Redox modulatory effects of testosterone administration in liver tissue of aging rats

Karolin Yanar

doi:10.17826/cumj.792233

TR EN

Yaşlanan karaciğer sıçan dokusunda testosteron uygulamasının redoks modulator etkisi

Öz

Amaç: Bu çalışmanın amacı, testosteron uygulamasının erkek sıçanların karaciğer dokusunda yaşlanmaya bağlı bozulan redoks homeostasisi üzerine iyileştirici bir etkisinin olup olmadığını değerlendirmektir. Gereç ve Yöntem: Çalışmamız üç gruptan oluşmaktadır (n=24). Grup 1: Kontrol grubu erkek sıçanlara ve Grup 2: Yaşlı erkek sıçanlara yerfıstığı yağı taşıyıcısı içinde intramusküler olarak verilmiştir. Grup 3’ü oluşturan yaşlı erkek sıçanlara ise tek doz testosteron enanthate (25 mg/kg -) yerfıstığı yağı taşıyıcısı içinde intramuskuler olarak uygulanmıştır. Karaciğer dokusunda makromoleküler hasarı ve antioksidan kapasiteyi belirlemek üzere protein karbonil grupları, ileri oksidasyon protein ürünleri, ditriozin, kinürenin, lipit hidroperoksit, ileri glikasyon son ürünleri, total tiyol fraksiyonları ve Cu, Zn-süperoksit dismutaz aktiviteleri analiz edilmiştir. Bulgular: Çalışma bulgularımız, yaşlı sıçanların karaciğer dokularında redoks dengesinde ileri düzeyde bir bozulmanın olduğunu artan protein, lipit peroksidasyonu biyobelirteçleri ile ve kısmen azalan antioksidan savunma kapasiteleriyle göstermektedir. Bu çalışmada, testosteron uygulamasının bozulmus redoks dengesinde kısmen iyileştirici yönde etki ettiği saptanmıştır. Sonuç: Testosteron uygulamasının makromoleküler oksidatif hasarı hafifletmek ve antioksidan kapasiteyi kısmen arttırarak bozulmuş redoks homeostasisini kısmen iyileştirdiği ancak bu etkinin genç kontrollerinki kadar etkili olmadığı sonucuna varılmıştır.

Anahtar Kelimeler

yaşlanma , karaciğer , oksidatif stres , redoks dengesi , testosteron

Redox modulatory effects of testosterone administration in liver tissue of aging rats

Abstract

Purpose: The aim of this sudy was to evaluate if testosterone administration possesses any ameliorative effects on age-related redox imbalance in liver tissue of male rats. Materials and Methods: Our study consists of three groups of male rats (n=24). Group 1: Young controls and Group 2: Aged rats that were administrated peanut oil intramuscularly andGroup 3: Aged rats that were administered a single dose of testosterone enanthate (25 mg/kg body weight) in peanut oil intramuscularly as a vehicle. To determine oxidative damage and also antioxidative capacity, we analyzed the levels of protein carbonyl groups, advanced oxidation end products, dityrosine, kynurenine, lipid hydroperoxides, advanced glycation end products, total thiol fractions, and Cu, Zn-superoxide dismutase activities. Results: The current study results indicate that redox balance is severely impaired in aged liver tissue via higher levels of oxidative protein damage, increased rate of lipid peroxidation, and also reduced antioxidant defense capacity. the present study showed that, testosterone administrationpartially ameliorated impaired redox balance. Conclusion: Testosterone administration partially improved impaired redox status by alleviating macromolecular oxidative damage and increase antioxidant defense capacity but these ameliorative effects not sufficient as young controls.

Keywords

aging , liver , oxidative stress , redox balance , testosterone

References

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Details

Primary Language

English

Subjects

Biochemistry and Cell Biology (Other)

Journal Section

Research Article

Authors

Karolin Yanar ^*
0000-0001-6571-415X
Türkiye

Publication Date

December 27, 2020

Submission Date

September 8, 2020

Acceptance Date

October 24, 2020

Published in Issue

Year 2020 Volume: 45 Number: 4

DOI

https://doi.org/10.17826/cumj.792233

IZ

https://izlik.org/JA47ZU88UB

MLA

Yanar, Karolin. “Redox Modulatory Effects of Testosterone Administration in Liver Tissue of Aging Rats”. Cukurova Medical Journal, vol. 45, no. 4, Dec. 2020, pp. 1750-7, doi:10.17826/cumj.792233.

This journal is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Yaşlanan karaciğer sıçan dokusunda testosteron uygulamasının redoks modulator etkisi

Öz

Anahtar Kelimeler

Redox modulatory effects of testosterone administration in liver tissue of aging rats

Abstract

Keywords

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

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