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Adıyaman'da Yetişen Endemik Serratula olygocephala’nın Moleküler Karakterizasyonu ve Bazı Biyokimyasal Özelliklerini Belirlenmesi

Year 2019, , 32 - 36, 30.06.2019
https://doi.org/10.31594/commagene.559626

Abstract

Bu çalışmada Adıyaman'da
Yetişen Endemik Serratula olygocephala’nın
moleküler karakterizasyonu yapıldı. DNA izolasyonu Plant DNeasy (Qiagen Inc.)
kiti kullanılarak, kit protokolü takip edilerek yapıldı. Bunun yanında bitkinin
antioksidan aktivitesi ve yağ asidi kompozisyonu da belirlenmiştir. Antioksidan
etkinliği bakımından, bitkinin toplam fenolik madde konsantrasyonu folin
yöntemiyle belirlendi ve serbest radikal süpürme kapasitesini ise invitro olarak DPPH radikal süpürme
testi ile tayin edildi. Serratula
olygocephala’
nın yağ asidi kompozisyonu ise gaz kromatografi yöntemiyle
tayin edildi. Doymuş, tekli doymamış ve çoklu doymamış yağ asitleri yüzdesi
belirlenmiştir.

Supporting Institution

Adıyaman Üniversitesi Bilimsel Araştırma Projeleri Birim (ADYÜ-BAP)

Project Number

ECZFMAP/2016-0001

References

  • Başaran, A. (2005). Fitoterapi, nasıl ve nereye kadar? Ankara Eczacı Odası Bizim Gazete, 7.
  • Bathori, M., Zupko, I., Hunyadi, A., Gacsne-Baitz, E., Dinya, Z., & Forgo, P. (2004). Monitoring the antioxidant activity of extracts originated from various Serratula species and isolation of flavonoids from Serratula coronate. Fitoterapia, 75(2), 162-167.
  • Blainski, A., Lopes, G.C., & Mello, J.C.P. (2013). Application and Analysis of the Folin Ciocalteu Method for the Determination of the Total Phenolic Content from Limonium brasiliense L. Molecules, 18, 6852-6865.
  • Christie, W.W. (1992). Gas Chromatography and Lipids. The Oil Press, Glaskow, 302 pp.
  • Davis, P. H. (ed.) 1965-1988: Flora of Turkey and the East Aegean Islands 1-9, (7), - Edinburgh University Press, Edinburgh, 417 pp.
  • Hall, T.A. (1999). Bioedit: A User-Friendly Biological Sequence Alignment Editor and Analyses Program for Windows 95/98/Nt, Nucleic Acids Symposium Series, 41, 95-98.
  • Hara, A., & Radin, N.S. (1978). Lipid extraction of tissues with a low-toxicity solvent. Analitical Biochemistry, 901, 420-426.
  • Masaki, H., Sakaki, S., Atsumi, T., & Sakurai, H. (1995). Active-oxygen scavenging activity of plant extracts. Biological and Pharmaceutical Bulletin, 18, 162–166.
  • Özkaya, A., Sucak, M.G., Ağyar, O., Yılmaz, E. (2018). Determination of Mineral and Fatty Acid Concentrations of Akkaraman Sheep’s Milk, Commagene Journal of Biology, 2(2), 1-4.
  • Özkaya, A., Tel, A.Z., Gökler, I., Çiftçi., H., & Han, U.Y. (2017). Determination of fatty acids and elements in Allium nemrutdaghense Kit Tan & F. Sorger endemic plant. Biharean Biologist, 11(1), 1-4.
  • Pietta, P.G. (1998). Flavonoids in medicinal plants. In: Rice-Evans CA, Packer L (eds) Flavonoids in health and disease (pp 61–110). Dekker, New York, USA.
  • Şahin, N. (2011). Türkiye’de Yetişen Serratula L. (Asteraceae) Cinsine Ait Taksonların Its Nrdna Ve Trnl-F Cpdna Dizileriyle Moleküler Sistematik Analizi (Yüksek Lisans Tezi). Balıkesir Üniversitesi, Fen Bilimleri Enstitüsü, Balıkesir, Türkiye.
  • Sanchez-Moreno, C., Larrauri, J.A., & Saura-Calixto, F., (1998). A procedure to measure the antiradical efficiency of polyphenols. Journal of Science Food Agriculture, 76, 270-276.
  • Shahidi, F., Janitha, P., & Wanasundara, .P.D. (1992). Phenolic antioxidants. Critical Reviews in Food Science and Nutrition, 32, 67–103.
  • Swofford, D.L. (2002). Paup. Phylogenetic Analysis Using Parsimony (and Other Methods), Version 4, Sinauer.
  • Tekeli, Y., & Sezgin, M. (2007). Centaurea Carduiformis (Peygamber Çiçeği)’in Antioksidan Aktivitesinin Belirlenmesi, SDÜ Fen Edebiyat Fakültesi Fen Dergisi, 2(2), 204-209.
  • Tekeli, Y., (2008). Konya Bölgesindeki Bazı Centaurea Türlerinin Bazı Kimyasal ve Biyolojik Özelliklerinin Belirlenmesi (Doktora tezi). Selçuk Üniversitesi, Konya, Türkiye.

Molecular Characterization and Determination of Some Biochemical Properties of Endemic Serratula olygocephala Growing in Adıyaman

Year 2019, , 32 - 36, 30.06.2019
https://doi.org/10.31594/commagene.559626

Abstract

In
this study, molecular characterization of endemic Serratula olygocephala grown in Adiyaman was determined. DNA
isolation was performed using the Plant DNeasy (Qiagen Inc.) kit, following the
kit protocol. In addition, the antioxidant activity and fatty acid composition
of the plant were also determined. In terms of the antioxidant activity, total
phenolic substance concentration of the plant was determined by applying the
folin method and free radical scavenging capacity was determined by DPPH
radical scavenging test. Fatty acid profile of Serratula oligocephala was determined by gas chromatography. The
percentage of the saturated, monounsaturated, and polyunsaturated fatty acids
was calculated.

Project Number

ECZFMAP/2016-0001

References

  • Başaran, A. (2005). Fitoterapi, nasıl ve nereye kadar? Ankara Eczacı Odası Bizim Gazete, 7.
  • Bathori, M., Zupko, I., Hunyadi, A., Gacsne-Baitz, E., Dinya, Z., & Forgo, P. (2004). Monitoring the antioxidant activity of extracts originated from various Serratula species and isolation of flavonoids from Serratula coronate. Fitoterapia, 75(2), 162-167.
  • Blainski, A., Lopes, G.C., & Mello, J.C.P. (2013). Application and Analysis of the Folin Ciocalteu Method for the Determination of the Total Phenolic Content from Limonium brasiliense L. Molecules, 18, 6852-6865.
  • Christie, W.W. (1992). Gas Chromatography and Lipids. The Oil Press, Glaskow, 302 pp.
  • Davis, P. H. (ed.) 1965-1988: Flora of Turkey and the East Aegean Islands 1-9, (7), - Edinburgh University Press, Edinburgh, 417 pp.
  • Hall, T.A. (1999). Bioedit: A User-Friendly Biological Sequence Alignment Editor and Analyses Program for Windows 95/98/Nt, Nucleic Acids Symposium Series, 41, 95-98.
  • Hara, A., & Radin, N.S. (1978). Lipid extraction of tissues with a low-toxicity solvent. Analitical Biochemistry, 901, 420-426.
  • Masaki, H., Sakaki, S., Atsumi, T., & Sakurai, H. (1995). Active-oxygen scavenging activity of plant extracts. Biological and Pharmaceutical Bulletin, 18, 162–166.
  • Özkaya, A., Sucak, M.G., Ağyar, O., Yılmaz, E. (2018). Determination of Mineral and Fatty Acid Concentrations of Akkaraman Sheep’s Milk, Commagene Journal of Biology, 2(2), 1-4.
  • Özkaya, A., Tel, A.Z., Gökler, I., Çiftçi., H., & Han, U.Y. (2017). Determination of fatty acids and elements in Allium nemrutdaghense Kit Tan & F. Sorger endemic plant. Biharean Biologist, 11(1), 1-4.
  • Pietta, P.G. (1998). Flavonoids in medicinal plants. In: Rice-Evans CA, Packer L (eds) Flavonoids in health and disease (pp 61–110). Dekker, New York, USA.
  • Şahin, N. (2011). Türkiye’de Yetişen Serratula L. (Asteraceae) Cinsine Ait Taksonların Its Nrdna Ve Trnl-F Cpdna Dizileriyle Moleküler Sistematik Analizi (Yüksek Lisans Tezi). Balıkesir Üniversitesi, Fen Bilimleri Enstitüsü, Balıkesir, Türkiye.
  • Sanchez-Moreno, C., Larrauri, J.A., & Saura-Calixto, F., (1998). A procedure to measure the antiradical efficiency of polyphenols. Journal of Science Food Agriculture, 76, 270-276.
  • Shahidi, F., Janitha, P., & Wanasundara, .P.D. (1992). Phenolic antioxidants. Critical Reviews in Food Science and Nutrition, 32, 67–103.
  • Swofford, D.L. (2002). Paup. Phylogenetic Analysis Using Parsimony (and Other Methods), Version 4, Sinauer.
  • Tekeli, Y., & Sezgin, M. (2007). Centaurea Carduiformis (Peygamber Çiçeği)’in Antioksidan Aktivitesinin Belirlenmesi, SDÜ Fen Edebiyat Fakültesi Fen Dergisi, 2(2), 204-209.
  • Tekeli, Y., (2008). Konya Bölgesindeki Bazı Centaurea Türlerinin Bazı Kimyasal ve Biyolojik Özelliklerinin Belirlenmesi (Doktora tezi). Selçuk Üniversitesi, Konya, Türkiye.
There are 17 citations in total.

Details

Primary Language Turkish
Subjects Structural Biology
Journal Section Research Articles
Authors

Yener Tekeli 0000-0003-1524-457X

Ahmet Özkaya 0000-0002-0173-3084

Ahmet Zafer Tel This is me 0000-0002-1204-3839

Görkem Deniz Sönmez 0000-0002-3613-0195

Süleyman Akocak 0000-0003-4506-5265

Project Number ECZFMAP/2016-0001
Publication Date June 30, 2019
Submission Date May 2, 2019
Acceptance Date May 20, 2019
Published in Issue Year 2019

Cite

APA Tekeli, Y., Özkaya, A., Tel, A. Z., Sönmez, G. D., et al. (2019). Adıyaman’da Yetişen Endemik Serratula olygocephala’nın Moleküler Karakterizasyonu ve Bazı Biyokimyasal Özelliklerini Belirlenmesi. Commagene Journal of Biology, 3(1), 32-36. https://doi.org/10.31594/commagene.559626
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