TİYOKOLŞİKOSİD’İN A549 AKCİĞER KANSERİ HÜCRELERİNDEKİ ANTİPROLİFERATİF ETKİLERİNİN ARAŞTIRILMASI

Abstract

Amaç: Bu çalışmanın amacı, Tiyokolşikosid'in A549 insan küçük hücreli dışı akciğer kanseri hücreleri üzerindeki IC₅₀ değerini MTT testi kullanarak belirlemek ve antiproliferatif ve yara iyileştirici etkilerini araştırmaktır. Yöntem: Thiocolchicoside'ın A549 hücreleri üzerindeki sitotoksik ve yara iyileştirici etkileri in vitro olarak değerlendirildi. Hücre canlılığı, IC₅₀ değerlerini hesaplamak için MTT testi ile 24, 48 ve 72 saatlerde değerlendirildi. Ek olarak, hücre göçünü değerlendirmek için in vitro yara iyileşmesi (scratch) deneyi yapılmıştır. Bulgular: Tiyokolşikosidin 24 saatteki IC₅₀ değeri 2,693×10-⁴ M olarak bulunmuştur. MTT test sonuçları yüksek konsantrasyonlarda önemli sitotoksisite gösterirken, daha düşük konsantrasyonların önemli bir etkisi olmamış veya hücre canlılığını artırmıştır. Sitotoksik etkiler zamanla artmış, 48 ve 72. saatlerde daha belirgin hale gelmiştir. Yara iyileşmesi deneylerinde, düşük Tiyokolşikosid konsantrasyonları yara kapanmasını önemli ölçüde arttırırken, yüksek konsantrasyonlar bu etkiyi azaltmıştır. İstatistiksel analiz, 24 saatteki en düşük konsantrasyon hariç, 24 ve 48 saatteki konsantrasyonlar arasında önemli farklılıklar olduğunu ortaya koymuştur. Sonuç: Tiyokolşikosid, A549 hücreleri üzerinde doza ve zamana bağlı sitotoksik etkiler gösterir ve düşük konsantrasyonlarda yara iyileşmesini destekleyebilir, bu da kanser tedavisi ve doku onarımında potansiyel ikili rollere işaret eder.

Keywords

• A549
• Antiproliferatif
• Yara İyileşmesi
• Tiyokolşikosid

Supporting Institution

Bu çalışma TÜBİTAK 2209A lisans katılımlı araştırma projesi tarafından desteklenmektedir.

Project Number

1919B012406535

Ethical Statement

Etik kurul onayına gerek bulunmamaktadır.

Thanks

Bu çalışma TÜBİTAK 2209A lisans katılımlı araştırma projesi tarafından desteklenmektedir. Bu çalışma Seher ATASEVER’in lisans bitirme tezidir.

INVESTIGATION OF THE ANTIPROLIFERATIVE EFFECTS OF THIOCOLCHICOSIDE ON A549 LUNG CANCER CELLS

Abstract

Objective: This study aims to determine the IC₅₀ value of Thiocolchicoside on A549 human non-small cell lung cancer cells using the MTT assay and to investigate its antiproliferative and wound healing effects. Methods: The cytotoxic and wound healing effects of Thiocolchicoside on A549 cells were evaluated in vitro. Cell viability was assessed at 24, 48, and 72 hours by MTT assay to calculate IC₅₀ values. Additionally, an in vitro wound healing (scratch) assay was performed to evaluate cell migration. Results: The IC₅₀ value of Thiocolchicoside at 24 hours was found to be 2.693×10⁻⁴ M. MTT assay results showed significant cytotoxicity at high concentrations, while lower concentrations had no significant effect or increased cell viability. Cytotoxic effects increased over time, being more evident at 48 and 72 hours. In wound healing assays, low concentrations of Thiocolchicoside significantly enhanced wound closure, whereas higher concentrations reduced this effect. Statistical analysis revealed significant differences between concentrations at 24 and 48 hours, except for the lowest concentration at 24 hours. Conclusion: Thiocolchicoside exerts dose- and time-dependent cytotoxic effects on A549 cells and may promote wound healing at low concentrations, suggesting potential dual roles in cancer treatment and tissue repair.

Keywords

• Thiocolchicoside
• A549
• wound healing
• antiproliferative

Supporting Institution

Scientific and Technological Research Council of Turkey (TUBITAK) 2209A

Project Number

1919B012406535

Ethical Statement

Ethics committee approval is not required

Thanks

I would like to thank the Scientific and Technological Research Council of Turkey (TÜBİTAK) for supporting my undergraduate thesis project.

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