Bisfenol A'ya maruz kalan sıçanlarda E vitamininin böbrek histomorfolojisi üzerine etkisi

Mustafa Yıldız; Özay Güleş; Murat Boyacıoğlu; Şadiye Kum

doi:10.17826/cumj.733337

EN TR

Effect of vitamin E on kidney histomorphology in rats exposed to bisphenol A

Abstract

Purpose: The aim of this study was to determine the effects of vitamin E on renal histomorphology in rats exposed to bisphenol A (BPA). Materials and Methods: The rats were randomly divided into four groups as sham, vitamin E, BPA and vitamin E+BPA. The applications of vitamin E and BPA were continued for three weeks. Crossman's triple staining method was applied to the sections obtained from kidney tissues for histomorphological examinations at the end of the experiment. Also, superoxide dismutase (SOD) activity and malondialdehyde (MDA) level were measured in kidney tissue. Results: The number of corpusculum renis, diameters of proximal tubule, distal tubule, ascending limb of Henle’s loop and collecting duct as well as SOD activity significantly decreased in BPA group compared to sham group. Also, the number of corpusculum renis, diameters of ascending limb of Henle’s loop and collecting duct as well as SOD activity significantly increased in vitamin E+BPA group compared to BPA group. On the other hand, MDA level significantly increased in BPA group compared to sham group and it significantly decreased in vitamin E+BPA group compared to BPA group. Conclusion: BPA may cause histomorphological and oxidative changes in the kidney. In addition, vitamin E may contribute to the preservation of renal tissue by reducing oxidative damage in BPA exposure.

Keywords

bisphenol A , rat , vitamin E

Bisfenol A'ya maruz kalan sıçanlarda E vitamininin böbrek histomorfolojisi üzerine etkisi

Öz

Amaç: Sunulan çalışmada, bisfenol A (BPA)'ya maruz bırakılan sıçanlarda E vitamininin böbrek histomofolojisi üzerine etkilerinin belirlenmesi amaçlandı. Gereç ve Yöntem: Sıçanlar sham, E vitamini, BPA ve E vitamini+BPA olmak üzere rastgele dört gruba ayrıldı. E vitamini ve BPA uygulamaları üç hafta boyunca sürdürüldü. Deney sonunda böbrek dokularından elde edilen kesitlere histomorfolojik incelemeler için Crosman’ın üçlü boyama yöntemi uygulandı. Ayrıca böbrek dokusunda süperoksit dismutaz (SOD) aktivitesi ile malondialdehit (MDA) seviyesi ölçüldü. Bulgular: Böbrekte korpuskulum renis sayısı, proksimal tübül, distal tübül, çıkan henle ve toplayıcı kanal çapları ile SOD aktivitesinin sham grubuna göre BPA grubunda anlamlı olarak azaldığı saptandı. Ayrıca korpuskulum renis sayısı, çıkan henle ve toplayıcı kanal çapları ile SOD aktivitesinin BPA grubuna göre E vitamini+BPA grubunda anlamlı olarak arttığı gözlendi. Diğer taraftan, MDA seviyesinin sham grubuna göre BPA grubunda anlamlı olarak arttığı, BPA grubuna göre E vitamini+BPA grubunda ise anlamlı olarak azaldığı tespit edildi. Sonuç: BPA, böbrekte histomorfolojik ve oksidatif değişikliklere neden olabilir. Bununla birlikte, E vitamini BPA maruziyetinde oksidatif hasarı azaltarak böbrek dokusunun korunmasına katkı sağlayabilir.

Anahtar Kelimeler

Bisfenol A , E vitamini , histomorfoloji , böbrek

References

1. Gao H, Yang BJ, Li N, Feng LM, Shi XY, Zhao WH.et al. Bisphenol A and hormone-associated cancers: current progress and perspectives. Medicine (Baltimore). 2015;94:e211.
2. Konieczna A, Rutkowska A, Rachoń D. Health risk of exposure to bisphenol A (BPA). Rocz Panstw Zakl Hig. 2015;66:5-11.
3. Mahmoudi A, Ghorbel H, Bouallegui Z, Marrekchi R, Isoda H, Sayadi S. Oleuropein and hydroxytyrosol protect from bisphenol A effects in livers and kidneys of lactating mother rats and their pups'. Exp Toxicol Pathol. 2015;67:413-25.
4. Gowder SJ. Nephrotoxicity of bisphenol A (BPA)--an updated review. Curr Mol Pharmacol. 2013;6:163-72.
5. Sangai NP, Verma RJ, Trivedi MH. Testing the efficacy of quercetin in mitigating bisphenol A toxicity in liver and kidney of mice. Toxicol Ind Health. 2014;30:581-97.
6. Elshaer FM, Abu-Shaeir WA, Bakry SA. Histopathological changes in the kidney of mosquito fish, Gambusia affinis and guppy fish, Poecilia reticulata exposed to Bisphenol A. Egypt J Aquat Biol & Fish. 2013;17:83-93.
7. Helal EGE, Badawi MMM, Soliman MG, Abdel-Kawi NA, Fadel HAE, Abozaid NMG. Physiological and histopathological studies on bisphenol-A compound as xenoestrogen in male albino rats. The Egyptian Journal of Hospital Medicine. 2013;50:127-36.
8. Ahmed WMS, Moselhy WA, Nabil TM. Bisphenol A toxicity in adult male rats: Hematological, biochemical and histopathological approach. Global Veterinaria. 2015;14:228-38.
9. Faheem M, Jahan N, Lone KP. Histopathological effects of bisphenol-A on liver, kidneys and gills of indian major carp, Catla catla (Hamilton, 1822). The Journal of Animal & Plant Sciences. 2016;26:514-22.
10. Sangai NP, Verma RJ, Trivedi MH. Testing the efficacy of quercetin in mitigating bisphenol A toxicity in liver and kidney of mice. Toxicol Ind Health. 2014;30:581-97.

11. Kobroob A, Peerapanyasut W, Chattipakorn N, Wongmekiat O. Damaging effects of bisphenol A on the kidney and the protection by melatonin: Emerging evidences from in vivo and in vitro studies. Oxid Med Cell Longev. 2018;2018:3082438.
12. Nuñez P, Fernandez T, García-Arévalo M, Alonso-Magdalena P, Nadal A, Perillan C, et al. Effects of bisphenol A treatment during pregnancy on kidney development in mice: a stereological and histopathological study. J Dev Orig Health Dis. 2018;9:208-14.
13. Elobeid, MA, Hassan ZK. Bisphenol-A induced oxidative stress and apoptosis in kidney of male rats. Journal of Environmental Biology. 2015;36:685-8.
14. Rengaraj D, Hong YH. Effects of dietary vitamin E on fertility functions in poultry species. Int J Mol Sci. 2015;16:9910-21.
15. Lee GY, Han SN. The role of vitamin E in immunity. Nutrients. 2018;10:1614.
16. Santana AT, Guelfi M, Medeiros HC, Tavares MA, Bizerra PFV, Mingatto FE. Mechanisms involved in reproductive damage caused by gossypol in rats and protective effects of vitamin E. Biol Res. 2015;48:43.
17. Kedziora-Kornatowska K, Szram S, Kornatowski T, Szadujkis-Szadurski L, Kedziora J, Bartosz G. Effect of vitamin E and vitamin C supplementation on antioxidative state and renal glomerular basement membrane thickness in diabetic kidney. Nephron Exp Nephrol. 2003;95:e134-43.
18. Salehipour M, Monabbati A, Salahi H, Nikeghbalian S, Bahador A, Marvasti VE, et al. Protective effect of parenteral vitamin E on ischemia-reperfusion injury of rabbit kidney. Urology. 2010;75:858-61.
19. Bakar E, Ulucam E, Cerkezkayabekir A. Protective effects of proanthocyanidin and vitamin E against toxic effects of formaldehyde in kidney tissue. Biotechnic & Histochemistry. 2015;90:69-78.
20. Kutlubay R, Oguz EO, Guven C, Can B, Sinik Z, Tuncay OL. Histolocical and ultrasructural evidence for protective effects on aluminium induced kidney damage by intraperitoneal administration of alpha-tocopherol. Int J Toxicol. 2007;26:95-101.
21. Badgujar PC, Pawar NN, Chandratre GA, Telang AG, Sharma AK. Fipronil induced oxidative stress in kidney and brain of mice: protective effect of vitamin E and vitamin C. Pestic Biochem Physiol. 2015;118:10-18.
22. Zhang D, Okada S, Yu Y, Zheng P, Yamaguchi R, Kasai H. Vitamin E inhibits apoptosis, DNA modification, and cancer incidence induced by iron-mediated peroxidation in wistar rat kidney. Cancer Res. 1997;57:2410-14.
23. Abdel-Naim AB, Abdel-Wahab MH, Attia FF. Protective effects of vitamin E and probucol against gentamicin-induced nephrotoxicity in rats. Pharmacol Res. 1999;40:183-7.
24. Dillioglugil MO, Maral Kir H, Gulkac MD, Ozon Kanli A, Ozdogan HK, Acar O, et al. Protective effects of increasing vitamin E and a doses on cisplatin-induced oxidative damage to kidney tissue in rats. Urol Int. 2005;75:340-4.
25. Manali BP, Deshpande S, Shah G. Evaluation of efficacy of vitamin E and N-acetyl cysteine in gentamicin induced nephrotoxicity in rats. Ren Fail. 2011;33:341-7.
26. Aktoz T, Aydogdu N, Alagol B, Yalcin O, Huseyinova G, Atakan IH. The protective effects of melatonin and vitamin E against renal ischemia-reperfusion injury in rats. Ren Fail. 2007;29:535-42.
27. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry. 1979;95:351-8.
28. Sun Y, Oberley LW, Li Y. A simple for clinical assay of superoxide dismutase. Clinical Chemistry. 1988;34:497-500.
29. Klein EA, Thompson IM Jr, Tangen CM, Crowley JJ, Lucia MS, Goodman PJ, et al. Vitamin E and the risk of prostate cancer: the selenium and vitamin E cancer prevention trial (SELECT). JAMA. 2011;306:1549-56.
30. Sesso HD, Buring JE, Christen WG, Kurth T, Belanger C, MacFadyen J, et al. Vitamins E and C in the prevention of cardiovascular disease in men: the physicians' health study II randomized controlled trial. JAMA. 2008;300:2123-33.
31. Schürks M, Glynn RJ, Rist PM, Tzourio C, Kurth T. Effects of vitamin E on stroke subtypes: meta-analysis of randomised controlled trials. BMJ. 2010;341:c5702.
32. El-Hak HNG, ELaraby EE, Hassan AK, Abbas OA. Study of the toxic effect and safety of vitamin E supplement in male albino rats after 30 days of repeated treatment. Heliyon. 2019;5:e02645.

Details

Primary Language

Turkish

Subjects

Biochemistry and Cell Biology (Other) , Anatomy , Clinical Sciences (Other)

Journal Section

Research Article

Authors

Mustafa Yıldız ^*
0000-0003-4128-8947
Türkiye

Özay Güleş
0000-0001-6170-1706
Türkiye

Murat Boyacıoğlu
0000-0001-6952-8637
Türkiye

Şadiye Kum
0000-0001-6586-4596
Türkiye

Publication Date

December 27, 2020

Submission Date

May 9, 2020

Acceptance Date

August 29, 2020

Published in Issue

Year 2020 Volume: 45 Number: 4

DOI

https://doi.org/10.17826/cumj.733337

IZ

https://izlik.org/JA93HP93GB

MLA

Yıldız, Mustafa, et al. “Bisfenol A’ya Maruz Kalan Sıçanlarda E Vitamininin Böbrek Histomorfolojisi üzerine Etkisi”. Cukurova Medical Journal, vol. 45, no. 4, Dec. 2020, pp. 1411-7, doi:10.17826/cumj.733337.

This journal is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Effect of vitamin E on kidney histomorphology in rats exposed to bisphenol A

Abstract

Keywords

Bisfenol A'ya maruz kalan sıçanlarda E vitamininin böbrek histomorfolojisi üzerine etkisi

Öz

Anahtar Kelimeler

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

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