Epibrassinolid, Gibberellik Asit ve Naftalen Asetik Asittin Bazı Nar Çeşitlerinde Çiçek Tozu Çimlenme Oranlarına Etkisi

Abstract

Bu araştırma, Çanakkale Onsekiz Mart Üniversitesi Dardanos Yerleşkesi içerisindeki nar koleksiyon bahçesinde bulunan ‘Caner I’, ‘Caner II’ çeşitleri ve ‘26–3’ nar tipinde yürütülmüştür. Bitki büyüme düzenleyicilerden epibrassinolid (Epi–B1) 0.001, 0.01, 0.1 ppm, gibberellik asit (GA3) 25, 50, 100 ppm ve naftalen asetik asit (NAA) 0,5, 1,0 ve 2,5 ppm konsantrasyonlarında çiçek tozlarını çimlendirme ortamına uygulanmıştır. Çiçek tozu çimlenme denemeleri in vitro koşullarda ‘agar–petri’ yöntemi ile yapılmıştır. ‘Caner I’, ‘Caner II’ çeşitleri ve ‘26–3’ tipinde bulunan erkek (steril) ve hermafrodit (fertil) çiçek tiplerinin oranları saptanmıştır. Uygulanan büyümeyi düzenleyicilerin çiçek tozlarının çimlenme oranlarına etkisi her iki çiçek tipinde de belirlenmiştir. İncelenen nar tip ve çeşitlerinde yaklaşık olarak %75 steril çiçek ve %25 oranında fertil çiçek belirlenmiştir. En yüksek çiçek tozu çimlenme oranları epibrassinolid uygulamasının 0,1 ppm konsantrasyonunda elde edilmiştir

Keywords

• Punica granatum, Epibrassinolid, Gibberellik asit, NAA, Polen çimlenme

Effect of Epibrassinolide, Gibberellic acid and Naphthalene Acetic Acid on Pollen Germination of Some Pomegranate Cultivars

Abstract

This research was carried out on ‘Caner I’, ‘Caner II’ cultivars and type ‘26–3’ grown at the pomegranate collection orchard located at Dardanos campus of Çanakkale Onsekiz Mart University, Çanakkale, Turkey. Plant growth regulators, epibrassinolide (Epi–B1) at 0.001, 0.01 and 0.1 ppm, gibberellic acid (GA3) at 25, 50 and 100 ppm and naphthalene acetic acid (NAA) at 0.5, 1.0 and 2.5 ppm concentrations were applied to the pollen germination medium. Pollen germination experiments were done using agar in the petri method. Ratios of male (sterile) and hermaphrodite (fertile) flower types were determined on ‘Caner I’, ‘Caner II’ cultivars and type ‘26–3’. Effects of the plant growth regulators on pollen germination ratios were assessed on both types of flowers. Approximately, 25%hermaphrodite and 75%male flowers were found on the cultivars and the type. The highest pollen germination percentages were obtained from the 0.1 ppm Epi–Bl application

Keywords

• Punica granatum, Epibrassinolide, Gibberellic acid, NAA, Pollen germination

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