Pırlak Koyunlarında Kalpastatin (CAST) ve Miyostatin (MSTN) Gen Polimorfizmleri

Abstract

Bu çalışmada, Akdeniz Bölgesi'nde yaygın olarak yetiştirilen yerli Pırlak koyunlarında, Kalpastatin (CAST) ve Miyostatin (MSTN) genlerinin polimorfik yapısı incelemiştir. Antalya ilindeki farklı işletmelerden 181 baş koyunda PCR-RFLP yöntemi uygulanarak CAST geninde MspI, MSTN geninde ise HaeIII polimorfizmi araştırılmıştır. CAST geninde MM (0.220), MN (0.696) ve NN (0.082) genotipleri tespit edilmiştir. M ve N alel frekansları sırasıyla 0.569 ve 0.431 olarak hesaplanmıştır. Buna karşılık, MSTN gen bölgesi tamamen monomorfik yapı sergileyerek yalnızca mm genotipi bulunmuştur. Ayrıca, CAST geninde Hardy-Weinberg dengesinden sapma olduğu belirlenmiştir. Elde edilen veriler, CAST genindeki polimorfik yapının genetik marker olarak kullanılabileceğini ve moleküler düzeyde yapılacak seleksiyon çalışmalarına katkı sağlayabileceğini ortaya koymuştur.

Keywords

• Koyun
• Kalpastatin
• Miyostatin
• PCR-RFLP
• Polimorfizm

Project Number

FYL-2023-6279

Pırlak Koyunlarında Kalpastatin (CAST) ve Miyostatin (MSTN) Genlerinde Polimorfizm

Abstract

Bu çalışmada, Akdeniz Bölgesi'nde yaygın olarak yetiştirilen yerli Pırlak koyunlarında, Kalpastatin (CAST) ve Miyostatin (MSTN) genlerinin polimorfik yapısı incelenmiştir. Antalya ilindeki farklı işletmelerden 181 baş koyunda PCR-RFLP yöntemi uygulanarak CAST geninde MspI, MSTN geninde ise HaeIII polimorfizmi araştırılmıştır. CAST geninde MM (0.220), MN (0.696) ve NN (0.082) genotipleri tespit edilmiştir. M ve N alel frekansları sırasıyla 0.569 ve 0.431 olarak hesaplanmıştır. Buna karşılık, MSTN gen bölgesi tamamen monomorfik yapı sergileyerek yalnızca mm genotipi bulunmuştur. Ayrıca, CAST geninde Hardy-Weinberg dengesinden sapma olduğu belirlenmiştir. Elde edilen veriler, CAST genindeki polimorfik yapının genetik marker olarak kullanılabileceğini ve moleküler düzeyde yapılacak seleksiyon çalışmalarına katkı sağlayabileceğini ortaya koymuştur.

Keywords

• Koyun
• Kalpastatin
• Miyostatin
• PCR-RFLP
• Polimorfizm

Supporting Institution

Akdeniz Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

FYL-2023-6279

Ethical Statement

Akdeniz Üniversitesi Deney Hayvanları Uygulama ve Araştırma Merkezi

Thanks

Akdeniz Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimine tez çalışmamın uygulanabilmesi için sağladıkları finansal destekten dolayı teşekkür ederim.

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