Sıçanların Epididimis ve Dalak Dokularında Kurşun ve Kadmiyuma Bağlı Oksidatif Strese Karşı Sesamolün Etkileri

Abstract

Kurşun ve kadmiyum, hayvan ve insan sağlığına zarar veren gıda ve su kirleticilerinde yaygın olarak bulunan bilinen çevre kirleticileridir. Sesamol, bazı bitkilerde bulunan bir diyet antioksidanıdır. Epididimisin spermin olgunlaşması ve depolanmasında önemli bir rol oynadığı bilinmektedir. Dalak, bağışıklık tepkisinde rol oynayan önemli bir organdır. Bu çalışma, dalak ve epididimdeki oksidatif stresi analiz etmeyi amaçlamaktadır. Bu amaçla sıçanlara kurşun (günde 90 mg/kg canlı ağırlık, 1/25 LD50), kadmiyum (günde 3 mg/kg canlı ağırlık, 1/25 LD50) ve sesamol (günde 50 mg/kg canlı ağırlık) verildi. 28 gün boyunca gavaj, 28 gün sonunda epididimis ve dalak dokularındaki antioksidan enzim aktiviteleri [süperoksit dismutaz (SOD), katalaz (CAT), glutasyon peroksidaz (GPx) ve glutatyon S transferaz], malondialdehit (MDA) kontrol grubu ile karşılaştırmalı olarak araştırıldı. Kontrol grubuna göre kurşun ve kadmiyum uygulanan sıçanların dalak ve epididimis dokularında antioksidan enzimlerinde istatistiksel olarak anlamlı bir azalma ve MDA düzeylerinde artış olmuştur. Bu parametrelerde sesamol ile tedavi edilen gruplarda daha az artış gözlenmiştir.

Keywords

• Antioksidanlar
• ağır metal,
• lipid peroksidasyonu,
• organ toksisitesi

Lead and Cadmium Induced Oxidative Stress in the Epididymis and Spleen of Rats: Effects of Sesamol

Abstract

Lead and cadmium are known as environmental pollutants extensively found in food and water that induce hazards to animals’ and people’s health. Sesamol is a dietary antioxidant that is found in some plants. Epididymis is known to play an important role in the maturation and storage of the sperm. The spleen is an important organ involved in the immune response. The present study aims to analyze the oxidative stress in spleen and epididymis. Therefore, Lead (LN) (90 mg/kg bw per day, 1/25 LD50), Cadmium (CdCl2) (3 mg/kg bw per day, 1/25 LD50), and sesamol (50 mg/kg bw per day) were given to rats by gavage for 28 days. Antioxidant enzyme activities in epididymis and spleen tissues [superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione S transferase], malondialdehyde (MDA) were investigated at the end of 28 days comparatively with the control group. It is revealed that there is a significant decline in the antioxidant enzymes and increase in MDA levels in the spleen and epididymis tissues of the lead and cadmium treated rats compared to the control group. A small increase in these parameters was also observed in the sesamol treated groups.

Keywords

• Antioxidants
• Heavy metals
• Lipid peroxidation
• organ toxicity

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