmony with physiologic and metabolic activities in mammals. This rhythmic regulation is orchestrated by Suprachiasmatic Nucleus (SCN). However, it is not entirely clear how the SCN, which generates endogenous (internal) rhythms, influences body weight and serum leptin profile with activity rhythms in relation to feeding timing. In this study, animals in long photoperiod (14L:10D) were divided into two groups as control (sham-SCNx) and SCN lesions (SCNx). Then, these groups were split into four separate subgroups: a) ad libitum feeding; b) feeding only during the dark phase; c) feeding only during the light phase; and d) feeding during a specific period of the day (11:00-14:00 h). Locomotor activity and leptin hormone changes were observed in animals fed in cages attached to activity wheels for one month. Under the conditions of food restriction, the locomotor activities of the groups with SCNx and sham-SCNx demonstrated a phase shift toward the time of feeding. Serum leptin level did not change with feeding conditions but decreased in lesioned groups (SCNx). In conclusion, nutritional restriction caused phase shifts in activity rhythms and it was found that the SCN in gerbils was in charge of these rhythmic changes in the presence and absence of nutrients.

Keywords

• Activity
• body weight
• biological clock
• feeding

Supporting Institution

Directory of Scientific Research Projects of Canakkale Onsekiz Mart University

Project Number

BAP- 2012/051

Ethical Statement

This study was performed in accordance with ethical standards of animal experiments. Legal research ethics committee approval permissions for the study were obtained from the Canakkale Onsekiz Mart University, Animal Experiments Local Ethics Committee (No: 2011/08-03).

Besin Kısıtlaması Uygulanan Suprakiazmatik Nükleus Lezyonlu Gerbillerde (Meriones unguiculatus) Sirkadiyen Ritim ve Leptin Hormon Yanıtları

Abstract

Ritmik olarak düzenlenen beslenme davranışı, memelilerdeki fizyolojik ve metabolik aktivitelerle uyum içindedir. Bu ritmik düzenleme Suprakiyazmatik Nükleus (SCN) tarafından yönetilir. Ancak endojen (iç) ritimler üreten SCN'nin beslenme zamanlamasına bağlı olarak vücut ağırlığını ve serum leptin profilini aktivite ritimleriyle nasıl etkilediği tam olarak açık değildir. Bu çalışmada, uzun fotoperiyottaki (14L:10D) hayvanlar kontrol (sham-SCNx) ve SCN lezyonları (SCNx) olmak üzere iki gruba ayrıldı. Daha sonra bu gruplar a) Ad libitum; b) Sadece gece fazında beslenme; c) Sadece gündüz fazında beslenme ve d) Günün belirli bir zamanında (11:00-14:00) beslenme olmak üzere dört ayrı alt gruba ayrıldı. Bir ay boyunca aktivite tekerleklerine bağlı kafeslerde beslenen hayvanlarda lokomotor aktivite ve leptin hormonu değişiklikleri gözlendi. Besin kısıtlaması koşulları altında, SCNx ve sham-SCNx'li grupların lokomotor aktiviteleri, beslenme zamanına doğru bir faz kayması göstermiştir. Serum leptin düzeyi beslenme koşullarına göre değişmedi ancak lezyonlu gruplarda (SCNx) azalmıştır. Sonuç olarak, beslenme kısıtlamasının aktivite ritimlerinde faz kaymalarına neden olduğu ve gerbillerde SCN'nin besin varlığında ve yokluğunda bu ritmik değişikliklerden sorumlu olduğu belirlendi.

Keywords

• Aktivite
• vücut ağırlığı
• biyolojik saat
• beslenme

Supporting Institution

Çanakkale Onsekiz Mart Üniversitesi Bilimsel Araştırma Projesi

Project Number

BAP- 2012/051

Ethical Statement

Araştırma için yasal etik kurul onayı izinleri Çanakkale Onsekiz Mart Üniversitesi Hayvan Deneyleri Yerel Etik Kurulu'ndan (No: 2011/08-03) alınmıştır.

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