Yara Kaplama Malzemesi olarak Kollajen Esaslı Hidrojel Filmleri

Year 2018, Volume: 4 Issue: 2, 103 - 116, 17.12.2018

Mehtap Sahiner Selin Sagbas Abdullah Turan , Hüseyin Erdugan , Nurettin Sahiner

https://doi.org/10.28979/comufbed.470362

Abstract

Yara kaplama malzemesi olarak kullanılacak kollajen/kitosan (koll/kitosan)
ve kollajen/kitosan/poli(N-izopropil akrilamid) (koll/kitosan/p(NIPAm)) iç içe
geçmiş ağ yapılı (IPN) hidrojelleri gluteraldehit ve poli(etilenglikol)diakrilat
çapraz bağlayıcıları kullanılarak film şeklinde sentezlenmiştir. Hazırlanan
hidrojellerin morfolojik karakterizasyonu optik mikroskop ile yapılmış ve
hidrojel yüzeylerinde 1-20 µm boyutunda mikro gözenekler içerdiği belirlenmiştir.
Hidrojellerin yapısal ve termal karakterizasyonu FT-IR spektroskopisi ve termal
gravimetrik analiz (TGA) ölçümleri ile belirlenmiştir. Koll/kitosan ve
koll/kitosan/p(NIPAm) IPN hidrojellerinin pH 5,4, 7,4 ve 9,0 da denge şişme
kapasiteleri belirlenmiş ve koll/kitosan/p(NIPAm) hidrojeli en yüksek pH 5,4
değerinde % 428±97 oranında şiştiği belirlenmiştir. Ayrıca hidrojellerin 25-50
ºC aralığındaki denge şişme değerleri ölçülmüş ve koll/kitosan hidrojellerinin
yaklaşık % 200 şişme değeri ile neredeyse bütün sıcaklıklarda aynı şişme derecesine
sahip olduğu gözlemlenirken koll/kitosan/p(NIPAm) hidrojelinin 25 ºC sıcaklıkta
% 312±14 oranında şişerken, yapısındaki sıcaklık duyarlı p(NIPAM) den dolayı 50
ºC ısıtıldığında % 59±2 şişme değeri ile küçüldüğü gözlemlenmiştir. Ayrıca, hazırlanan
koll/kitosan esaslı IPN hidrojelleri deksametazon sodyum fosfat ilacının
salımında kullanılmış ve koll/kitosan hidrojellerinin 114,6±2,9 mg/g ilacı 15
saatte salarken koll/kitosan/p(NIPAm) aynı sürede yaklaşık 51,3±1,2 mg/g saldığı
gözlemlenmiştir. 

Keywords

kitosan, NIPAm, hidrojel, yara kaplama, kollajen, ilaç salımı

Collagen based Hydrogel Films as Wound Dressing Materials

Abstract

Collagen/chitosan
(coll/chitosan) and collagen/chitosan/poly(N-isopropylacrylamide)
(coll/chitosan/p(NIPAm)) interpenetrating network (IPN) hydrogels were synthesized
in film form using glutaraldehyde and poly(ethylene glycol)diacrylate as crosslinkers
for the purpose of wound dressing materials. Morphological characterization of
the prepared hydrogels were done by optic microscope images and it was found that
the surface of the hydrogels possess microporous structure with the pore size
of 1-20 µm. The structural and thermal characterizations of the hydrogels were
evaluated by using FT-IR spectroscopy and thermal gravimetric analysis (TGA). The
equilibrium swelling capacity of collagen/chitosan and
collagen/chitosan/p(NIPAm) IPN hydrogels were measured at pH 5.4, 7.4 and 9,0
values and collagen/chitosan/p(NIPAm) hydrogels was found to have the highest equilibrium
swelling capacity was found at pH 5.4 with 428±97 % swelling ratio.  In addition, the equilibrium swelling
properties of the hydrogels between 25 and 50 ºC were measured and observed
that collagen/chitosan hydrogels have the almost same swelling capacity for all
temperatures with nearly 200 % swelling ratio whereas
collagen/chitosan/p(NIPAm) hydrogels have the swelling ratio of 312±14 % at 25
ºC and shrunk to % 59±2 % at 50 ºC due to the thermo responsive p(NIPAm) in the
structure. Moreover, the prepared collagen/chitosan based IPN hydrogels were
used in release of drug and it was observed that collagen/chitosan hydrogels
were released 114.06±2.9 mg/g dexamethasone drug within 15 hours whereas
coll/chitosan/p(NIPAm) were released about 51.3±1.2 mg/g at the same time.

Keywords

chitosan, NIPAm, hydrogel, wound dressing, collagen, drug delivery

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