Chromosome Analysis of Micaria formicaria (Araneae: Gnaphosidae) from Turkey

Year 2021, , 35 - 38, 30.06.2021

Ümit Kumbıçak , Zübeyde Kumbıçak , Fahrettin Anıl Sırlıbaş , Şeyma Civan , Hatice Poyraz

https://doi.org/10.31594/commagene.862337

Abstract

In this study, Micaria formicaria (Sundevall, 1831) karyotype properties of the species were analyzed for the first time by using standard giemsa staining method. Gonads derived from the testicles were put through hypotonic application and, fixation and staining processes then, chromosome preparations were prepared. In this study, the number of diploid chromosomes belonging to the species was determined as 2n=22. All chromosomes including sex chromosomes are telocentric type; the sexual chromosome system was determined as X1X20. In the meiosis I phases, 10 autosomal bivalent and two heteropicnotic sexual chromosomes with at least one chiasm point were observed. In meiosis II phases, sexual chromosomes showed an isopicnotic feature.

Keywords

Spider, karyotypes, cytogenetic, meiosis

Türkiye’den Micaria formicaria (Araneae: Gnaphosidae) Türünün Kromozom Analizi

Abstract

Bu çalışmada Micaria formicaria (Sundevall, 1831) türünün karyotip özellikleri, standart giemsa boyama yöntemi kullanılarak ilk kez analiz edilmiştir. Testislerden elde edilen gonadlar hipotonik uygulama, fiksasyon ve boyama işlemlerinden geçirilerek kromozom preparatları hazırlanmıştır. Çalışmada türe ait diploid kromozom sayısı 2n=22 olarak belirlenmiştir. Eşey kromozomları da dâhil tüm kromozomlar telosentrik tipte olup eşey kromozom sistemi X1X20 olarak tespit edilmiştir. Mayoz I evrelerinde en az bir kiyazma noktası bulunan 10 otozomal bivalent ve heteropiknotik özellikte iki eşey kromozomu görülmüştür. Mayoz II evrelerinde ise eşey kromozomları izopiknotik özellik göstermiştir.

Keywords

Örümcek, karyotip, sitogenetik, mayoz

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