Plantago major'un Antioksidan Potansiyeli ve Fenolik İçeriği

Abstract

Amaç: Bu çalışma, terapötik potansiyeli sahip, tıbbi bir bitki olan Plantago major'un toprak üstü kısımlarının antioksidan kapasitesini ve biyoaktif bileşik miktarını değerlendirmeyi amaçlamıştır. Yöntem: Toprak üstü kısımlar %70 metanol kullanılarak ekstrakte edilmiş ve çözücü buharlaştırılmıştır. Toplam fenolik içerik (TPC) ve toplam flavonoid içerik (TFC) miktarları belirlenmiştir. Antioksidan kapasitesi DPPH, ABTS ve FRAP analizleri ile değerlendirilmiştir. Bulgular: DPPH serbest radikal süpürme testi IC50 değerini 127,33 ± 1,07 µg/mL olarak gösterirken, ABTS testi IC50 değerini 46,74 ± 2,53 µg/mL olarak ortaya koymuştur. FRAP analizi ise indirgeme gücünün 44,62 ± 0,61 mg TE/g ekstrakt eşdeğerinde olduğunu göstermiştir. Sonuç: Plantago major ekstraktının yüksek fenolik ve flavonoid içeriği, güçlü antioksidan aktivitesine önemli ölçüde katkıda bulunmaktadır. Bu bulgular, bitkinin doğal bir antioksidan kaynağı olarak farmasötik ve nutrasötik endüstrilerinde potansiyel kullanımını desteklemektedir.

Keywords

• ABTS
• Antioksidan
• DPPH
• FRAP
• Plantago major L.

Antioxidant Potential and Phenolic Content of Plantago major

Abstract

Objective: This study aimed to evaluate the antioxidant capacity and bioactive compound content of the aerial parts of Plantago major, a medicinal plant with potential therapeutic applications. Methods: The aerial parts were extracted using 70% methanol, and the solvent was evaporated. Total phenolic content (TPC) and total flavonoid content (TFC) were quantified. Antioxidant capacity was assessed using DPPH, ABTS, and FRAP assays. Results: The DPPH radical scavenging assay showed an IC50 value of 127.33 ± 1.07 µg/mL, while the ABTS assay revealed an IC50 value of 46.74 ± 2.53 µg/mL. The FRAP assay demonstrated a reducing power equivalent to 44.62 ± 0.61 mg TE/g extract. Conclusion: The high phenolic and flavonoid content of Plantago major extract contributes significantly to its strong antioxidant activity. These findings suggest its potential application as a natural antioxidant source in the pharmaceutical and nutraceutical industries.

Keywords

• ABTS
• Antioxidant
• DPPH
• FRAP
• Plantago major L.

References

1. Ahmadi, S. M., Farhoosh, R., Sharif, A., & Rezaie, M. (2020). Structure‐antioxidant activity relationships of luteolin and catechin. Journal of Food Science, 85(2), 298-305. https://doi.org/10.1111/1750-3841.14994
2. Akman, T. Ç., Simsek, S., Kayir, Ö., Aksit, Z., Aksit, H., & Genc, N. (2023). LC‐ESI‐MS/MS Chemical characterization, antioxidant and antidiabetic properties of propolis extracted with organic solvents from eastern anatolia region. Chemistry & Biodiversity, 20(5), e202201189. https://doi.org/10.1002/cbdv.202201189
3. Akyüz, M. (2019). Determination of antioxidant activity of ethanol extract of gölevez [(Colocasia esculenta (L.)] tubers. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 22, 388-394. https://doi.org/10.18016/ksutarimdoga.vi.589216
4. Beara, I. N., Lesjak, M. M., Jovin, E. Đ., Balog, K. J., Anackov, G. T., Orcic, D. Z., & Mimica-Dukic, N. M. (2009). Plantain (Plantago L.) species as novel sources of flavonoid antioxidants. Journal of Agricultural and Food Chemistry, 57(19), 9268-9273. https://doi.org/10.1021/jf902205m
5. Bourne, K. Z., Bourne, N., Reising, S. F., & Stanberry, L. R. (1999). Plant products as topical microbicide candidates: assessment of in vitro and in vivo activity against herpes simplex virus type 2. Antiviral Research, 42(3), 219-226. https://doi.org/10.1016/S0166-3542(99)00020-0
6. Chang, C. C., Yang, M. H., Wen, H. M., & Chern, J. C. (2002). Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis, 10(3), 20-30. https://doi.org/10.38212/2224-6614.2748
7. Daştan, S. D., Daştan, T., Çetinkaya, S., Ateşşahin, D., & Karan, T. (2016). Evaluation of in vitro anticancer effect of Plantago major L. and Plantago lanceolata L. leaf extracts from Sivas. Cumhuriyet Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi, 1(1), 7-14.
8. De Stefano, A., Caporali, S., Di Daniele, N., Rovella, V., Cardillo, C., Schinzari, F., Minieri, M., Pieri, M., Candi, E., & Bernardini, S. (2021). Anti-inflammatory and proliferative properties of luteolin-7-O-glucoside. International Journal of Molecular Sciences, 22(3), 1321. https://doi.org/10.3390/ijms22031321

9. Dorta, D. J., Pigoso, A. A., Mingatto, F. E., Rodrigues, T., Pestana, C. R., Uyemura, S. A., Santos, A. C., & Curti, C. (2008). Antioxidant activity of flavonoids in isolated mitochondria. Phytotherapy Research, 22(9), 1213-1218. https://doi.org/10.1002/ptr.2441
10. Eldesoky, A. H., AbdelRahman, R. F., Ahmed, O. K., Soliman, G. A., Saeedan, A. S., Elzorba, H. Y., Elansary, A. A., & Hattori, M. (2018). Antioxidant and hepatoprotective potential of Plantago major growing in Egypt and its major phenylethanoid glycoside, acteoside. Journal of Food Biochemistry, 42(5), e12567. https://doi.org/10.1111/jfbc.12567 Gözcü, S., Akşit, Z., Şimşek, S., Kandemir, A., Aydın, A., Yılmaz, M., & Akşit, H. (2024). LC-MS/MS characterization and biological activities of Morina persica L.(Caprifoliaceae). Journal of Research in Pharmacy, 28(4), 961-973. 10.29228/jrp.779
11. Gözcü, S., Korkmaz, M., Çorlu, S., & Tuysuz, S. (2024). Traditional uses of medicinal plants in Erzincan province, Türkiye. Journal of Faculty of Pharmacy of Ankara University, 48(1), 34-45. https://doi.org/10.33483/jfpau.1329957 Haddadian, K., Haddadian, K., & Zahmatkash, M. (2014). A review of Plantago plant. Indian Journal of Tradinational Knowledge, 13(4), 681-685.
12. Halliwell, B., Aeschbach, R., Löliger, J., & Aruoma, O. (1995). The characterization of antioxidants. Food and Chemical Toxicology, 33(7), 601-617. https://doi.org/10.1016/0278-6915(95)00024-V
13. Huang, H., Chang, Y. H., Xu, J., Ni, H. Y., Zhao, H., Zhai, B. W., Efferth, T., Gu, C. B., & Fu, Y. J. (2022). Aucubin as a natural potential anti-acute hepatitis candidate: inhibitory potency and hepatoprotective mechanism. Phytomedicine, 102, 154170. https://doi.org/10.1016/j.phymed.2022.154170 Imran, M., Aslam Gondal, T., Atif, M., Shahbaz, M., Batool Qaisarani, T., Hanif Mughal, M., Salehi, B., Martorell, M., & Sharifi‐Rad, J. (2020). Apigenin as an anticancer agent. Phytotherapy Research, 34(8), 1812-1828. https://doi.org/10.1002/ptr.6647
14. Jiang, B., Shen, R., Bi, J., Tian, X., Hinchliffe, T., & Xia, Y. (2015). Catalpol: a potential therapeutic for neurodegenerative diseases. Current Medicinal Chemistry, 22(10), 1278-1291. https://doi.org/10.2174/0929867322666150114151720
15. Kikuzaki, H., Hisamoto, M., Hirose, K., Akiyama, K., & Taniguchi, H. (2002). Antioxidant properties of ferulic acid and its related compounds. Journal of Agricultural and Food Chemistry, 50(7), 2161-2168. https://doi.org/10.1021/jf011348w
16. Kolak, U., Boğa, M., Uruşak, E. A., & Ulubelen, A. (2011). Constituents of Plantago major subsp. intermedia with antioxidant and anticholinesterase capacities. Turkish Journal of Chemistry, 35(4), 637-645. https://doi.org/10.3906/kim-1102-990
17. Li, X., Xie, Y., Li, K., Wu, A., Xie, H., Guo, Q., Xue, P., Maleshibek, Y., Zhao, W., & Guo, J. (2018). Antioxidation and cytoprotection of acteoside and its derivatives: comparison and mechanistic chemistry. Molecules, 23(2), 498. https://doi.org/10.3390/molecules23020498
18. Mazzutti, S., Ferreira, S. R. S., Herrero, M., & Ibañez, E. (2017). Intensified aqueous-based processes to obtain bioactive extracts from Plantago major and Plantago lanceolata. The Journal of Supercritical Fluids, 119, 64-71. https://doi.org/10.1016/j.supflu.2016.09.008
19. McCutcheon, A., Roberts, T., Gibbons, E., Ellis, S., Babiuk, L., Hancock, R., & Towers, G. (1995). Antiviral screening of British Columbian medicinal plants. Journal of Ethnopharmacology, 49(2), 101-110. https://doi.org/10.1016/0378-8741(95)90037-3
20. Núñez Guillén, M. E., da Silva Emim, J. A., Souccar, C., & Lapa, A. J. (1997). Analgesic and anti-inflammatory activities of the aqueous extract of Plantago major L. International Journal of Pharmacognosy, 35(2), 99-104. https://doi.org/10.1076/phbi.35.2.99.13288
21. Ragheb, E. M., Ibrahem, E. S., & Shalaby, R. A. (2021). Potential Protective Effects of Plantago major Extracts against Indomethacin-Induced Gastric Ulcer in Rats. Egyptian Journal of Nutrition and Health, 16(1), 1-20. https://doi.org/10.21608/ejnh.2021.194452
22. Rahamouz-Haghighi, S., Bagheri, K., & Sharafi, A. (2023). Investigating a Simple and Sensitive High-performance Liquid Chromatography Method for Simultaneous Determination of Apigenin, Catalpol, and Gallic Acid Contents in Plantago lanceolata L. and Plantago major L. Pharmaceutical and Biomedical Research, 9(4), 297-310. http://dx.doi.org/10.32598/PBR.9.4.1061.5
23. Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9-10), 1231-1237. https://doi.org/10.1016/s0891-5849(98)00315-3
24. Rice-Evans, C., Miller, N., & Paganga, G. (1997). Antioxidant properties of phenolic compounds. Trends in Plant Science, 2(4), 152-159. http://dx.doi.org/10.1016/S1360-1385(97)01018-2
25. Roca-Garcia, H. (1970). Weeds: a link with past. Boston, The Arnold Arboretum of Harvard University. Samuelsen, A. B. (2000). The traditional uses, chemical constituents and biological activities of Plantago major L. A review. Journal of Ethnopharmacology, 71(1-2), 1-21. https://doi.org/10.1016/s0378-8741(00)00212-9
26. Skari, K. P., Malterud, K. E., & Haugli, T. (1999). Radical scavengers and inhibitors of eazymatic lipid peroxidation from plantago major, a medicinal plant. Special Publication-Royal Society of Chemistry, 240, 200-202.
27. Sousa, D. S. M. d., Ruiz, L. d. S., Pereira, A. F. M., Sani, A. A., Zapata, T. B., Monari, G. P. d. M., Rosa, C. F. d., Fernandes, A., Rall, V. L. M., & Prado, D. G. (2025). Plantago major leaf extract against bacteria and fungi of medical importance. Brazilian Journal of Pharmaceutical Sciences, 61, e24143. http://dx.doi.org/10.1590/s2175-97902025e24143
28. Stanisavljević, I. T., Stojičević, S. S., Veličković, D. T., Lazić, M. L., & Veljković, V. B. (2008). Screening the antioxidant and antimicrobial properties of the extracts from plantain (Plantago major L.) leaves. Separation Science and Technology, 43(14), 3652-3662. https://doi.org/10.1080/01496390802219091
29. Taskova, R., Handjieva, N., Evstatieva, L., & Popov, S. (1999). Iridoid glucosides from Plantago cornuti, Plantago major and Veronica cymbalaria. Phytochemistry, 52(8), 1443-1445. https://doi.org/10.1016/S0031-9422(99)00182-X
30. Tian, C., Liu, X., Chang, Y., Wang, R., Lv, T., Cui, C., & Liu, M. (2021). Investigation of the anti-inflammatory and antioxidant activities of luteolin, kaempferol, apigenin and quercetin. South African Journal of Botany, 137, 257-264. http://dx.doi.org/10.1016/j.sajb.2020.10.022
31. Yiğit, B. M., & Gözcü, S. (2024). Ethnobotanical Study of Medicinal Plants in Ağrı Province, Türkiye: Exploring Traditional Knowledge And Therapeutic Potential. Journal of Faculty of Pharmacy of Ankara University, 48(2), 486-497. https://doi.org/10.33483/jfpau.1402467