Anti-inflammatory and anti-apoptotic effects of naringin on bacterial endotoxin-induced small intestine damage in rats

Ali Tuğrul Akin; Mohamed Lemine El Bechir; Emin Kaymak; Tayfun Ceylan; Meryem Sayan; Necla Değer; Derya Karabulut; Ayşe Toluk

doi:10.17826/cumj.1124641

TR EN

Naringinin ratlarda bakteriyel endotoksin kaynaklı ince bağırsak hasarı üzerindeki anti-inflamatuvar ve anti-apoptotik etkileri

Öz

Amaç. Bu çalışmanın amacı, birçok biyolojik özelliği bulunan naringinin (NRG) ratlarda bakteriyel endotoksin kaynaklı ince bağırsak hasarı üzerine anti-inflamatuar ve antiapoptotik etkilerinin araştırılmasıdır. Gereç ve Yöntem: Bu amaçla, 40 adet dişi Wistar albino ırkı rat 4 gruba ayrılmıştır: Kontrol (hiçbir uygulama yapılmayan grup), LPS (10 mg/kg/ip lipopolisakkarit uygulanan grup), NRG (14 gün boyunca 100 mg/kg/ip naringin uygulanan grup) ve LPS+NRG (10 mg/kg/ip lipopolisakkarit uygulamasından önce 14 gün boyunca naringin uygulanan grup). Deneysel prosedürün uygulanmasından sonra, deney hayvanlarının ince barsak dokuları çıkarıldı ve doku takibi protokolüne göre hazırlandı. Barsak dokusundaki histopatolojik değişiklikleri değerlendirmek amacıyla Hematoksilen-Eozin boyaması gerçekleştirildi ve histopatolojik değişiklikler açısından deney gruplarının karşılaştırılması amacıyla hasar skorlaması yapıldı. Ayrıca, immunohistokimyasal boyamalar ile TNF- ve Kaspaz-3 ekspresyon seviyeleri belirlendi ve gruplar arasında bu proteinlerin ekspresyon seviyelerindeki değişikliklerin belirlenmesi için immunohistokimyasal boyanma yoğunluğu skorlandı. Bulgular: LPS grubunda epitel ve Brunner bezlerinde hasar, mononüklear hücre infiltrasyonu, hemorajik alanlar belirlendi. Ayrıca TNF- ve Kaspaz-3 ekspresyonları bu grupta anlamlı bir şekilde arttı. Ancak, NRG uygulamaları bu parametreler açısından LPS+NRG grubundaki deney hayvanlarının ince barsak dokusunda güçlü bir koruyucu etki gösterdi. Sonuç: Bu çalışma, 100 mg/kg NRG enjeksiyonunun endotoksin kaynaklı enfeksiyonun bağırsak mukozası üzerindeki olumsuz etkilerine karşı koruyucu bir ajan olarak kabul edilebileceğini ve daha ileri klinik çalışmalarda göz ardı edilmemesi gerektiğini göstermiştir.

Anahtar Kelimeler

Endotoksin , İnce Bağırsak Hasarı , Lipopolisakkarit , naringin

Anti-inflammatory and anti-apoptotic effects of naringin on bacterial endotoxin-induced small intestine damage in rats

Abstract

Purpose: The aim of this study is to investigate the anti-inflammatory and anti-apoptotic effects of naringin (NRG), which has many biological properties, on bacterial endotoxin-induced small intestine damage in rats. Materials and Methods: For this purpose, 40 female Wistar albino rats were divided into 4 groups as Control (group given no treatment), LPS (group given 10 mg/kg/i.p lipopolysaccharide), NRG (group given 100 mg/kg/i.p naringin for 14 days) and LPS + NRG (group given 100 mg/kg/i.p naringin for 14 days before 10 mg/kg/i.p lipopolysaccharide injection). After experimental procedure, small intestine tissues of animals were extracted and prepared according to tissue processing protocol. Hematoxylin and Eosin staining were performed to evaluate the histopathological changes and histological damage scoring was applied to compare experimental groups in terms of histopathological changes. Moreover, TNF- and Caspase-3 expression levels were detected by immunohistochemical staining and the density of immunoreactivity were scored to determine the difference in the expression levels of TNF- and Caspase-3 expressions among groups. Results: Epithelial and Brunner’s gland damage, mononuclear cell infiltration, hemorrhage, and TNF- and Caspase-3 expressions significantly increased in the LPS group. However, NRG administrations exerted a strong protective effect on the small intestine tissues in terms of these parameters in LPS+NRG group. Conclusion: This study demonstrated that 100 mg/kg NRG injection can be regarded as a protective agent against negative effects of endotoxin-induced infection on the intestinal mucosa and that it should not be disregarded in further clinical trials.

Keywords

Endotoxin , Small intestine damage , lipopolysaccharide , naringin

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Details

Primary Language

English

Subjects

Clinical Sciences

Journal Section

Research Article

Authors

Ali Tuğrul Akin ^*
0000-0002-1408-8571
Türkiye

Mohamed Lemine El Bechir
0000-0002-0550-780X
Türkiye

Emin Kaymak
0000-0002-3818-2693
Türkiye

Tayfun Ceylan
0000-0002-0917-0378
Türkiye

Meryem Sayan
0000-0002-9068-1094
Türkiye

Necla Değer
0000-0001-7239-3331
Türkiye

Derya Karabulut
0000-0003-2067-6174
Türkiye

Ayşe Toluk
0000-0003-1787-505X
Türkiye

Publication Date

September 30, 2022

Submission Date

June 3, 2022

Acceptance Date

August 3, 2022

Published in Issue

Year 1970 Volume: 47 Number: 3

DOI

https://doi.org/10.17826/cumj.1124641

IZ

https://izlik.org/JA35CA62XL

MLA

Akin, Ali Tuğrul, et al. “Anti-Inflammatory and Anti-Apoptotic Effects of Naringin on Bacterial Endotoxin-Induced Small Intestine Damage in Rats”. Cukurova Medical Journal, vol. 47, no. 3, Sept. 2022, pp. 1137-46, doi:10.17826/cumj.1124641.