Abstract

Şalak kayısı çeşidi (Prunus armeniaca cv. Şalak) Aras Havzası’nda üretimi yapılan ve coğrafi işarete sahip bir kayısı çeşididir. Tescilli ismi Iğdır Kayısısı olarak belirlenmiştir. Özellikle gıda aldatmacasına maruz kalma potansiyeli olan özel gıda ürünlerinin DNA temelli yöntemler ile tanımlanması güvenilir ve görece ucuz maliyetlidir. Bu çalışmanın amacı Şalak kayısı çeşidinin, yakın ilişkili kayısı çeşitlerden Yüksek Çözünürlüklü Erime (HRM) yöntemi kullanarak ayırt edilmesi için bir protokol geliştirmektir. Çalışmada, daha önceden Prunus türleri için doğrulanmış 12 adet SSR belirtecinin, Türkiye’de üretimi yapılan Şalak (AS), Hasanbey (HB), Hacıhaliloğlu (HH), Kabaaşı (KB) ve Şekerpare (SK) kayısı çeşitlerini ayırt etme başarısı test edilmiştir. Çalışmada ayrıca HRM verilerinden Temel Bileşenler Analizi yapılabilmesi için R yazılımında kullanılabilecek bir komut dosyası oluşturulmuştur. Kılcal elektroforez ile doğrulanmış 12 SSR belirtecinden 11 tanesinin, her kayısı çeşidi için farklı fragmentler çoğalttığı tespit edilmiştir. Temel Bileşenler Analizi ile desteklenmiş HRM sonuçlarının Şalak kayısı çeşidini diğer çeşitlerden başarılı bir şekilde ayırt ettiği belirlenmiştir.

Keywords

• Genotipleme
• mikrosatelitler
• genetik doğrulama
• gıda aldatmacası
• SSR-HRM

Distinguishing the Protected Designation of Origin Apricot (Prunus armeniaca L. cv. Şalak) from Closely Related Cultivars by High Resolution Melting

Abstract

The apricot cultivar Prunus armeniaca cv. Şalak (registered as “Iğdır Kayısısı”) is a Protected Designation of Origin (PDO) apricot and produced in Aras Basin (Iğdır, Turkey) region. Authenticating the special products, which has adulteration potential, by DNA based methods is reliable and cost-effective. The aim of the current study is to distinguish the PDO apricot from closely related cultivars by High Resolution Melting. We tested 12 SSR markers previously validated for Prunus spp. by means of distinguishing the ability of five closely related apricot cultivars that are Şalak (AS), Hasanbey (HB), Hacıhaliloğlu (HH), Kabaaşı (KB), and Şekerpare (SK) produced in Turkey. Capillary electrophoresis validation showed 11 of 12 markers amplified unique fragments for the cultivars. HRM analysis combined with the Principal Component Analysis (PCA) successfully distinguished the PDO AS from closely related cultivars. HRM analysis combined with PCA can be a cost-effective and reliable authenticating method for PDO food products.

Keywords

• Genotyping
• Microsatellites
• Authenticity
• Food adulteration
• SSR-HRM

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