Year 2020, Volume 4 , Issue 1, Pages 67 - 73 2020-06-29

Inulinase production capability of a promising medicinal plant: Inula viscosa
Umut verici bir tıbbi bitkinin inulinaz üretim kapasitesi: Inula viscosa

Özden CANLI TAŞAR [1]


The present study was designed to examine the inulinase production capability of Rhodotorula glutinis SO-28 by using Inula viscosa, a promising medicinal plant, as sole carbon source in submerged fermentation. Inula viscosa, a perennial member of Asteraceae family, is a popular and widespread medicinal plant in the Mediterranean region. It is termed as “yapışkan andız otu” in Turkey and has been widely used in folk medicine since the ancient times. Taguchi design of experiment (DOE) technique was utilized for the inulinase production optimization process. An orthogonal array layout of L16 was utilized with four influential factors as following: Inula viscosa amount, agitation speed, incubation temperature, and incubation time at four levels. The obtained results showed that optimized inulinase production enhanced enzyme activity as 99.63 U/ml which was 5-fold higher than the unoptimized condition. In brief, Inula viscosa can be used effectively for inulinase production and use of statistical optimization techniques like Taguchi DOE significantly increases the enzyme yield.

Bu çalışma, batık kültür fermantasyonunda tek karbon kaynağı olarak umut verici bir tıbbi bitki olan Inula viscosa kullanılarak Rhodotorula glutinis SO-28'in inulinaz üretim kapasitesini incelemek üzere tasarlanmıştır. Asteraceae familyasının çok yıllık bir üyesi olan Inula viscosa, Akdeniz bölgesinde popüler ve yaygın bir tıbbi bitkidir. Türkiye'de “yapışkan andız otu” olarak adlandırılmakta ve eski çağlardan beri halk tıbbında yaygın olarak kullanılmaktadır. İnülinaz üretim optimizasyonu sürecinde Taguchi deney tasarımı (DOE) tekniği kullanılmıştır. Inula viscosa miktarı, çalkalama hızı, inkübasyon sıcaklığı ve inkübasyon süresi olmak üzere dört etkin faktörlü ve dört seviyeli olan L16 ortogonal dizilim kullanılmıştır. Elde edilen sonuçlar, optimize edilmiş inulinaz üretiminin enzim aktivitesini, optimize edilmemiş durumdan 5 kat daha yüksek olan 99.63 U / ml olarak arttırdığını göstermiştir. Kısacası Inula viscosa, inülinaz üretiminde etkin bir şekilde kullanılabilir ve Taguchi DOE gibi istatistiksel optimizasyon tekniklerinin kullanımı enzim verimini azımsanmayacak ölçüde artırmaktadır.

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Primary Language en
Subjects Biology
Journal Section Research Articles
Authors

Orcid: 0000-0002-4313-5373
Author: Özden CANLI TAŞAR (Primary Author)
Institution: ERZURUM TEKNİK ÜNİVERSİTESİ
Country: Turkey


Thanks All the experiments were done in the laboratories of High Technology Application and Research Centre (YUTAM), Erzurum Technical University, Erzurum, Turkey.
Dates

Application Date : June 3, 2020
Acceptance Date : June 23, 2020
Publication Date : June 29, 2020

Bibtex @research article { commagene747618, journal = {Commagene Journal of Biology}, issn = {}, eissn = {2602-456X}, address = {Adıyaman Bilimsel Araştırmalar Derneği,}, publisher = {ABADER (Adıyaman Bilimsel Araştırmalar Derneği)}, year = {2020}, volume = {4}, pages = {67 - 73}, doi = {10.31594/commagene.747618}, title = {Inulinase production capability of a promising medicinal plant: Inula viscosa}, key = {cite}, author = {Canlı Taşar, Özden} }
APA Canlı Taşar, Ö . (2020). Inulinase production capability of a promising medicinal plant: Inula viscosa . Commagene Journal of Biology , 4 (1) , 67-73 . DOI: 10.31594/commagene.747618
MLA Canlı Taşar, Ö . "Inulinase production capability of a promising medicinal plant: Inula viscosa" . Commagene Journal of Biology 4 (2020 ): 67-73 <https://dergipark.org.tr/en/pub/commagene/issue/52124/747618>
Chicago Canlı Taşar, Ö . "Inulinase production capability of a promising medicinal plant: Inula viscosa". Commagene Journal of Biology 4 (2020 ): 67-73
RIS TY - JOUR T1 - Inulinase production capability of a promising medicinal plant: Inula viscosa AU - Özden Canlı Taşar Y1 - 2020 PY - 2020 N1 - doi: 10.31594/commagene.747618 DO - 10.31594/commagene.747618 T2 - Commagene Journal of Biology JF - Journal JO - JOR SP - 67 EP - 73 VL - 4 IS - 1 SN - -2602-456X M3 - doi: 10.31594/commagene.747618 UR - https://doi.org/10.31594/commagene.747618 Y2 - 2020 ER -
EndNote %0 Kommagene Biyoloji Dergisi Inulinase production capability of a promising medicinal plant: Inula viscosa %A Özden Canlı Taşar %T Inulinase production capability of a promising medicinal plant: Inula viscosa %D 2020 %J Commagene Journal of Biology %P -2602-456X %V 4 %N 1 %R doi: 10.31594/commagene.747618 %U 10.31594/commagene.747618
ISNAD Canlı Taşar, Özden . "Inulinase production capability of a promising medicinal plant: Inula viscosa". Commagene Journal of Biology 4 / 1 (June 2020): 67-73 . https://doi.org/10.31594/commagene.747618
AMA Canlı Taşar Ö . Inulinase production capability of a promising medicinal plant: Inula viscosa. Comm. J. Biol.. 2020; 4(1): 67-73.
Vancouver Canlı Taşar Ö . Inulinase production capability of a promising medicinal plant: Inula viscosa. Commagene Journal of Biology. 2020; 4(1): 67-73.
IEEE Ö. Canlı Taşar , "Inulinase production capability of a promising medicinal plant: Inula viscosa", Commagene Journal of Biology, vol. 4, no. 1, pp. 67-73, Jun. 2020, doi:10.31594/commagene.747618