Salvia Cinsi Hemisphace Seksiyonun (Mentheae; Nepetoideae; Lamiaceae) Perikarp İnce Yapısı

Abstract

Lamiaceae familyası içindeki Nepetoideae alt familyasının Mentheae oymağına ait olan Salvia L. (adaçayı) cinsi tıbbi, süs bitkisi, gıda ve halüsinojenik kullanımları nedeniyle ile iyi bilinmektedir. Bu cinsin Hemisphace Benth. seksiyonu Türkiye’de üç tür ile temsil edilir. Bu güncel çalışma, ilgili seksiyona ait morfolojik olarak birbirine benzeyen iki Salvia türü üzerine yürütülmüştür: Salvia napifolia Jacq. ve S. russellii Benth. (S. verticillata L. hariç). Bu amaç için, söz konusu türlerin perikap ince yapısı ışık ve geçirimli elektron mikroskobu (TEM) yardımıyla detaylı olarak araştırılmıştır. Morfometrik karakterler çoklu karşılaştırmalar için tek yönlü Varyans Analizi (ANOVA) ile Tukey’in tam olarak önemli fark (HSD) post-hoc testi kullanılarak analiz edilmiştir. Perikarp özelliklerinin taksonomik önemi tartışılmıştır. Çalışılan türlerin ayrımına izin veren en önemli özellikler; perikarp, mesokarp ve sklerenkima bölgesinin kalınlığıdır. Her iki türün ıslatılmış merikarplarının yüzeyinde miksokarpi (müsilaj oluşumu) görülmüştür. Müsilajlı hücreler orta düzeyde bir reaksiyon göstermiştir, fakat S. napifolia’daki müsilajlı tabaka biraz daha kalındır (yaklaşık 0.20-0.34 mm). Bu çalışma incelenen türlerin perikarp ince yapısı üzerine ilk detaylı bulguları sunmaktadır.

Keywords

• Lamiaceae
• perikarp ince yapısı
• Salvia
• Hemisphace seksiyonu
• TEM

Pericarp Ultrastructure of Salvia Section Hemisphace (Mentheae; Nepetoideae; Lamiaceae)

Abstract

The genus Salvia L. (sage), which belongs to the tribe Mentheae of the subfamily Nepetoideae within the family Lamiaceae, is well-known for its medicinal, ornamental, culinary and hallucinogenic uses. The section Hemisphace Benth. of this genus is respresented in Turkey by three species. The present study is conducted on two morphologically similar Salvia species belonging to this section: Salvia napifolia Jacq. and S. russellii Benth. (excluding S. verticillata L.). For this purpose, the pericarp ultrastructure of these species is investigated in detail with the help of light and transmission electron microscopy (TEM). Morphometric characters are analyzed using one-way Analysis of Variance (ANOVA) with Tukey’s honestly significant difference (HSD) post-hoc test for multiple comparisons. The taxonomic potential of pericarp characteristics is discussed. The most prominent traits are the thickness of the pericarp, mesocarp and sclerenchyma region that permit the separation of the species studied. Myxocarpy (mucilage formation) is recognized on the surface of the wetted mericarps of both species. Mucilaginous cells reveal a moderate reaction but S. napifolia is of a somewhat thicker mucilaginous layer, about 0.20-0.34 mm. This study represents the first comprehensive findings on the pericarp ultrastructure of the species examined.

Keywords

• Lamiaceae
• pericarp ultrastructure
• Salvia
• section Hemisphace
• TEM
• Turkey

References

1. Baytop, T. (1999). Therapy with Medicinal Plants in Turkey (Past and Present). İstanbul, Turkey, Nobel Medical Presshouse, 480 pp.
2. Bilimovitsch, O. (1935). On the structure of the pericarp in the Labiatae from a systematic viewpoint. Trudy Voronezhsk. Gosud. Univ., 7, 68-84.
3. Boissier, E. (1879). Flora Orientalis, vol. 4. Bassel & Genéve, 1276 pp.
4. Budantsev, A.L. (1993a). Ultrastructural features of fruit surface in genus Nepeta (Lamiaceae). Botanicheskii Zhurnal, 78(4), 80-87.
5. Budantsev, A.L. (1993b). The ultrastructural features of fruit surface in some genera of the tribe Nepeteae (Lamiaceae). Botanicheskii Zhurnal, 78(5), 100-108.
6. Budantsev, A.V., & Lobova, T.A. (1997). Fruit morphology, anatomy and taxonomy of tribe Nepeteae (Labiatae). Edinburgh Journal of Botany, 54(2), 183-216. https://doi.org/10.1017/S0960428600004029
7. Büyükkartal, H.N., Kahraman, A., Çölgeçen, H., Doğan, M., & Karabacak, E. (2011). Mericarp micromorphology and anatomy of Salvia hedgeana Dönmez, S. huberi Hedge and S. rosifolia Sm. (section Salvia Hedge, Lamiaceae). Acta Botanica Croatica, 70(1), 65-80. https://doi.org/10.2478/v10184-010-0011-8
8. Davis, P.H., & Heywood, V.H. (1973). Principles of Angiosperm Taxonomy. Huntington, New York, Robert E. Kieger Publishing Co., 558 pp.

9. Doğan, M., Akaydın, G., Celep, F., Bagherpour, S., Kahraman, A., & Karabacak, E. (2007). Infrageneric delimitation of Salvia L. (Labiatae) in Turkey. Proceedings 7th International Symposium on Plant Life of Southwest Asia. Eskişehir, Turkey.
10. Duletić-Lauševıc, S., & Marin, P.D. (1999). Pericarp structure and myxocarpy in selected genera of Nepetoideae (Lamiaceae). Nordic Journal of Botany, 19(4), 435-446. https://doi.org/10.1111/j.1756-1051.1999.tb01227.x
11. Er, M., Tugay, O., Özcan, M.M., Ulukuş, D., & Al-Juhaimi, F. (2013). Biochemical properties of some Salvia L. species. Environmental Monitoring and Assessment, 185(6), 5193-5198. https://doi.org/10.1007/s10661-012-2935-z
12. Guerin, G.R. (2005). Nutlet morphology in Hemigenia R. Br. and Microcorys R. Br. (Lamiaceae). Plant Systematics and Evolution, 254(1-2), 49-68. https://doi.org/10.1007/s00606-005-0311-y
13. Harley, R. M., Atkins, S., Budantsev, A., Cantino, P.D., Conn, B.J., Grayer, R., Harley, M.M., ... Upson, T. (2004). Labiatae. In K. Kubitzki, (Ed.), The Families and Genera of Vascular Plants, vol. 7 (pp. 167-275). Heidelberg, Berlin, Springer-Verlag.
14. Hassan, S.A., & Al. Thobaiti, A.T. (2014). II. Pericarp anatomical study of some Lamiaceae nutlets in Saudi Arabia and its taxonomic significance. Pakistan Journal of Botany, 46(5), 1643-1651.
15. Hedge, I.C., Lamond, J. (1968). Studies in the flora of Afghanistan: VII, Labiatae. Notes from the Royal Botanic Garden Edinburgh, 28, 97-123.
16. Hedge, I.C. (1970). Observations on the mucilage of Salvia fruits. Notes from the Royal Botanic Garden Edinburgh, 30, 79-95.
17. Hedge, I.C. (1982). Flora of Turkey and the East Aegean Islands. In P.H. Davis, (Ed.), Salvia L., vol. 7 (pp. 400-461). Edinburgh, UK, Edinburgh University Press, 947 pp.
18. Kirmizibekmez, H., Bardakci Altan, H., Liktor-Busa, E., Zana, A., Yesilada, E., & Hohmann, J. (2012). Chemical constituents of Salvia dichroantha. Biochemical Systematics and Ecology, 42, 18-20. https://doi.org/10.1016/j.bse.2012.01.001
19. Kotan, R., Kordali, S., Cakir, A., Kesdek, M., Kaya, Y., & Kilic, H. (2008). Antimicrobial and insecticidal activities of essential oil isolated from Turkish Salvia hydrangea DC. ex Benth. Biochemical Systematics and Ecology, 36(5-6), 360-368. https://doi.org/10.1016/j.bse.2007.12.003
20. Luft, J.H. (1961). Improvements in epoxy resin embedding methods. Journal of Biophysical and Biochemical Cytology, 9(2), 409-414. https://doi.org/10.1083/jcb.9.2.409
21. Makarova, Z.I. (1967). On the significance of the anatomical structure of the pericarp for the taxonomy of the tribe Nepeteae. Botanicheskii Zhurnal, 52, 33-41.
22. Moon, H.K., & Hong, S.P. (2006). Nutlet morphology and anatomy of the genus Lycopus (Lamiaceae. Mentheae). Journal of Plant Research, 119(6), 633-644. https://doi.org/10.1007/s10265-006-0023-6
23. Mosquero, M.A.M., Juan, R., Pastor, J.E. (2002). Morphological and anatomical studies on nutlets of Nepeta L. (Lamiaceae) from South-West Spain. Acta Botanica Malacitana, 27, 15-25.
24. Oran, S.A. (1997). Nutlet anatomy of the genus Salvia L. in Jordan. Flora Mediterranea, 7, 27-40.
25. Ryding, O. (1992a). The distribution and evolution of myxocarpy in Lamiaceae. In R.M., Harley, & T. Reynolds, T. (Eds.), Advances in Labiatae Science (pp. 85-96). Royal Botanic Gardens, Kew, UK.
26. Ryding, O. (1992b). Pericarp structure and phylogeny within Lamiaceae subfamily Nepetoideae tribe Ocimeae. Nordic Journal of Botany, 12(3), 273-298. https://doi.org/10.1111/j.1756-1051.1992.tb01304.x
27. Ryding, O. (1993a). Pericarp structure and systematic positions of five genera of Lamiaceae subg. Nepetoideae tribe Ocimeae. Nordic Journal of Botany, 13(6), 631-635. https://doi.org/10.1111/j.1756-1051.1993.tb00107.x
28. Ryding, O. (1993b). Pericarp structure of Leucas and related genera (Lamiaceae subfam. Lamioideae). Nordic Journal of Botany, 13(6), 637-646. https://doi.org/10.1111/j.1756-1051.1993.tb00108.x
29. Ryding, O. (1995). Pericarp structure and phylogeny of the Lamiaceae-Verbenaceae-complex. Plant Systematics and Evolution, 198(1-2), 101-141. https://doi.org/10.1007/bf00985109
30. Ryding, O. (2001). Myxocarpy in the Nepetoideae (Lamiaceae) with notes on myxodiaspory in general. Systematics and Geography of Plants, 71(2), 503-514. https://doi.org/10.2307/3668696
31. Ryding, O. (2010). Pericarp structure and phylogeny of tribe Mentheae (Lamiaceae). Plant Systematics and Evolution, 285(3-4), 165-175. https://doi.org/10.1007/s00606-010-0270-9
32. Sato, J.E. (1968). A modified method for lead staining of thin sections. Journal of Electron Microscopy, 17(2), 158-159. https://doi.org/10.1093/oxfordjournals.jmicro.a049610
33. Sokal, R., & Rohlf, F.J. (1995). Biometry: The Principles and Practice of Statistics in Biological Research. New York, W.H. Freeman & Company, 887 pp.
34. Stempak, J.G., & Ward, W.P. (1964). An improved staining method for electronmicroscopy. Journal of Cell Biology, 22(3), 697-701. https://doi.org/ 10.1083/jcb.22.3.697
35. Swarbrick, J.T. (1971). External mucilage production by seeds of British plants. Botanical Journal of the Linnean Society, 64(2), 157-162. https://doi.org/10.1111/j.1095-8339.1971.tb02142.x
36. Topcu, G., Turkmen, Z., Schilling, J.K., Kingston, D.G. I., Pezzuto, J.M., & Ulubelen, A. (2008) Cytotoxic activity of some Anatolian Salvia extracts and isolated abietane diterpenoids. Pharmaceutical Biology, 46(3): 180-184. https://doi.org/10.1080/13880200701735411
37. Ulubelen, A. (2003). Cardioactive and antibacterial terpenoids from some Salvia species. Phytochemistry, 64(2), 395-399. https://doi.org/10.1016/s0031-9422(03)00225-5
38. Wagner, S. (1914). Contribution à l’étude anatomique du fruit des Labiées. (PhD Thesis). Université de Paris, Lons-le-Saunier, France.
39. Walker, J.B., & Sytsma, K.J. (2007). Staminal evolution in the genus Salvia (Lamiaceae): molecular phylogenetic evidence for multiple origins of the staminal lever. Annals of Botany, 100(2), 375-391. https://doi.org/10.1093/aob/mcl176
40. Witztum, A. (1978). Mucilaginous plate cells in the nutlet epidermis of Coleus blumei Benth. (Labiatae). Botanical Gazette, 139(4), 430-435. https://doi.org/10.1086/337018
41. Wojciechowska, B. (1958). Taxonomy, morphology and anatomy of fruits and seeds in the genus Salvia L. Monographiae Botanicae, 6, 3-55.
42. Wojciechowska, B. (1961a). Fruits of the Middle European species of Prunella L. (fam. Labiatae), theier morphology and anatomy. Monographiae Botanicae, 12, 49-88.
43. Wojciechowska, B. (1961b). Fruits in the Middle European species of some genera of Stachyoideae (fam. Labiatae). Monographiae Botanicae, 12, 89-120.
44. Wojciechowska, B. (1966). Morphology and anatomy of fruits and seeds in the family Labiatae with particular respect to medicinal species. Monographiae Botanicae, 21, 3-244.
45. Wojciechowska, B. (1972). Fruit morphology and anatomy inScutellaria, Chaiturus, Galeobdolon and Sideritis of the family Labiatae. Monographiae Botanicae, 37, 137-169.