Kovalius (Opiliones: Sclerosomatidae: Leiobuninae) Cinsinin İlk Moleküler Verileri ve Filogenetik İlişkileri
Year 2025,
Volume: 9 Issue: 1, 59 - 63, 30.06.2025
Pınar Kurt
,
Nalan Yıldırım Doğan
Abstract
Kovalius (Opiliones: Sclerosomatidae: Leiobuninae) Tchemeris tarafından 2023 yılında tanımlanan küçük bir otbiçen cinsidir. Kovalius logunovi'nin ilk tanımı Rusya'dan yapılmış ve daha sonra morfolojik verilere dayanarak Türkiye'den kaydedilmiştir. Taksonların tanımlanması ve ilişkilerinin belirlenmesi için sadece morfolojik verilerin kullanılması bazı durumlarda sınırlamalara ve zorluklara yol açabilir. Sonuç olarak, yeni taksonların tanımlanmasında ve filogenetik ilişkilerin belirlenmesinde morfolojik verilere ek olarak moleküler verilerin de kullanılması çalışmaların güvenilirliğini artırmaktadır. Bu çalışmada Kovalius logunovi türünün mitokondriyal 16S rRNA gen bölgesi ilk kez çalışılmış ve yaklaşık 408 bp'lik bir dizi elde edilmiştir. Bu verilere dayanarak türün benzer türlerle olan filogenetik ilişkileri ortaya konmuştur.
Ethical Statement
Bu çalışma için etik kurul iznine ihtiyaç yoktur.
Thanks
Bu çalışma için örneklerinin toplanması ve tanımlanmasından dolayı Prof. Dr. Kemal KURT'a (Gümüşhane Üniversitesi, Türkiye) teşekkür ederiz.
References
-
Akaike, H. (1998) Information theory and an extension of the maximum likelihood principle. In: Parzen, E., Tanabe, K. &Kitagawa, G. (Eds.), Selected Papers of Hirotugu Akaike. Springer, New York, New York, pp. 199–213.
-
Altschul, S.F., Gish, W., Miller, W., Myers, E.W., & Lipman, D.J. (1990). Basic local alignment search tool. Journal of molecular biology, 215(3), 403-410.
-
Alyamani, N.M. (2024). Mitochondrial 16S rRNA gene as a molecular marker in the phylogenetic relationships of some Rabbitfishes species (Siganidae: Perciformes). Open Veterinary Journal, 14(8), 1936.
-
Burns, M., Hedin, M., & Shultz, J.W. (2012). Molecular phylogeny of the leiobunine harvestmen of eastern North America (Opiliones: Sclerosomatidae: Leiobuninae). Molecular Phylogenetics and Evolution, 63(2), 291-298.
-
Chan, K.O., Hertwig, S.T., Neokleous, D.N., Flury, J.M., & Brown, R.M. (2022). Widely used, short 16S rRNA mitochondrial gene fragments yield poor and erratic results in phylogenetic estimation and species delimitation of amphibians. BMC Ecology and Evolution, 22(1), 37.
-
Doğan, N.Y., & Kurt, P. (2019). DNA barcoding and phylogenetic analysis of Nelima pontica Charitonov, 1941 (Opiliones: Sclerosomatidae) based on mitochondrial COI and 16S rRNA genes. Acta Biologica Turcica, 33(1), 8-11.
-
Dress, A.W., Flamm, C., Fritzsch, G., Grünewald, S., Kruspe, M., Prohaska, S.J., & Stadler, P.F. (2008). Noisy: identification of problematic columns in multiple sequence alignments. Algorithms for Molecular Biology, 3, 1-10.
-
Edgecombe, G.D., Wilson, G.D.F., Colgan, D.J., Gray, M.R., & Cassis, G. (2000). Arthropodcladistics: combined analysis of histone H3 and U2 snRNA sequences andmorphology. Cladistics, 16, 155-203.
-
Hedin, M., Tsurusaki, N., Macías-Ordóñez, R., & Shultz, J.W. (2012). Molecular systematics of sclerosomatid harvestmen (Opiliones, Phalangioidea, Sclerosomatidae): geography is better than taxonomy in predicting phylogeny. Molecular Phylogenetics and Evolution, 62(1), 224-236.
-
Huelsenbeck, J.P., & Ronquist, F. (2001). MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics, 17(8), 754-755.
-
Kaleshkumar, K., Rajaram, R., Vinothkumar, S., Ramalingam, V., & Meetei, K.B. (2015). Note DNA barcoding of selected species of pufferfishes (Order: Tetraodontiformes) of Puducherry coastal waters along south-east coast of India. Indian Journal of Fisheries, 62(2), 98–103.
-
Kumar, S., Stecher, G., Suleski, M., Sanderford, M., Sharma, S., & Tamura, K. (2024). MEGA12: Molecular Evolutionary Genetic Analysis version 12 for adaptive and green computing. Molecular Biology and Evolution, 41(12), msae263.
-
Kurt, K. (2014). Updated checklist of harvestmen (Arachnida: Opiliones) in Turkey. Archives of Biological Sciences, 66(4), 1617-1631.
-
Kurt, K. (2024). A New Harvestman Genus Record for Turkey: Kovalius Tchemeris, 2023 (Opiliones Sclerosomatidae, Leiobuninae). Entomological News, 131(4), 186-192.
-
Lemoine, F., Correia, D., Lefort, V., Doppelt-Azeroual, O., Mareuil, F., Cohen-Boulakia, S., & Gascuel, O. (2019). NGPhylogeny. fr: new generation phylogenetic services for non-specialists. Nucleic acids research, 47(W1), W260-W265.
-
Letunic, I., & Bork, P. (2021). Interactive Tree of Life (iTOL) v5: an online tool for phylogenetic tree display and annotation. Nucleic Acids Research, 49(W1), W293–W296.
-
Nei, M., & Kumar, S. (2000). Molecular evolution and phylogenetics. Oxford university press.
-
Saitou, N., & Nei, M. (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular biology and evolution, 4(4), 406-425.
-
Sievers, F., Wilm, A., Dineen, D., Gibson, T. J., Karplus, K., Li, W., ..., & Higgins, D. G. (2011). Fast, scalable generation of high‐quality protein multiple sequence alignments using Clustal Omega. Molecular systems biology, 7(1), 539.
-
Tchemeris, A.N. (2023). Kovalius - a new genus of cave-dwelling harvestmen from the Caucasus (Opiliones: Sclerosomatidae: Leiobuninae). Zootaxa, 5227(4), 486-494.
-
Wang, Z.L., Yang, X.Q., Wang, T.Z., & Yu, X. (2018). Assessing the effectiveness of mitochondrial COI and 16S rRNA genes for DNA barcoding of farmland spiders in China. Mitochondrial DNA Part A, 29(5), 695-702.
-
Xiong, B., & Kocher, T. D. (1991). Comparison of mitochondrial DNA sequences of seven morphospecies of black flies (Diptera: Simuliidae). Genome, 34(2), 306-311.
First Molecular Data for the Genus Kovalius (Opiliones: Sclerosomatidae: Leiobuninae) and their Phylogenetic Relationships
Year 2025,
Volume: 9 Issue: 1, 59 - 63, 30.06.2025
Pınar Kurt
,
Nalan Yıldırım Doğan
Abstract
Kovalius (Opiliones: Sclerosomatidae: Leiobuninae) is a small genus of harvestmen described by Tchemeris, 2023. The first description of Kovalius logunovi was made from Russia and it was subsequently recorded from Türkiye on the basis of the morphological data. The use of morphological data alone for the identification of taxa and determination of their relationships may lead to limitations and difficulties in some cases. As a result, the use of molecular data in addition to the morphological data in the identification of new taxa and determination of phylogenetic relationships increases the reliability of studies. In this study, the mitochondrial 16S rRNA gene region of the Kovalius logunovi species was examined for the first time and a sequence of approximately 408 bp was obtained. Based on these data, the phylogenetic relationships of the species with similar species were revealed.
Ethical Statement
Ethics committee approval is not required for this study.
Thanks
We are grateful to Prof. Dr Kemal KURT (Gümüşhane University, Turkey) for the collection and identification of harvester specimens for this study.
References
-
Akaike, H. (1998) Information theory and an extension of the maximum likelihood principle. In: Parzen, E., Tanabe, K. &Kitagawa, G. (Eds.), Selected Papers of Hirotugu Akaike. Springer, New York, New York, pp. 199–213.
-
Altschul, S.F., Gish, W., Miller, W., Myers, E.W., & Lipman, D.J. (1990). Basic local alignment search tool. Journal of molecular biology, 215(3), 403-410.
-
Alyamani, N.M. (2024). Mitochondrial 16S rRNA gene as a molecular marker in the phylogenetic relationships of some Rabbitfishes species (Siganidae: Perciformes). Open Veterinary Journal, 14(8), 1936.
-
Burns, M., Hedin, M., & Shultz, J.W. (2012). Molecular phylogeny of the leiobunine harvestmen of eastern North America (Opiliones: Sclerosomatidae: Leiobuninae). Molecular Phylogenetics and Evolution, 63(2), 291-298.
-
Chan, K.O., Hertwig, S.T., Neokleous, D.N., Flury, J.M., & Brown, R.M. (2022). Widely used, short 16S rRNA mitochondrial gene fragments yield poor and erratic results in phylogenetic estimation and species delimitation of amphibians. BMC Ecology and Evolution, 22(1), 37.
-
Doğan, N.Y., & Kurt, P. (2019). DNA barcoding and phylogenetic analysis of Nelima pontica Charitonov, 1941 (Opiliones: Sclerosomatidae) based on mitochondrial COI and 16S rRNA genes. Acta Biologica Turcica, 33(1), 8-11.
-
Dress, A.W., Flamm, C., Fritzsch, G., Grünewald, S., Kruspe, M., Prohaska, S.J., & Stadler, P.F. (2008). Noisy: identification of problematic columns in multiple sequence alignments. Algorithms for Molecular Biology, 3, 1-10.
-
Edgecombe, G.D., Wilson, G.D.F., Colgan, D.J., Gray, M.R., & Cassis, G. (2000). Arthropodcladistics: combined analysis of histone H3 and U2 snRNA sequences andmorphology. Cladistics, 16, 155-203.
-
Hedin, M., Tsurusaki, N., Macías-Ordóñez, R., & Shultz, J.W. (2012). Molecular systematics of sclerosomatid harvestmen (Opiliones, Phalangioidea, Sclerosomatidae): geography is better than taxonomy in predicting phylogeny. Molecular Phylogenetics and Evolution, 62(1), 224-236.
-
Huelsenbeck, J.P., & Ronquist, F. (2001). MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics, 17(8), 754-755.
-
Kaleshkumar, K., Rajaram, R., Vinothkumar, S., Ramalingam, V., & Meetei, K.B. (2015). Note DNA barcoding of selected species of pufferfishes (Order: Tetraodontiformes) of Puducherry coastal waters along south-east coast of India. Indian Journal of Fisheries, 62(2), 98–103.
-
Kumar, S., Stecher, G., Suleski, M., Sanderford, M., Sharma, S., & Tamura, K. (2024). MEGA12: Molecular Evolutionary Genetic Analysis version 12 for adaptive and green computing. Molecular Biology and Evolution, 41(12), msae263.
-
Kurt, K. (2014). Updated checklist of harvestmen (Arachnida: Opiliones) in Turkey. Archives of Biological Sciences, 66(4), 1617-1631.
-
Kurt, K. (2024). A New Harvestman Genus Record for Turkey: Kovalius Tchemeris, 2023 (Opiliones Sclerosomatidae, Leiobuninae). Entomological News, 131(4), 186-192.
-
Lemoine, F., Correia, D., Lefort, V., Doppelt-Azeroual, O., Mareuil, F., Cohen-Boulakia, S., & Gascuel, O. (2019). NGPhylogeny. fr: new generation phylogenetic services for non-specialists. Nucleic acids research, 47(W1), W260-W265.
-
Letunic, I., & Bork, P. (2021). Interactive Tree of Life (iTOL) v5: an online tool for phylogenetic tree display and annotation. Nucleic Acids Research, 49(W1), W293–W296.
-
Nei, M., & Kumar, S. (2000). Molecular evolution and phylogenetics. Oxford university press.
-
Saitou, N., & Nei, M. (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular biology and evolution, 4(4), 406-425.
-
Sievers, F., Wilm, A., Dineen, D., Gibson, T. J., Karplus, K., Li, W., ..., & Higgins, D. G. (2011). Fast, scalable generation of high‐quality protein multiple sequence alignments using Clustal Omega. Molecular systems biology, 7(1), 539.
-
Tchemeris, A.N. (2023). Kovalius - a new genus of cave-dwelling harvestmen from the Caucasus (Opiliones: Sclerosomatidae: Leiobuninae). Zootaxa, 5227(4), 486-494.
-
Wang, Z.L., Yang, X.Q., Wang, T.Z., & Yu, X. (2018). Assessing the effectiveness of mitochondrial COI and 16S rRNA genes for DNA barcoding of farmland spiders in China. Mitochondrial DNA Part A, 29(5), 695-702.
-
Xiong, B., & Kocher, T. D. (1991). Comparison of mitochondrial DNA sequences of seven morphospecies of black flies (Diptera: Simuliidae). Genome, 34(2), 306-311.