Endometrial poliplerde CD56 gen ekspresyonu ve uNK hücre oranının moleküler ve immünohistokimyasal olarak değerlendirmesi

Year 2025, Volume: 50 Issue: 4, 964 - 971, 22.12.2025

Tiinçe Aksak , Harika Topal Önal

https://doi.org/10.17826/cumj.1716574

Abstract

Amaç: Bu çalışmada endometriyal poliplerde uterin doğal öldürücü (uNK) hücre yoğunluğu ve CD56 (NCAM1) gen ekspresyon düzeyinin değerlendirilmesi amaçlanmıştır.
Gereç ve Yöntem: 18-40 yaş arası kadınlardan alınan 50 endometriyal polip ve 50 normal endometriyum örneği immünohistokimyasal ve qPCR analizleri ile değerlendirilmiştir. CD56 pozitif uNK hücreleri immünboyama ile sayılmış ve gen ekspresyon düzeyleri 2-ΔΔCt yöntemi ile hesaplanmıştır.
Bulgular: Kontrol grubunda CD56 gen ekspresyon düzeyinin daha yüksek olduğu belirlenmiştir (ortalama Ct değeri: kontrol grubu ≈ 32; polip grubu ≈ 27). İmmünohistokimyasal analizde ise CD56+ hücre yüzdesi kontrol grubunda ortanca %15,2 (dağılım: %5,4–24,8) iken, polip grubunda ortanca %9,8 (dağılım: %2,1–19,7) bulunmuştur.
Sonuç: Endometriyal poliplerde CD56 gen ekspresyonu ve uNK hücre oranındaki azalma gözlenmiştir. Bu veriler lezyonların bağışıklık mikroçevresinde düzenleyici bir bozulma olabileceğini göstermektedir. Bulgular poliplerin doğurganlık ve endometriyal reseptivite üzerindeki etkilerini açıklamada önemlidir.

Keywords

Endometriyal polip , CD56 , uNK hücre , İmmünohistokimya , Gen ekspresyonu

Ethical Statement

Tarih: 09.02.2024 Sayı: 2

Project Number

no project number

Molecular and immunohistochemical evaluation of CD56 gene expression and uNK cell percentage in endometrial polyps

Abstract

Purpose: This study aimed to investigate the the percentage of uterine natural killer (uNK) cells and the expression level of CD56 (NCAM1) in endometrial polyps.
Materials and Methods: Endometrial tissue samples were obtained from women aged 18–40 years, including 50 with polyps and 50 controls. Samples were analyzed by immunohistochemistry and quantitative PCR (qPCR). CD56-positive uNK cells were quantified by immunostaining, and gene expression was assessed using the 2-ΔΔCt method.
Results: CD56 expression was significantly higher in the control group than in the polyp group (mean Ct values: control ≈ 32; polyp ≈ 27). Immunohistochemical analysis showed that the median percentage of CD56+ cells was 15.2% (range 5.4–24.8%) in the control group and 9.8% (range 2.1–19.7%) in the polyp group.
Conclusion: Endometrial polyps were associated with a significant reduction in CD56 gene expression and uNK cell percentage. These findings indicate a disruption of the local immune microenvironment, which may help explain the negative impact of polyps on fertility and endometrial receptivity.

Keywords

Endometrial polyp , CD56 , uNK cells , Immunohistochemistry , Gene expression

Ethical Statement

Date: 09.02.2024 Number: 2

Supporting Institution

no supporting

Project Number

no project number

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