Şark Tipi Tütün (Nicotiana tabaccum L.) Genotiplerinde Mısır Canavar Otu (Orobanche aegyptiaca Pers.) Bitkisince Saldırıya Uğramış Köklerin Kıyaslanması ve Diallel Analizleri

Abstract

Mısırlı canavar otu (Orobanche aegyptiaca Pers.) bitkisi, parazit bitkiler içerisinde en tehlikeli türlerden olup, tütün (Nicotiana tabaccum L.) gibi birçok bitkiyi olumsuz yönde etkilemektedir. İran‟da, şark tipi tütünlerde bu parazit bitkiye dayanıklı olanlarını bulmak, ıslah çalışmalarında en önemli misyonlardan birisidir.Bu araştırma, bu hedefe ulaşabilmek için Urumiye Tütün Araştırma Merkezi‟nde kurulmuştur. Denemede, diallel analizleri kullanılarak, dayanıklı genotiplerin belirlenmesi hedeflenmiştir. Deneme, 15 tane melez ve 6 tane ebeveyni kıyaslamak amacıyla, iki ayrı araştırmada tesadüf blokları deneme desenine göre 3 tekerrürlü olarak planlanmıştır. Değerlendirilen karakterlerdeki (tütünde yaş ve kuru kök ağırlığı, parazit bitkide yaş ve kuru ağırlık ve bitki sayıları) varyans analizlerine göre, özel kombinasyon yeteneği açısından tütünlerde kuru kök ağırlığı, parazit bitkilerde yaş ve kuru ağırlık ve bitki sayılarındaki farklılıklar anlamlı bulunmuştur. Bu sonuçlar, eklemeli olmayan etki gösteren genlerin önemini ortaya koymaktadır. Genel kombinasyon yeteneği önemli etkiye sahip olmadığından, ebeveynler arasında iyi ve zayıf genel kombinasyon yeteneği bulunamamıştır. Bazı hibritlerde özel kombinasyon yeteneği önemli bulunmuş ve bu nedenle şark tipi tütünlerin ıslah çalışmalarında kullanılabilecekleri tavsiyesi yapılmıştır

Keywords

• Diallel, Gen etkileri, GCA, SCA, Şark Tipi Tütünler, Orobanche aegyptiaca Pers

Invader Shoots with Invaded Roots on Diallel Analysis of Oriental Tobacco Genotypes under Egyptian Broomrape Stress

Abstract

Egyptian broomrape (EBR) (Orobanche aegyptiaca Pers. is one of the most dangerous parasitic plants for a broad spectrum of hosts including tobacco (Nicotiana tabaccum L.). Finding genotypes resistant to EBR in Oriental tobacco, one of the main types of tobacco is of great importance and is one of the breeding aims in fighting against this parasite in Iran. In this strategic direction, a research work using diallel analysis was conducted to identify resistant Oriental tobacco genotypes against EBR at Urmia Tobacco Research Centre, Iran in 2012. Fifteen F1 progenies along with their six parents were planted in randomized complete block design with three replications in two separate experiments. Analysis of variance of investigated traits [fresh weight of root (FWR) and dry weight of root (DWR) for host plants, and fresh weight of broomrape shoot (FWBS), dry weight of broomrape shoot (DWBS) and number of broomrape shoots (NBS) for parasite plants] using diallel analysis showed significant mean squares of specific combining ability (SCA) for DWR, DWBS and NBS indicating the importance of non-additive genetic effects for these traits. Good and poor general combiners were not determined among parents for investigated traits due to non-significance of general combining ability (GCA). A set of hybrids appeared to be significant SCA, which could be used in Oriental tobacco improvement

Keywords

• Diallel, Gene effects, GCA, SCA, Oriental tobacco, Orobanche aegyptiaca Pers

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