Gökkuşağı Alabalığı (Oncorhynchus mykiss) Yemlerinde Balık Unu Yerine Kullanılan Acı Bakla Ununun (Lupinus albus) Bazı İmmünolojik Parametreler ve Gen Ekspresyon Seviyeleri Üzerine Etkisi

Abstract

Bu çalışmada, gökkuşağı alabalığı (Oncorhynchus mykiss) yavru yemlerinde balık unu yerine % 0(ABU₀),15(ABU₁₅), 30(ABU₃₀), 45 (ABU₄₅) ve 60 (ABU₆₀) oranlarında acı bakla (Lupinus albus) unu ikamesinin gökkuşağı alabalığı TNF-α, IL-1ß ve IL-8 genlerinin ekspresyon seviyelerindeki değişim ile bazı immünolojik parametreler üzerine etkileri belirlenmiştir. Besleme denemesinde kullanılan yemler temel protein kaynağı balık unu ve acı bakla unu olmak üzere ortalama % 41,5 ham protein ve 18,1 yağ içerecek şekilde hazırlanmıştır. Besleme denemesinde kullanılan yemlerin esansiyel amino asit içeriklerinin tamamı alabalık gereksiniminden yüksek olduğu gözlenmiştir. 60 gün süren deneme sonunda her gruptan 15 balıktan kan ve doku örnekleri alınmıştır. Alınan kan örneklerinden lizozim (LİZ), myeloperoksidaz (MPO) ve respiratöri burst (RBT) aktivitesi analizleri karaciğer doku örneklerinden gökkuşağı alabalığına özgü β-aktin, TNF-α, IL-1ß ve IL-8 genleri üzerinden moleküler tetkikler yapılmıştır. Elde edilen bulgulara göre gökkuşağı alabalığı yavru yemlerinde acı bakla unu kullanımına bağlı olarak TNF-α, IL-1ß ve IL-8 genleri ekspresyon seviyelerinin ve LİZ ve RBA aktivitelerinin artış gösterdiği (p<0,05), MPO aktivitesi üzerine etkisi olmadığı gözlenmiştir. Sonuç olarak acı bakla ununun gökkuşağı alabalığı yemlerinde %60 oranında balık ununa ikame kullanımının gökkuşağı alabalıklarında bağışıklık sistemini olumlu yönde etkilediği neticesine varılmıştır

Keywords

• Oncorhynchus mykiss,Lupinus albus,gen ekspresyonu,bağışıklık sistemi

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