Endometriozisli hastaların ektopik ve ötopik dokularında glycodelin A mRNA ekspresyonlarının belirlenmesi

Year 2021, , 310 - 317, 31.03.2021

Khayala Rasulova , Melek Pehlivan , Aygün Akberova , Sefa Kızıldağ

https://doi.org/10.17826/cumj.795826

Abstract

Amaç: Çalışmamızda endometriozisli kadınların sağlıklı bireylere göre sekresyon ve proliferasyon evrelerinde, ektopik ve ötopik endometrium dokularının Glikodelin A mRNA ekspresyon seviyeleri incelenmiştir.
Gereç ve Yöntem: Laparaskopi ve laparatomi sırasında tanı alan ve histopatolojik olarak tanısı doğrulanan endometriozisli 19 hasta ve 7 kontrol grubu çalışmaya dahil edildi (26-45 yaş). Endometriozisli kadınların sekresyon ve proliferasyon evrelerinde alınan ektopik ve ötopik endometrium dokuları ile kontrol grubu endometrium dokularından RNA izolasyonları gerçekleştirildikten sonra cDNA’ya çevrildi. Glikodelin A ekspresyon düzeyleri gerçek zamanlı polimeraz zincir reaksiyonu kullanılarak değerlendirildi.
Bulgular: Sekretuar ve proliferasyon fazda olan endometriozisli hastaların kontrole göre olan ötopik ve ektopik dokularında Glikodelin A ekspresyon düzeylerinin daha yüksek olduğu gözlendi. Ektopik sekretuar fazda olan hastaların Glikodelin A seviyesi, ötopik hastadakilere göre artış gösterdi. Hastalarda proliferasyon fazlarındaki Glikodelin A seviyesi ötopik hastalar ile kıyaslandığında bazı hastalarda ekspresyonun düştüğü saptandı.
Tartışma: Endometriozis’te Glikodelin A geninin sekretuar ve proliferatif fazda ekspresyonsinin yüksek olması ve ektopik sekretuar fazda olan hastaların Glikodelin A seviyesinin, ötopik hastadakilere göre artış göstermesi, Glikodelin A geninin değerlendirilmesinin hastalığın tanısının konulması aşamasında yönlendirici olabileceğini göstermektedir.

Keywords

Endometriosiz, ektopik, ötopik

Determination of glycodelin A mRNA expressions in ectopic and etopic tissues of patients with endometriosis

Abstract

Purpose: In the present study, Glycodelin A mRNA expression levels of ectopic and etopic endometrial tissues were examined in the secretion and proliferation stages of women with endometriosis compared to healthy individuals.
Materials and Methods: 19 Patients with endometriosis, which were diagnosed during laparoscopy and laparotomy and confirmed histopathologically, and 7 control groups were included in the study of people between the age of 26-45. It was converted to cDNA after RNA isolation was performed from ectopic and etopic endometrial tissues taken during the secretion and proliferation stages of women with endometriosis and control group endometrial tissues. Glycodelin A expression levels were evaluated using real-time polymerase chain reaction.
Results: Glycodelin A expression levels were higher in the euphoric and ectopic tissues of the patients with endometriosis in the secretory and proliferating phases. The Glycodelin A level of patients in the ectopic secretory phase increased compared to those in the etopic patient., The expression decreased in some patients when the Glycodelin A level in the proliferation phases of the patients was compared with that of the etopic patient.
Conclusion: High expression of the Glycodelin A gene in the secretory and proliferative phases in endometriosis, and the increase in the Glycodelin A level in patients in the ectopic secretory phase, compared to the etopic patients, indicate that the evaluation of the Glycodelin A gene may be a guide in the diagnosis of the disease.

Keywords

endometriosis, glycodelin A, mRNA

References

• [1] Signorile PG, Baldi A. Endometriosis: new concepts in the pathogenesis. Int J Biochem Cell Biol. 2010 Jun;42(6):778-80.
• [2] Nothnick W, Alali Z. Recent advances in the understanding of endometriosis: the role of inflammatory mediators in disease pathogenesis and treatment. F1000Res. 2016;5:F1000 Faculty Rev-186.
• [3] Sourial S, Tempest N, Hapangama DK. Theories on the pathogenesis of endometriosis. Int J Reprod Med. 2014;2014:179515.
• [4] Dastur AE, Tank PD. John A Sampson and the origins of Endometriosis. J Obstet Gynaecol India. 2010;60(4):299-300.
• [5] Bellelis P, Dias JA Jr, Podgaec S, Gonzales M, Baracat EC, Abrão MS. Epidemiological and clinical aspects of pelvic endometriosis–a case series. Rev Assoc Med Bras (1992). 2010 Jul-Aug;56(4):467-71.
• [6] D. Vinatier, G. Orazi, M. Cosson, and P. Dufour, “Theories of endometriosis,” European Journal of Obstetrics, Gynecology, and Reproductive Biology, 2001. 96(1): 21–34.
• [7] Gupta S, Harlev A, Agarwal A. “Theories on Endometriosis Sampson’s Theory,” Endometriosis. SpringerBriefs in Reproductive Biology. 2015. pp. 17–21.
• [8] M. Hickey, K. Ballard, and C. Farquhar, “Clinical review,” 2014.vol. 1752, no. March, pp. 1–9.
• [9] Missmer SA, Cramer DW. The epidemiology of endometriosis. Obstet Gynecol Clin North Am. 2003;30(1):1-vii.
• [10] Yuka Ozawa, Takashi Murakami, Yukihiro Terada, Nobuo Yaegashi, Kunihiro Okamura, Shinichi Kuriyama et al. Management of the Pain Associated with Endometriosis : An Update of the Painful Problems. TJEM. 2006; pp. 175–188.
• [11] Rahmioglu N, Montgomery GW, Zondervan KT. Genetics of endometriosis. Womens Health (Lond). 2015;11(5):577-586.
• [12] Horowitz, I. R, Cho C, Song M, Flowers L. C, Santanam N, Parthasarathy S, et al. Increased glycodelin levels in gynecological malignancies. International Journal of Gynecological Cancer. 2001; 11(3), 173–179.
• [13] Juliana M, Daniel B.D, Júlio C. R. e S, Rui A.F, Luciana C.V, Cláudia C.P de P et al. “Glycodelin expression in the endometrium of healthy women and in the eutopic and ectopic endometrium of women with endometriosis,” Fertil. Steril. 2009.vol. 91, no. 5, pp. 1676–1680, [14] Mingqing S, Sreemathy R, Sumathi R, Lisa C.F, Ira R.H, John A.R, et al. Angiogenic role for glycodelin in tumorigenesis. Proc Natl Acad Sci U S A. 2001;98(16):9265-9270.
• [15] Seppälä M, Taylor RN, Koistinen H, Koistinen R, Milgrom E. Glycodelin: a major lipocalin protein of the reproductive axis with diverse actions in cell recognition and differentiation. Endocr Rev. 2002;23(4):401-430.
• [16] Marzieh F. S, Tahereh A, Shamila F, Massoud S, Reza Y, Nooshin S et al. Effect of myomectomy on endometrial glutathione peroxidase 3 (GPx3) and glycodelin mRNA expression at the time of the implantation window. Iran Biomed J. 2014;18(2):60-66.
• [17] Seppälä. M, Koistinen H, Koistinen R, Mandelin E, Oehninger S, Clark G.F et al., “Glycodelins : role in regulation of reproduction , potential for contraceptive development and diagnosis of male infertility,” 2017.vol. 13, no. December, pp. 262–269.
• [18] Focarelli R, Luddi A, De Leo V, Capaldo A, Stendardi A, Pavone V et al. Dysregulation of GdA Expression in Endometrium of Women With Endometriosis: Implication for Endometrial Receptivity. Reprod Sci. 2018 Apr;25(4):579-586.
• [19] Mylonas I, Jeschke U, Kunert-Keil C, Shabani N, Dian D, Bauerfeind I, et al. Glycodelin A is expressed differentially in normal human endometrial tissue throughout the menstrual cycle as assessed by immunohistochemistry and in situ hybridization. Fertility and sterility, 2006. 86(5), 1488–1497.
• [20] Hadfield RM, Mardon HJ, Barlow DH, Kennedy SH. Endometriosis in monozygotic twins. Fertility and Sterility. 1997 Nov;68(5):941-942.
• [21] B. M. Analiz, E. Obstetrik, and K. Riskini, “Endometriozis Bülten,” 2017.
• [22] Bulletti C, Coccia ME, Battistoni S, Borini A. Endometriosis and infertility. J Assist Reprod Genet. 2010 Aug;27(8):441-7.
• [23] Ailawadi RK, Jobanputra S, Kataria M, Gurates B, Bulun SE. Treatment of endometriosis and chronic pelvic pain with letrozole and norethindrone acetate: a pilot study. Fertil Steril. 2004 Feb;81(2):290-6.
• [24] Greene R, Stratton P, Cleary SD, Ballweg ML, Sinaii N. Diagnostic experience among 4,334 women reporting surgically diagnosed endometriosis. Fertil Steril. 2009 Jan;91(1):32-9.
• [25] Unutkan, A , Kukulu, K . "Endometriozis İlişkili Ağrının Yönetimi Ve Ağrının Yönetiminde Hemşirenin Rolü" . Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi 3. 2014 : 804-814.
• [26] Harada T. Dysmenorrhea and endometriosis in young women. Yonago Acta Med. 2013 Dec;56(4):81-4.
• [27] Marlon de F.F, Felipe V.S , Lilian de C.A , José A.D de R.Jand Claudio P.C. The Association between Dyspareunia and Dysmenorrhea in Women with Deep Endometriosis : A Pre- Planned Observational Study. 2015vol. 2, pp. 2–5.
• [28] Heidi M. Hausermann, Kathleen M. Donnelly, Stephen C. Bell, Harold G. Verhage, Asgerally T. Fazleabas, Regulation of the Glycosylated β-Lactoglobulin Homolog, Glycodelin [Placental Protein 14:(PP14)] in the Baboon (Papio anubis) Uterus, The Journal of Clinical Endocrinology & Metabolism, Volume 83, Issue 4, 1 April 1998, Pages 1226–1233.
• [29] Saridogan E, Djahanbakhch O, Kervancioglu ME, Kahyaoglu F, Shrimanker K, Grudzinskas JG. Placental protein 14 production by human Fallopian tube epithelial cells in vitro. Hum Reprod. 1997 Jul;12(7):1500-7.
• [30] Kämäräinen M, Leivo I, Koistinen R, Julkunen M, Karvonen U, Rutanen EM, Seppälä M. Normal human ovary and ovarian tumors express glycodelin, a glycoprotein with immunosuppressive and contraceptive properties. Am J Pathol. 1996 May;148(5):1435-43.
• [31] Shabani N, Mylonas I, Kunert-Keil C, Briese V, Janni W, Gerber B et al. Expression of glycodelin in human breast cancer: immunohistochemical analysis in mammary carcinoma in situ, invasive carcinomas and their lymph node metastases. Anticancer Res. 2005 May-Jun;25(3A):1761-4.
• [32] Zhou HM, Ramachandran S, Kim JG, Raynor DB, Rock JA, Parthasarathy S. Implications in the management of pregnancy: II. Low levels of gene expression but enhanced uptake and accumulation of umbilical cord glycodelin. Fertil Steril. 2000 Apr;73(4):843-7.
• [33] Mylonas I, Speer R, Makovitzky J, Richter DU, Briese V, Jeschke U et al. Immunohistochemical analysis of steroid receptors and glycodelin A (PP14) in isolated glandular epithelial cells of normal human endometrium. Histochem Cell Biol. 2000 Nov;114(5):405-11.
• [34] Kocbek V, Vouk K, Mueller MD, Rižner TL, Bersinger NA. Elevated glycodelin-A concentrations in serum and peritoneal fluid of women with ovarian endometriosis. Gynecol Endocrinol. 2013 May;29(5):455-9.
• [35] Wang P, Zhu L, Zhang X. The role of placental protein 14 in the pathogenesis of endometriosis. Reprod Sci. 2013 Dec;20(12):1465-70.
• [36] Vodolazkaia A, El-Aalamat Y, Popovic D, Mihalyi A, Bossuyt X, Kyama CM et al. Evaluation of a panel of 28 biomarkers for the non-invasive diagnosis of endometriosis. Hum Reprod. 2012 Sep;27(9):2698-711.