Evaluation of the Biocontrol Potential of Morina persica L. Extract against Ditylenchus dipsaci and Some Plant Pathogens

Abstract

In this study antifungal and nematicidal effect of methanol extracts of Morina persica L. was examined. In antifungal activities, plant extract was evaluated against Alternaria solani, Fusarium oxysporum f. sp. lycopersici (FOL) and Verticillium dahliae. The experiment was carried out by the agar plate methods at a concentration of 0.625, 1.25, 2.5, 5, 10 and 20 mg mL. All concentration of M.persica plant extracts was showed a different level of antifungal activities against test fungi depend on concentrations. Nematicidal activity was evaluated against stem nematode Ditylenchus dipsaci (Kühn, 1857) under in vitro condition. Five concentrations (31.25, 62.5, 125, 250 and 500 ppm) of M. persica plant extracts were tested. The nematodes exposed 24, 48, 72 and 96 hours in plant extracts and kept at 25 °C. M. persica plant extracts were found highly effective on D. dipsaci. The plant extracts of Morina persica was first time determined the antifungal and nematicidal activities in this study, and has potential effect as a bio pesticide against plant pathogens and plant parasitic nematodes

Keywords

• Morina persica,Alternaria solani,Fusarium oxysporum f. sp. lycopersici,Verticillium dahliae,Ditylenchus dipsaci

Morina persica L. Ekstraktının Ditylenchus dipsaci ve Bazı Bitki Patojenlerine Karşı Biyolojik Mücadelede Kullanım Potensiyelinin Belirlenmesi

Abstract

Bu çalışmada, Morina persica L. metanol ekstraktının antifungal ve nematisidal etkinliği araştırılmıştır. Antifungal etkinliğini belirlemede bitki ekstraktı Alternaria solani, Fusarium oxysporum f. sp. lycopersici (FOL) ve  Verticillium dahliae patojenlerine karşı denenmiştir. Deneme, M. persica ekstraktının  0.625, 1.25, 2.5, 5, 10 ve 20 mg mL’lik konsantrasyonlarında agar petri yöntemi ile gerçekleştirilmiştir. M. persica ekstraktının tüm konsantrasyonları doza bağlı olarak farklı seviyerlerde antifungal etkinlik göstermiştir.Nematisidal aktivitesi Ditylenchus dipsaci (Kühn, 1857)’ye karşı in vitro koşullarda gerçekleştirilmiştir.  M. persica ekstraktının 5 farklı konsantrasyonu (31.25, 62.5, 125, 250 ve 500 ppm) D.dipsaci ‘ye karşı denenmiştir. Nematodlar,  25 °C’de 24, 48, 72 ve 96 saat bitki ekstraktına maruz bırakılmıştır.  M. persica ekstraktının D.dipsaci’ye karşı oldukça etkili olduğu tespit edilmiştir.  Morina persica L. ekstraktı’nın antifungal ve nematisidal etkinliği ilk kez bu çalışma ile belirlenmiş ve ele alınan nematod ve patojenlere karşı biyopestisit olarak potansiyel bir etkiye sahip olduğu saptanmıştır.   

Keywords

• Morina persica,Alternaria solani,Verticillium dahliae,Ditylenchus dipsaci,Fusarium oxysporum f.sp.lycopersici

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