Plantago major'un Antioksidan Potansiyeli ve Fenolik İçeriği

Öz

Amaç: Bu çalışma, terapötik potansiyeli sahip, tıbbi bir bitki olan Plantago major'un toprak üstü kısımlarının antioksidan kapasitesini ve biyoaktif bileşik miktarını değerlendirmeyi amaçlamıştır. Yöntem: Toprak üstü kısımlar %70 metanol kullanılarak ekstrakte edilmiş ve çözücü buharlaştırılmıştır. Toplam fenolik içerik (TPC) ve toplam flavonoid içerik (TFC) miktarları belirlenmiştir. Antioksidan kapasitesi DPPH, ABTS ve FRAP analizleri ile değerlendirilmiştir. Bulgular: DPPH serbest radikal süpürme testi IC50 değerini 127,33 ± 1,07 µg/mL olarak gösterirken, ABTS testi IC50 değerini 46,74 ± 2,53 µg/mL olarak ortaya koymuştur. FRAP analizi ise indirgeme gücünün 44,62 ± 0,61 mg TE/g ekstrakt eşdeğerinde olduğunu göstermiştir. Sonuç: Plantago major ekstraktının yüksek fenolik ve flavonoid içeriği, güçlü antioksidan aktivitesine önemli ölçüde katkıda bulunmaktadır. Bu bulgular, bitkinin doğal bir antioksidan kaynağı olarak farmasötik ve nutrasötik endüstrilerinde potansiyel kullanımını desteklemektedir.

Anahtar Kelimeler

• ABTS
• Antioksidan
• DPPH
• FRAP
• Plantago major L.

Antioxidant Potential and Phenolic Content of Plantago major

Abstract

Objective: This study aimed to evaluate the antioxidant capacity and bioactive compound content of the aerial parts of Plantago major, a medicinal plant with potential therapeutic applications. Methods: The aerial parts were extracted using 70% methanol, and the solvent was evaporated. Total phenolic content (TPC) and total flavonoid content (TFC) were quantified. Antioxidant capacity was assessed using DPPH, ABTS, and FRAP assays. Results: The DPPH radical scavenging assay showed an IC50 value of 127.33 ± 1.07 µg/mL, while the ABTS assay revealed an IC50 value of 46.74 ± 2.53 µg/mL. The FRAP assay demonstrated a reducing power equivalent to 44.62 ± 0.61 mg TE/g extract. Conclusion: The high phenolic and flavonoid content of Plantago major extract contributes significantly to its strong antioxidant activity. These findings suggest its potential application as a natural antioxidant source in the pharmaceutical and nutraceutical industries.

Keywords

• ABTS
• Antioxidant
• DPPH
• FRAP
• Plantago major L.

Kaynakça

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