Year 2020, Volume 4 , Issue 1, Pages 62 - 66 2020-06-29

The Effect of Salinity on Growth, Antagonistic Potential, Protease Activity, and Proline Content of Trichoderma harzianum
Tuzluluğun Trichoderma harzianum'un Büyüme, Antagonistik Potansiyeli, Proteaz Aktivitesi ve Prolin İçeriği Üzerindeki Etkisi

Sedat ÇAM [1] , Çiğdem KÜÇÜK [2]


Salinity is one of the major limiting factors for sustainable crop production and the growth of beneficial microorganisms. Screening of biocontrol agent Trichoderma harzianum isolates under saline conditions could be necessary to select more efficient strains to alleviate salt stress through biopriming in plants. The present study aimed to investigate the antagonistic capacity of 14 T. harzianum isolates against Macrophomina phaseolina as well as the growth, protease activity, and proline content under different concentrations of salt. All isolates except Th15 and Th18 exhibited an over 85% inhibition against M. phaseolina at 0 mM NaCl in c and showed a noticeable decline in their antagonistic capacity as salt concentration increased. Colony growth decreased with increasing salt concentrations in all isolates tested. The growth of all isolates at 0 mM was significantly higher than the other NaCl treatments except at 70 mM NaCl. Protease activity also declined with the increased level of salt. Isolates displayed a wide range of protease expression patterns even at the same salinity as in the case of proline content. Unlike protease enzyme, proline content significantly increased at 240 mM NaCl. Salinity played a significant role in T. harzianum isolates with regards to the growth, antagonistic capacity, protease activity, and proline content.

Sürdürülebilir bitkisel üretimin artması ve faydalı mikroorganizmaların büyümesi için tuzluluk ana sınırlayıcı faktörlerden biridir. Biyokontrol ajanı olan Trichoderma harzianum izolatlarının tuzlu koşullar altında test edilmesi, biyo-priming yoluyla bitkilerdeki tuz stresini hafifletmek için daha uygun suşlar seçmek gereklidir. Bu çalışmada, 14 T. harzianum izolatının Macrophomina phaseolina’ ya karşı antagonistik kapasitesinin yanı sıra büyüme, proteaz aktivitesi ve prolin içeriği farklı tuz konsantrasyonları altında araştırıldı. Th15 ve Th18 dışındaki tüm izolatlar, ikili kültür analizinde 0 mM NaCl'de M. phaseolina'ya karşı % 85'in üzerinde bir inhibisyon sergiledi ve tuz konsantrasyonu arttıkça antagonistik kapasitelerinde belirgin bir düşüş gösterdi. Test edilen tüm izolatlarda artan tuz konsantrasyonlarına bağlı olarak koloni büyümelerinde istatistiksel açıdan anlamlı azalmalar gözlemlendi. Tüm izolatların 0 mM'deki büyümesi, 70 mM NaCl hariç diğer NaCl uygulamalarında büyük ölçüde daha fazlaydı. Artan tuz seviyesi ile proteaz aktivitesi de azalmıştır. İzolatlar, prolin içeriğinde olduğu gibi aynı tuzlulukta bile farklı miktarlarda proteaz üretimi gösterdiler. Proteaz enziminin aksine, prolin içeriği 240 mM NaCl'de önemli ölçüde artmıştır. Tuzluluk, T. harzianum izolatlarında büyüme, antagonistik kapasitesi, proteaz aktivitesi ve prolin içeriği bakımından önemli bir rol oynamıştır.

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Primary Language en
Subjects Biology
Journal Section Research Articles
Authors

Orcid: 0000-0001-9030-6713
Author: Sedat ÇAM (Primary Author)
Institution: Harran Üniversitesi
Country: Turkey


Orcid: 0000-0001-5688-5440
Author: Çiğdem KÜÇÜK
Institution: Harran Ünversitesi
Country: Turkey


Dates

Application Date : May 16, 2020
Acceptance Date : June 16, 2020
Publication Date : June 29, 2020

Bibtex @research article { commagene738313, journal = {Commagene Journal of Biology}, issn = {}, eissn = {2602-456X}, address = {Adıyaman Bilimsel Araştırmalar Derneği,}, publisher = {ABADER (Adıyaman Bilimsel Araştırmalar Derneği)}, year = {2020}, volume = {4}, pages = {62 - 66}, doi = {10.31594/commagene.738313}, title = {The Effect of Salinity on Growth, Antagonistic Potential, Protease Activity, and Proline Content of Trichoderma harzianum}, key = {cite}, author = {Çam, Sedat and Küçük, Çiğdem} }
APA Çam, S , Küçük, Ç . (2020). The Effect of Salinity on Growth, Antagonistic Potential, Protease Activity, and Proline Content of Trichoderma harzianum . Commagene Journal of Biology , 4 (1) , 62-66 . DOI: 10.31594/commagene.738313
MLA Çam, S , Küçük, Ç . "The Effect of Salinity on Growth, Antagonistic Potential, Protease Activity, and Proline Content of Trichoderma harzianum" . Commagene Journal of Biology 4 (2020 ): 62-66 <https://dergipark.org.tr/en/pub/commagene/issue/52124/738313>
Chicago Çam, S , Küçük, Ç . "The Effect of Salinity on Growth, Antagonistic Potential, Protease Activity, and Proline Content of Trichoderma harzianum". Commagene Journal of Biology 4 (2020 ): 62-66
RIS TY - JOUR T1 - The Effect of Salinity on Growth, Antagonistic Potential, Protease Activity, and Proline Content of Trichoderma harzianum AU - Sedat Çam , Çiğdem Küçük Y1 - 2020 PY - 2020 N1 - doi: 10.31594/commagene.738313 DO - 10.31594/commagene.738313 T2 - Commagene Journal of Biology JF - Journal JO - JOR SP - 62 EP - 66 VL - 4 IS - 1 SN - -2602-456X M3 - doi: 10.31594/commagene.738313 UR - https://doi.org/10.31594/commagene.738313 Y2 - 2020 ER -
EndNote %0 Kommagene Biyoloji Dergisi The Effect of Salinity on Growth, Antagonistic Potential, Protease Activity, and Proline Content of Trichoderma harzianum %A Sedat Çam , Çiğdem Küçük %T The Effect of Salinity on Growth, Antagonistic Potential, Protease Activity, and Proline Content of Trichoderma harzianum %D 2020 %J Commagene Journal of Biology %P -2602-456X %V 4 %N 1 %R doi: 10.31594/commagene.738313 %U 10.31594/commagene.738313
ISNAD Çam, Sedat , Küçük, Çiğdem . "The Effect of Salinity on Growth, Antagonistic Potential, Protease Activity, and Proline Content of Trichoderma harzianum". Commagene Journal of Biology 4 / 1 (June 2020): 62-66 . https://doi.org/10.31594/commagene.738313
AMA Çam S , Küçük Ç . The Effect of Salinity on Growth, Antagonistic Potential, Protease Activity, and Proline Content of Trichoderma harzianum. Comm. J. Biol.. 2020; 4(1): 62-66.
Vancouver Çam S , Küçük Ç . The Effect of Salinity on Growth, Antagonistic Potential, Protease Activity, and Proline Content of Trichoderma harzianum. Commagene Journal of Biology. 2020; 4(1): 62-66.
IEEE S. Çam and Ç. Küçük , "The Effect of Salinity on Growth, Antagonistic Potential, Protease Activity, and Proline Content of Trichoderma harzianum", Commagene Journal of Biology, vol. 4, no. 1, pp. 62-66, Jun. 2020, doi:10.31594/commagene.738313