Bone Marrow Micrometastasis Detected by Flow Cytometry is Associated Bone, Bone Marrow, Lung Macrometastasis in Breast Cancer

Mustafa Salih Akın; Berna Bozkurt Duman; Berksoy Sahin

doi:10.17826/cutf.38648

EN TR

Bone Marrow Micrometastasis Detected by Flow Cytometry is Associated Bone, Bone Marrow, Lung Macrometastasis in Breast Cancer

Abstract

Purpose: The aim of the present study was to analyze deposits in bone marrow for determining micrometastases in breast cancer patients by flow cytometry. And its correlation of prognostic factors and macrometastasis at the first diagnosis. Material and Methods: Bone marrow samples were obtained from 52 breast cancer patients and 16 control patients via aspiration from the iliac spine at the time of first diagnosis after the surgery. Epithelial cells were identified with anti-cytokeratin monoclonal antibody, and double-staining with propidium iodide and CD45using flow cytometry. Results: In all, 2 (12.5%) of the 16 control patients and 11 (21%) of the 52 breast cancer patients had cytokeratin-18 positive cells in their bone marrow. A relationship between the presence of occult metastatic cells in bone marrow, and the presence/absence of lymph node metastases, tumor size, stage, menopausal status, hormone receptor status, histological grade, c-erb-B2 expression, tumor subtype, lymphovascular invasion, Ductal carcinoma in situ (DCIS) component, and gender was not observed. Significant positive relationships were observed between bone marrow micrometastasis, and age, and bone, bone marrow, lung, and liver metastases. Conclusion: Bone marrow micrometastasis was associated with age, bone, bone marrow, lung, and liver metastases at the time of diagnosis..

Keywords

Bone marrow , breast cancer , cytokeratin , flow cytometry , metastasis

Göğüs Kanserinde Flow Sitometri ile Tespit Edilenen Kemik İliği Mikrometastazının Kemik, Kemik İliği, ve Akciğer Makrometastazları ile İlişkilidir

Öz

Amaç: Bu çalışmanın amacı, flow sitometri ile göğüs kanseri hastalarında mikrometastazların belirlenmesi için kemik iliği analizi yapılması ve bulguların ilk tanıdaki prognostik faktorler ve makrometastazlar ile korelasyonur. Materyal ve Metod: Kemik iliği örnekleri 52 göğüs kanserli hasta grubu ve 16 kontrol grubunun iliyak omurundan aspirasyonla alındı. Epitel hücreleri flow sitometrisi kullanılarak propidyum iyodür ve CD45 ile birlikte anti-sitokeratin monoklonal antikor ve ikili boyama ile tanımlandı. Bulgular: Toplamda, 52 göğüs kanseri hastasının 11 (% 21)'inde ve 16 kontrol grubunun 2 (%12.5)'sinde kemik iliğinde sitokeratin 18-pozitif hücreleri vardı. Kemik iliğinde gizli metastatik hücrelerin varlığı, ve lenf nodu metastazının varlığı/yokluğu, tümörün boyutu, evresi, menopozal durumu, hormon reseptör durumu, histolojik derecesi, c-erb-B2 ifadesi, tümör altgrubu, lenfovasküler nöbet, Duktal karsinomanın tamamlayıcı bileşeni (DCIS), ve cinsiyet arasında bir ilişki gözlenmedi. Yaş, kemik, kemik iliği, akciğer ve karaciğer ile kemik iliği mikrometastazı arasında anlamlı pozitif bir ilişki olduğu gözlemlendi. Sonuç: Tanı sırasında yaş, kemik, kemik iliği, akciğer ve karaciğer metastazıyla kemik iliği mikrometastazları ilişkiledirildi.

Anahtar Kelimeler

Kemik iliği , göğüs kanseri , sitokeratin , flow sitometri , metastaz

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Yazışma Adresi / Address for Correspondence: Dr. Berna Bozkurt Duman Cukurova University Faculty of Medicine Department of Medical Oncology Tel:+90322 338 60 60 Fax :+90322 338 70 10
E-mail: berboz@hotmail.com geliş tarihi/received :11.11.2013 kabul tarihi/accepted:09.12.2013

Details

Primary Language

English

Subjects

Clinical Sciences

Journal Section

Research Article

Authors

Mustafa Salih Akın This is me

Berna Bozkurt Duman This is me

Berksoy Sahin This is me

Publication Date

July 22, 2014

Submission Date

July 22, 2014

Acceptance Date

-

Published in Issue

Year 2014 Volume: 39 Number: 2

DOI

https://doi.org/10.17826/cutf.38648

IZ

https://izlik.org/JA97DG96LS

MLA

Akın, Mustafa Salih, et al. “Bone Marrow Micrometastasis Detected by Flow Cytometry Is Associated Bone, Bone Marrow, Lung Macrometastasis in Breast Cancer”. Cukurova Medical Journal, vol. 39, no. 2, June 2014, pp. 305-14, doi:10.17826/cutf.38648.

This journal is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Bone Marrow Micrometastasis Detected by Flow Cytometry is Associated Bone, Bone Marrow, Lung Macrometastasis in Breast Cancer

Abstract

Keywords

Göğüs Kanserinde Flow Sitometri ile Tespit Edilenen Kemik İliği Mikrometastazının Kemik, Kemik İliği, ve Akciğer Makrometastazları ile İlişkilidir

Öz

Anahtar Kelimeler

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

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