Çiğ Süt ve Tereyağı Örneklerinde Aflatoksin M1 Aranması

Year 2024, Volume: 8 Issue: 2, 87 - 96

Mohammed Nooruldeen Saeed Hizo , Hasan Solmaz , Miraç Uçkun

https://doi.org/10.31594/commagene.1550152

Abstract

Bu çalışmada, 2022 ilkbahar mevsiminde, Türkiye ve Irak’taki bazı yerleşim yerlerinde tüketime sunulan inek, koyun, keçi, manda çiğ sütlerinde ve inek tereyağında aflatoksin M1 (AFM1) varlığı araştırıldı ve yasal mevzuatlar dikkate alınarak insan sağlığı yönünden tehlike oluşturup oluşturmadığı belirlendi. Sütlerdeki toksin içerikleri, bu örnekleme noktaları arasında karşılaştırıldı. Çalışmada, 50 adet çiğ inek sütü, 60 adet çiğ koyun sütü, 30 adet çiğ keçi sütü, 30 adet çiğ manda sütü ve 40 adet tereyağı örneği toplanarak AFM1 varlığı ELISA yöntemi ile analiz edildi. Ankara’dan toplanan koyu ve keçi sütü örneklerindeki AFM1 düzeyinin, Musul’dan toplananlara göre istatistiksel olarak önemli düzeyde yüksek olduğu, ancak Avrupa Birliği ve Türk Gıda Kodeksi (TGK) limitini (50 ng/kg) aşmadığı görüldü. Musul’dan örneklenen manda sütü ve inek tereyağındaki AFM1 düzeylerinin, sırasıyla Kayseri ve Ankara’da örneklenenlere göre istatistiksel olarak önemli düzeyde yüksek olduğu ve TGK limitlerini aştığı gözlendi. İnek sütü örneklerinden ise Ankara ve Musul’dan örneklenenlerin AFM1 düzeyinin TGK limitini aştığı tespit edildi. Sonuç olarak, Musul’dan toplanan manda sütü, inek sütü ve inek tereyağı ile Ankara’dan toplanan inek sütü örneklerinde tespit edilen AFM1 varlığının halk sağlığı açısından önemli olması nedeniyle, bu bölgelerde, yem olarak kullanılan tarımsal ürünlerin doğru seçimi, uygun koşullarda muhafaza edilmesi, rutin olarak aflatoksin yönünden analiz edilmesi ve sıkı bir şekilde denetlenmesi gerekmektedir. Ayrıca, besi yetiştiriciliği yapan kişiler ile süt ve süt ürünleri üreticilerinin bilgilendirilmesine yönelik iyi tarım uygulamaları konusunda eğitim programları düzenlenerek üretici ve tüketici bilincinin arttırılması sonucu halk sağlığının korunacağı düşünülmektedir.

Keywords

Mikotoksin, Tereyağı, ELISA, Süt

Ethical Statement

Bu çalışma için etik kurul onayı alınmasına gerek yoktur.

Supporting Institution

Karabük Üniversitesi, Bilimsel Araştırma Projeleri Birimi

Project Number

KBÜBAP-22YL-021

Thanks

Bu çalışma Karabük Üniversitesi, Bilimsel Araştırma Projeleri birimi tarafından KBÜBAP-22YL-021 numaralı proje ile maddi olarak desteklenmiştir.

Search for Aflatoxin M1 in Raw Milk and Butter Samples

Abstract

In this study, the presence of aflatoxin M1 (AFM1) in raw milk of cows, sheep, goats, buffalo, and cow butter offered for consumption in some settlements in Turkey and Iraq in the Spring of 2022 was investigated and, taking into account legal regulations, it was determined whether it poses a danger to human health. Toxin contents in milk were compared between these sampling points. In the study, 50 raw cow milk, 60 raw sheep milk, 30 raw goat milk, 30 raw buffalo milk, and 40 butter samples were collected and AFM1 presence was analyzed by ELISA method. It was observed that the AFM1 level in the dark and goat milk samples collected from Ankara was statistically significantly higher than those collected from Mosul but did not exceed the European Union and Turkish Food Codex (TFC) limits (50 ng/kg). AFM1 levels in buffalo milk and cow butter sampled from Mosul were observed to be statistically significantly higher than those sampled in Kayseri and Ankara, respectively, and exceeded the TFC limits. It was determined that the AFM1 level of cow milk samples from Ankara and Mosul exceeded the TFC limit. As a result, since the presence of AFM1 detected in buffalo milk, cow milk, and cow butter samples collected from Mosul and cow milk samples collected from Ankara is important for public health, agricultural products used as feed in these regions should be selected correctly, stored under appropriate conditions, routinely analyzed for aflatoxin, and strictly inspected. Furthermore, the organization of training programs on good agricultural practices aimed at educating livestock breeders and milk and dairy product producers and raising producer and consumer awareness are believed to safeguard public health.

Keywords

Mikotoksin, ELISA, Milk, Butter

Ethical Statement

There is no need for ethics committee approval for this study.

Supporting Institution

Karabuk University, Scientific Research Projects Unit

Project Number

KBÜBAP-22YL-021

Thanks

This study was financially supported by Karabük University, Scientific Research Projects Unit with project number KBÜBAP-22YL-021.

References

• Agriopoulou, S., Stamatelopoulou, E., & Varzakas, T. (2020). Advances in occurrence, importance, and mycotoxin control strategies: Prevention and detoxification in foods”, Foods, 9(2), 137. https://doi.org/10.3390/foods9020137
• Aksoy, A., & Sezer, Ç. (2019). Kars İlinde Tüketime Sunulan Çiğ Süt ve Bazı Peynir Çeşitlerinde Aflatoksin M1 Varlığının Değerlendirilmesi. Kocatepe Veterinary Journal, 12(1), 39-44. https://doi.org/10.30607/kvj.479157
• Azam, M., Ahmed, S., Islam, M., Maitra, P., & Yu, D. (2021). Critical assessment of mycotoxins in beverages and their control measures. Toxins, 13(5), 323. https://doi.org/10.3390/toxins13050323
• Bahrami, R., Shahbazi, Y., & Nikousefat, Z. (2016). Aflatoxin M1 in milk and traditional dairy products from west part of Iran: Occurrence and seasonal variation with an emphasis on risk assessment of human exposure. Food Control, 62, 250-256. https://doi.org/10.1016/j.foodcont.2015.10.039
• Bilandžić, N., Varga, I., Varenina, I., Solomun Kolanović, B., Božić Luburić, Đ., Đokić, M., Cvetnić, L., & Cvetnić, Ž. (2022). Seasonal occurrence of aflatoxin M1 in raw milk during a five-year period in Croatia: Dietary exposure and risk assessment. Foods, 11(13), 1959. https://doi.org/10.3390/foods11131959
• Buldu, H., Koc, A.N., & URAZ, G. (2011). Aflatoxin M1 contamination in cow’s milk in Kayseri (central Turkey). Turkish Journal of Veterinary & Animal Sciences, 35(2), 87-91. https://doi.org/10.3906/vet-0810-5
• De Souza, C., Khaneghah, A.M., & Oliveira, C.A.F. (2021). The occurrence of aflatoxin M1 in industrial and traditional fermented milk: A systematic review study. Italian Journal of Food Science, 33, 12-23. https://doi.org/10.15586/ijfs.v33iSP1.1982
• Elzupir, A.O., & Elhussein, A.M. (2010). Determination of aflatoxin M1 in dairy cattle milk in Khartoum State, Sudan. Food control, 21(6), 945-946. https://doi.org/10.1016/j.foodcont.2009.11.013
• Fallah, A.A., Fazlollahi, R., & Emami, A. (2016). Seasonal study of aflatoxin M1 contamination in milk of four dairy species in Yazd, Iran. Food Control, 68, 77-82. https://doi.org/10.1016/j.foodcont.2016.03.018
• Flores-Flores, M.E., & González-Peñas, E. (2017). An LC–MS/MS method for multi-mycotoxin quantification in cow milk. Food Chemistry, 218, 378-385. https://doi.org/10.1016/j.foodchem.2016.09.101
• Ghaffarian Bahraman, A., Mohammadi, S., Jafari, A., Ghani-Dehkordid, J., Arabnezhad, M.R., Rahmdel, S., & Hosseini Teshnizi, S. (2020). Occurrence of aflatoxin M1 in milks of five animal species in Iran: a systematic review and meta-analysis. Food Reviews International, 36(7), 692-712. https://doi.org/10.1080/87559129.2019.1669164
• Ghanem, I., & Orfi, M. (2009). Aflatoxin M1 in raw, pasteurized and powdered milk available in the Syrian market. Food Control, 20(6), 603-605. https://doi.org/10.1016/j.foodcont.2008.08.018
• Gide, S., Muhammed, B., Galal, G.H., Iliyah, A.A., Mabu, I.M., Lawan, M.S., Muhammad, Y., & Sheriff, W. (2020). Determination of aflatoxin M1 in raw milk produce at Yobe State University Farm Damaturu, Nigeria. Fudma Journal of Sciences, 4(3), 457-46. https://doi.org/10.33003/fjs-2020-0403-413
• Hashemi, M., (2016). A survey of aflatoxin M1 in cow milk in Southern Iran. Journal of food and drug analysis, 24(4), 888-893. https://doi.org/10.1016/j.jfda.2016.05.002
• Hussain, I., Anwar, J., Asi, M.R., Munawar, M.A., & Kashif, M. (2010). Aflatoxin M1 contamination in milk from five dairy species in Pakistan. Food control, 21(2), 122-124. https://doi.org/10.1016/j.foodcont.2008.12.004
• Iqbal, S.Z., Asi, M.R., & Ariño, A. (2011). Aflatoxin M1 contamination in cow and buffalo milk samples from the North West Frontier Province (NWFP) and Punjab provinces of Pakistan. Food Additives and Contaminants: Part B, 4(4), 282-288. https://doi.org/10.1080/19393210.2011.637237
• İşleyici, Ö., Morul, F., & Sancak, Y.C. (2012). Van'da tüketime sunulan UHT sterilize inek sütlerinde aflatoksin M1 düzeyinin araştirilması. Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi, 23(2), 65-69.
• İşleyici, Ö., Sancak, Y.C., Sancak, H., & Yücel, U.M. (2015). Determination of Aflatoxin M1 Levels in Unpackaged Sold Raw Cow's Milk. Van Veterinary Journal, 26(3), 151-155.
• Karadal, F., Onmaz, N.E., Hızlısoy, H., Yıldırım, Y., Serhat, A.L., & Gönülalan, Z. (2018). Niğde ilindeki çiğ koyun, keçi ve inek sütlerinde aflatoksin M1 düzeyleri. Kocatepe Veterinary Journal, 11(2), 119-125. https://doi.org/10.30607/kvj.376355
• Karaoğlan, H., Yanık, E.D., & Tunç, N. (2022). Ülkemizde ve dünyada süt ve süt ürünlerinde aflatoksin M1 varlığı. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 53(1), 81-87. https://doi.org/10.54614/AUAF.2022.995228
• Karimi Dehcheshmeh, B., Shakerian, A., & Rahimi, E. (2021). Evaluation of aflatoxin M1 and heavy metal in raw materials and infant formula produced in Pegah dairy plants, Iran. Journal of Chemical Health Risks, 11(1), 55-62. https://doi.org/10.22034/jchr.2020.1907145.1166
• Mahmoudi, R. (2014). Seasonal pattern of aflatoxin M1 contamination in buffalo milk. Journal of Agroalimentary Processes and Technologies, 20(1), 9-13.
• Matabaro, E., Ishimwe, N., Uwimbabazi, E., & Lee, B.H. (2017). Current immunoassay methods for the rapid detection of aflatoxin in milk and dairy products. Comprehensive Reviews in Food Science and Food Safety, 16(5), 808-820. https://doi.org/10.1111/1541-4337.12287
• Mehenktaş, C. (2019). Süt ve süt ürünlerinde aflatoksin M1. Akademik Gıda, 17(3), 439-443. https://doi.org/10.24323/akademik-gida.647735
• Merve, A.Ç.U. & Ocak, Ö.Ö. (2019). Gıdalarda aflatoksin düzeylerinin belirlenmesinde kullanılan analiz yöntemleri. Sinop Üniversitesi Fen Bilimleri Dergisi, 4(2), 168-181. https://doi.org/10.33484/sinopfbd.537820
• Negash, D. (2018). A review of aflatoxin: occurrence, prevention, and gaps in both food and feed safety. Journal of Applied Microbiological Research, 1 (1), 35-43. https://doi.org/10.3619/2581-7566.1000106
• Nile, S.H., Park, S. W., & Khobragade, C.N. (2016). Occurrence and analysis of aflatoxin M1 in milk produced by Indian dairy species. Food and Agricultural Immunology, 27(3), 358-366. https://doi.org/10.1080/09540105.2015.1104655
• Özkan, N.A., & Onmaz, N.E. (2019). Perakende Olarak Satışa Sunulan Kaymak ve Tereyağlarda Aflatoksin M1 Varlığı. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 16(3), 213-217. https://doi.org/10.32707/ercivet.648571
• Ráduly, Z., Szabó, L., Madar, A., Pócsi, I., & Csernoch, L. (2020). Toxicological and medical aspects of Aspergillus-derived mycotoxins entering the feed and food chain. Frontiers in Microbiology, 10, 2908. https://doi.org/10.3389/fmicb.2019.02908
• Rahimi, E., Bonyadian, M., Rafei, M., & Kazemeini, H.R. (2010). Occurrence of aflatoxin M1 in raw milk of five dairy species in Ahvaz, Iran. Food and Chemical Toxicology, 48(1), 129-131. https://doi.org/10.1016/j.fct.2009.09.028
• Roila, R., Branciari, R., Verdini, E., Ranucci, D., Valiani, A., Pelliccia, A., Fioroni, L., & Pecorelli, I. (2021). A Study of the Occurrence of Aflatoxin M1 in Milk Supply Chain over a Seven-Year Period (2014–2020): Human Exposure Assessment and Risk Characterization in the Population of Central Italy, Foods. 10(7), 1529. https://doi.org/10.3390/foods10071529
• Thukral, H., Dhaka, P., Bedi, J.S., & Aulakh, R.S. (2022). Occurrence of aflatoxin M1 in bovine milk and associated risk factors among dairy farms of Punjab, India. World Mycotoxin Journal, 15(2), 201-210. https://doi.org/10.3920/WMJ2021.2697
• Tomašević, I., Petrović, J., Jovetić, M., Raičević, S., Milojević, M., & Miočinović, J. (2015). Two year survey on the occurrence and seasonal variation of aflatoxin M1 in milk and milk products in Serbia. Food control, 56, 64-70. https://doi.org/10.1016/j.foodcont.2015.03.017
• Türkoğlu, Ç. (2018). Çiğ, pastörize ve UHT sütlerde Aflatoksin M1 ve Okratoksin A varlığının araştırılması, Mehmet Akif Ersoy Üniversitesi, Sağlık Bilimleri Enstitüsü, Hayvansal Ürünler Hijyen ve Teknolojisi Anabilim Dalı, Yüksek Lisans Tezi, Burdur, sy 3-18.
• Yurt, B., & Uluçay, B. (2017). Determination of some chemical properties and aflatoksin M1 of milk produced in Iğdır. Turkish Journal of Nature and Science, 6(2), 32-39.