In vitro Antiproliferative Activity of Lissotriton schmidtleri (Raxworthy, 1988) Skin Secretion on Human Breast Cancer (MCF7) Cell Line

Abstract

: In this study, the in vitro antiproliferative activity of Lissotriton schmidtleri skin secretion on estrogen-sensitive human breast cancer (MCF7) cells was reported for the first time. The effects of L. schmidtleri skin secretion at concentrations of 0.5, 5, and 50 μg/mL on the MCF7 cell line were evaluated using the MTT assay after 48 hours of incubation. According to the MTT assay results, L. schmidtleri skin secretion inhibited MCF7 cell viability by approximately 64% at a concentration of 50 μg/mL, with an IC50 value calculated as 20.81 ± 0.87 μg/mL. Based on these findings, it is suggested that L. schmidtleri skin secretion may serve as a potential anticancer agent against breast cancer.

Keywords

• Amphibian skin secretion
• MTT assay
• Lissotriton schmidtleri
• cytotoxicity
• MCF7

Supporting Institution

Ege University

Ethical Statement

This study was performed in accordance with ethical standards of animal experiments. Legal research ethics committee approval permissions for the study were obtained from the Ege University, Animal Experiments Local Ethics Committee (No: 2014-002).

Thanks

I would like to extend my thanks to AREL (Research and Education Laboratory, Ege University School of Medicine) for their laboratory facilities during the study. Also, many thanks to Ayşe NALBANTSOY (Ege University) for her vauable suggestions and providing the cell line.

Lissotriton schmidtleri (Raxworthy, 1988) Deri Salgısının İnsan Meme Kanseri (MCF7) Hücre Hattı Üzerindeki in vitro Antiproliferatif Aktivitesi

Abstract

Bu çalışmada, Lissotriton schmidtleri deri salgısının östrojen duyarlı insan meme kanseri (MCF7) hücreleri üzerindeki in vitro antiproliferatif aktivitesi ilk kez rapor edilmiştir. MCF7 hücre hattına, L. schmidtleri deri salgısının 0.5, 5 ve 50 μg/mL konsantrasyonları uygulanarak 48 saat inkübasyon sonundaki etkileri MTT testi ile belirlenmiştir. MTT testi sonucunda, L. schmidtleri deri salgısı, MCF7 hücre canlılığını 50 μg/mL konsantrasyonda yaklaşık %64 oranında inhibe etmiş ve IC50 değeri 20.81 ± 0.87 μg/mL olarak hesaplanmıştır. Elde edilen veriler ışığında, L. schmidtleri deri salgısının meme kanserine karşı potansiyel bir antikanser ajan olabileceği değerlendirilmiştir.

Keywords

• Amfibi deri salgısı
• MTT testi
• Lissotriton schmidtleri
• sitotoksisite
• MCF7

Supporting Institution

Ege Üniversitesi

Ethical Statement

Bu çalışma hayvan deneyleri etik standartlarına uygun olarak gerçekleştirildi. Çalışma için yasal araştırma etiği kurulu onay izinleri Ege Üniversitesi Hayvan Deneyleri Yerel Etik Kurulu'ndan (No: 2014-002) alındı.

Thanks

Çalışma sırasında laboratuvar olanakları için AREL'e (Ege Üniversitesi Tıp Fakültesi Araştırma ve Eğitim Laboratuvarı) teşekkürlerimi sunmak istiyorum. Ayrıca değerli önerileri ve hücre hattını sağladığı için Ayşe NALBANTSOY'a (Ege Üniversitesi) çok teşekkür ederim.

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