Ovaryum yüzey epiteli primordial folikül ve primer folikül öncüsü yapılara farklılaşıyor mu?

Murat Serkant Ünal; Mücahit Seçme

doi:10.17826/cumj.1134852

EN TR

Does the ovarian surface epithelium differentiate into primordial follicle and primary follicle precursor structures?

Abstract

Purpose: The aim of this study is to investigate the differentiation capacity of ovarian surface epithelial cells both in cell culture conditions and in ovarian tissue sections. Materials and Methods: The ovaries of two prepubertal (4 weeks old) female rats were divided into small pieces and explant cell culture was created. Ovarian surface epithelium proliferating together with ovarian stromal cells in mixed cell culture was isolated and reproduced. In addition, ovarian surface epithelium was examined in histological sections of ovarian tissue and images were taken under the microscope. Results: The morphological appearance of the ovarian surface epithelium was found to be cobblestone. In the count performed under phase contrast microscopy, it was observed that 2x106 and 3x106 cells were grown in the culture dishes, respectively. Primordial follicle-like structures were observed in some areas of the petri dishes. On the histological sections, primordial and primary follicle precursor structures were observed on the basement membrane. Conclusion: Showing oocyte markers (Gdf-9, C-Mos, Zpc, Stella) and germ cell markers (Dazl, Vasa, Blimp1, Fragilis) both in cell cultures and in histological sections can give us valuable information in terms of monitoring the differentiation capacity of these cells.

Keywords

Ovarian surface epithelium , neoogenesis , primordial follicle , ovarian reserve , ovarian cancer

Ovaryum yüzey epiteli primordial folikül ve primer folikül öncüsü yapılara farklılaşıyor mu?

Öz

Amaç: Ovaryum yüzey epiteli hücrelerinin farklılaşma kapasitelerini hem hücre kültürü şartlarında hem de ovaryum doku kesitlerinde araştırmaktır. Gereç ve Yöntem: İki tane puberte öncesi dönemdeki (4 haftalık) dişi sıçanların ovaryumları küçük parçalara ayrılarak eksplant hücre kültürü oluşturuldu. Miks hücre kültüründe overyan stromal hücrelerle birlikte çoğalan ovaryum yüzey epiteli izole edilerek çoğaltıldı. Bununla birlikte ovaryum dokusunun histolojik kesitlerinde ovaryum yüzey epiteli incelenerek mikroskop altında görüntüleri alındı. Bulgular: Ovaryum yüzey epitelinin morfolojik görünümünün parke taşı (cobblestone) şeklinde olduğu görüldü. Faz kontrast mikroskobisi altında yapılan sayımda kültür kaplarında sırasıyla 2x106 ve 3x106 hücrenin ürediği izlendi. Petri kaplarının bazı alanlarında primordial folikül benzeri yapıların oluştuğu görüldü. Histolojik kesitlerde ise bazal membranın üzerinde primordial ve primer folikül öncüsü yapıların olduğu gözlemlendi. Sonuç: Hem hücre kültürlerinde, hem de histolojik kesitlerde oosit belirteçlerini (Gdf-9, C-Mos, Zpc, Stella) ve germ hücre belirteçlerini (Dazl,Vasa,Blimp1,Fragilis) göstermek bu hücrelerin farklılaşma kapasitelerini izlememiz açısından bizlere değerli bilgiler verebilir.

Anahtar Kelimeler

Ovaryum yüzey epiteli , Neoogenez , Primordial folikül , Ovaryum rezervi Ovaryum kanseri

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Details

Primary Language

Turkish

Subjects

Clinical Sciences

Journal Section

Research Article

Authors

Murat Serkant Ünal
0000-0003-1992-7909
Türkiye

Mücahit Seçme ^*
0000-0002-2084-760X
Türkiye

Publication Date

September 30, 2022

Submission Date

June 23, 2022

Acceptance Date

August 25, 2022

Published in Issue

Year 2022 Volume: 47 Number: 3

DOI

https://doi.org/10.17826/cumj.1134852

IZ

https://izlik.org/JA66TC74GY

MLA

Ünal, Murat Serkant, and Mücahit Seçme. “Ovaryum Yüzey Epiteli Primordial Folikül Ve Primer Folikül öncüsü Yapılara Farklılaşıyor Mu?”. Cukurova Medical Journal, vol. 47, no. 3, Sept. 2022, pp. 1256-62, doi:10.17826/cumj.1134852.

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