Effects of acute topiramate administration on post-traumatic stress disorder in rats

Year 2022, , 301 - 309, 31.03.2022

Mahluga Jafarova Demirkapu , Furkan Cuma Doğan , Sevil Karabag , Ahsen Yilmaz , Aliye Çelikkol , Hasan Raci Yananlı

https://doi.org/10.17826/cumj.1035129

Abstract

Purpose: The aim of this study was to investigate the effects of acute systemic topiramate administration on anxiety index and freezing time, plasma estrogen and progesterone levels, and salivary gland immunoglobulin A in a post-traumatic stress disorder rat model.
Materials and Methods: A total of eighteen female Wistar rats used in the study were exposed to predatory odor stress. One week later, saline was administered to the control group and 15 µM and 30 µM topiramate to the treatment groups, after which the animals were exposed to the trauma reminder and their behavior was monitored in the elevated plus maze. At the end of the experiment, blood samples were taken, animals were sacrificed, salivary glands were removed immediately after.
Results: Topiramate suppressed anxiety index and freezing time in rats with post-traumatic stress disorder at both 15 µM and 30 µM doses compared to the control group. A positive correlation was observed between plasma estrogen level and anxiety index in the control group, and topiramate suppressed this correlation in a dose-dependent manner. Topiramate did not change the plasma progesterone level, but suppressed the salivary gland immunoglobulin A level at the low dose.
Conclusion: These findings obtained in our study indicate that topiramate may be effective in the treatment of post-traumatic stress disorder.

Keywords

Anxiety index, estrogen, freezing time, progesterone, salivary immunoglobulin A

Supporting Institution

Tekirdag Namik Kemal University Scientific Research Projects Coordination Unit

Project Number

NKUBAP.02.DPÖ.21.303

Akut topiramat uygulamasının sıçanlardaki travma sonrası stres bozukluğu üzerine etkileri

Abstract

Amaç: Bu çalışmada, travma sonrası stres bozukluğu sıçan modelinde akut sistemik topiramat uygulamasının anksiyete indeksi ve donakalma zamanı, plazma östrojen ve progesteron düzeyleri ile tükürük bezi immünoglobulin A üzerine etkilerinin araştırılması amaçlanmıştırı.
Gereç ve Yöntem: Çalışmada kullanılan toplam on sekiz dişi Wistar sıçan yırtıcı koku stresine maruz bırakıldı. Bir hafta sonra kontrol grubuna serum fizyolojik, tedavi gruplarına 15 µM ve 30 µM topiramat uygulandı, ardından hayvanlar travma hatırlatıcısına maruz bırakıldı ve davranışları yükseltilmiş artı labirentinde izlendi. Deneyin sonunda kan örnekleri alındı, sakrifiye edilen hayvanların tükürük bezleri çıkarıldı.
Bulgular: Topiramat travma sonrası stres bozukluğu olan sıçanlarda hem 15 µM hem de 30 µM dozlarda kontrol grubuna kıyasla kaygı indeksini ve donakalma zamanını baskıladı. Kontrol grubunda plazma östrojen düzeyi ile kaygı indeksi arasında pozitif korelasyon gözlendi, topiramat bu korelasyonu doza bağımlı olarak baskıladı. Topiramat plazma progesteron seviyesini değiştirmedi, fakat tükürük bezi immünoglobulin A seviyesini düşük dozda baskıladı.
Sonuç: Çalışmamızda elde edilen bu bulgular topiramatın travma sonrası stres bozukluğu tedavisinde etkili olabileceğine işaret etmektedir.

Keywords

Kaygı indeksi, östrojen, donakalma zamanı, progesteron, tükürük immünoglobulin A

Project Number

NKUBAP.02.DPÖ.21.303

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