Assessment of Genetic Stability of Propagated Plants of Alyssum caricum Using Flow Cytometry

Abstract

This study aimed to investigate the nuclear DNA content of A. caricum using in vitro-grown plants and to analyse the genetic stability of in vitro-propagated plantlets through flow cytometry. The process involved germinating seeds on Murashige and Skoog medium with 3% sucrose and 0.7% phytoagar. The germinated seedlings were then cultured in vitro in a controlled environment. For the in vitro propagation experiment, nodal explants were cultured on three shoot induction media for 3-4 weeks. Subsequently, the adventitious shoots were transferred to the MS medium with the same formulations as the shoot induction medium to facilitate shoot propagation. The propagated adventitious shoots were then moved to full-strength MS medium with 3% sucrose and 0.7% phytoagar, without any plant growth regulators, to induce root development. The nuclear DNA content was estimated using flow cytometry for both the in vitro germinated seedlings and the propagated plantlets. The analyses revealed that A. caricum had 1.75 pg/2C±0.01 mean nuclear DNA content. Furthermore, the propagated plants exhibited similar nuclear DNA content. These results suggest that the analysed individuals were genetically stable. However, to obtain more comprehensive information about A. caricum, additional studies such as chromosome counting, genetic analyses are necessary.

Keywords

• A. caricum
• In vitro propagation
• Nuclear DNA Content
• Genetic stability
• Flow cytometry

Project Number

FBA-2018-1439

Assessment of Genetic Stability of Propagated Plants of Alyssum caricum Using Flow Cytometry

Abstract

Bu çalışmada, flow sitometri analizi ile in vitro yetiştirilmiş A. caricum bitkilerinin çekirdek DNA içeriğinin belirlenmesi, ayrıca in vitro çoğaltılan bitkilerin genetik stabilitelerinin değerlendirilmesi amaçlanmıştır. Bitkinin tohumları, 3% sukroz ve 0.7% fitoagar içeren Murashige ve Skoog besin ortamında çimlendirilmiştir. Çimlenen fideler kontrollü bir ortamda in vitro olarak büyütülmüştür. In vitro çoğaltım için nodal eksplantlar üç farklı sürgün indüksiyon ortamında 3-4 hafta kültüre alınmıştır. Adventif sürgünler sürgün indüksiyon ortamı ile aynı formülasyonlara sahip MS besin ortamına çoğaltılmaları için aktarılmıştır. Çoğaltılan adventif sürgünler, daha sonra bitki büyüme düzenleyicileri içermeyen MS ortamına aktarılmıştır. In vitro yetiştirilen bitkiler ve in vitro çoğaltılan bitkiler flow sitometri ile analiz edilmiştir. Yapılan flow sitometri analizleri, in vitro yetiştirilen bitkilerin 1.75 pg/2C±0.01 DNA içeriğine sahip olduğunu göstermiştir. Sonuçlar, çoğaltılan bitkilerin tohumdan çimlenen bitkiler ile benzer DNA içeriğine sahip olduğunu göstermiştir. Bu sonuçlar, analiz edilen bireylerin genetik olarak stabil olduğunu önermektedir. Bununla birlikte, A. caricum hakkında daha kapsamlı bilgilere ulaşmak için ek çalışmalar gerekmektedir.

Keywords

• A. caricum
• In vitro çoğaltım
• Çekirdek DNA içeriği
• Genetik stabilite
• Flow sitometri.

Supporting Institution

Çanakkale Onsekiz Mart University The Scientific Research Coordination Unit

Project Number

FBA-2018-1439

Ethical Statement

There are no ethical issues after the publication of this manuscript.

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