Yağ doku kaynaklı mezenkimal kök hücrelerin ve koşullu besiyerinin deneysel prematür over yetmezliği modeli üzerine etkileri

Büşra Şen Halıcıoğlu; Mehmet İbrahim Tuğlu

doi:10.17826/cumj.852402

EN TR

Effects of adipose tissue derived mesenchymal stem cells and conditioned medium on experimental premature ovarian insufficiency model

Abstract

Purpose: The aim of this study was to investigate the effects of adipose tissue derived mesenchymal stem cells (ADMSC) and conditioned medium (CM), which can be recommended for the treatment of chemotherapy-induced premature ovarian insufficiency (POI), on oxidative stress and apoptosis. Materials and Methods: In the study, adult female Wistar albino rats were divided into 4 groups; Control, POI, POI+ADMSC, POI+CM. H&E staining was performed for histopathological evaluations in the ovary and all ovarian follicles were counted. iNOS and Caspase3 expressions were shown immunohistochemically as markers of oxidative stress and apoptosis. Results: Follicular cell degenerations and vacuolization were observed in the ovaries of the POI group, while significant decreases were observed in these degenerations in the ADMSC and CM groups. Increased iNOS and Caspase3 expressions in the POI group were statistically significant decreased in both the ADMSC and CM groups. Conclusion: Mesenchymal stem cells and cell-free conditioned medium, which are increasingly used in the field of regenerative medicine, can be an alternative treatment strategy for POI by reducing apoptosis and oxidative stress, which are the main mechanisms of the disease.

Keywords

premature ovarian insufficiency , adipose derived mesenchymal stem cell , conditioned medium , apoptosis , oxidative stress

Yağ doku kaynaklı mezenkimal kök hücrelerin ve koşullu besiyerinin deneysel prematür over yetmezliği modeli üzerine etkileri

Öz

Amaç: Kemoterapi kaynaklı prematür over yetmezliği (POY) tedavisi için önerilebilecek yağ doku mezenkimal kök hücreleri (YDMKH) ve bu hücrelerden elde edilmiş koşullu besiyerinin (KB) oksidatif stres ve apoptoz üzerine etkilerinin araştırılması amaçlandı. Gereç ve Yöntem: Çalışmada Wistar albino cinsi erişkin dişi sıçanlar 4 gruba ayrıldı. Kontrol, POY, POY+YDMKH, POY+KB. Ovaryumda histopatolojik değerlendirmeler için H&E boyamaları yapıldı ve tüm ovaryum folilkülleri sayıldı. Oksidatif stres ve apoptoz belirteci olarak iNOS ve Caspase3 ekspresyonları immunohistokimyasal olarak gösterildi. Bulgular: Analizler sonucu POY grubu ovaryumlarında foliküller hücre dejenerasyonları, vakuolizasyon, gibi dejenerasyonlar gözlenirken, YDMKH ve KB gruplarında bu dejenerasyonlarda anlamlı azalmalar görüldü. POY grubunda artmış olan iNOS ve Caspase3 ekspresyonları YDMKH ve KB gruplarının her ikisinde de istatistiksel olarak anlamlı şekilde azaldı. Sonuç: Rejeneratif tıp alanında her geçen gün kullanımı artan mezenkimal kök hücrelerin ve hücresiz koşullu besiyerinin prematür over yetmezliğinde hastalığın temel mekanizmalarından olan apoptoz ve oksidatif stresin azaltılması yoluyla, POY için alternatif bir tedavi stratejisi olabileceği gösterildi.

Anahtar Kelimeler

prematür over yetmezliği , mezenkimal kök hücre , koşullu besiyeri , apoptoz , oksidatif stres

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Details

Primary Language

Turkish

Subjects

Clinical Sciences , Clinical Sciences (Other)

Journal Section

Research Article

Authors

Büşra Şen Halıcıoğlu ^*
0000-0003-4089-5243
Türkiye

Mehmet İbrahim Tuğlu
0000-0002-0569-8415
Türkiye

Publication Date

June 30, 2021

Submission Date

January 13, 2021

Acceptance Date

February 26, 2021

Published in Issue

Year 2021 Volume: 46 Number: 2

DOI

https://doi.org/10.17826/cumj.852402

IZ

https://izlik.org/JA63XN86LG

MLA

Şen Halıcıoğlu, Büşra, and Mehmet İbrahim Tuğlu. “Yağ Doku Kaynaklı Mezenkimal Kök Hücrelerin Ve Koşullu Besiyerinin Deneysel Prematür over Yetmezliği Modeli üzerine Etkileri”. Cukurova Medical Journal, vol. 46, no. 2, June 2021, pp. 523-31, doi:10.17826/cumj.852402.

This journal is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Effects of adipose tissue derived mesenchymal stem cells and conditioned medium on experimental premature ovarian insufficiency model

Abstract

Keywords

Yağ doku kaynaklı mezenkimal kök hücrelerin ve koşullu besiyerinin deneysel prematür over yetmezliği modeli üzerine etkileri

Öz

Anahtar Kelimeler

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

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