Antioxidant activities of inula viscosa extract and curcumin on U87 cells induced by beta-amyloid

Year 2021, , 583 - 588, 30.06.2021

Ares Alizade , Gülüzar Özbolat

https://doi.org/10.17826/cumj.854213

Cited By: 1

Abstract

Purpose: The aim of this study was to investigate the effects of Inula viscosa extract and Curcumin on the U87 (human astrocytoma cell line) treated with amyloid-beta (Aβ), which is the Alzheimer’s disease (AD) model cell line.
Materials and Methods: Firstly, the cytotoxic potential of ınula and curcumin was investigated in the U87 cells by the colorimetric MTT (3-4,5-dimethyl-thiazolyl-2,5-diphenyltetrazolium bromide) assay. Then, the amount of Total Glutathione, Malondialdehyde (MDA), Glutathione reductase (GR) activities were investigated. ELISA test was used to examine the expression and activity of cleaved Bax and Bcl-2 proteins in the Inula viscosa and Curcumin treated U87 cell lines.
Results: Inula viscosa and Curcumin treatment reduced cell death caused by amyloid-B in cells. It also reduced the oxidative stress caused by amyloid-B, while reducing the activation of the proapoptotic protein Bax, and Bcl-2.
Conclusion: Our results suggest that inula viscosa may represent a new approach in the treatment of Alzheimer's.

Keywords

ınula viscosa, curcumin, Alzheimer, U87

Supporting Institution

Mustafa Kemal University

Project Number

17.M.003

Thanks

This study was funded by the Mustafa Kemal University Research Projects Unit (Project No.: 17.M.003).

Beta-amiloid ile indüklenen U87 hücrelerinde inula viscosa ekstresi ve kurkuminin antioksidan aktiviteleri

Abstract

Amaç: Bu çalışmada, Inula viscosa ekstresi ve Kurkumin'in Alzheimer hastalığı (AD) model hücre dizisi olan amiloid-beta (Aβ), ile tedavi edilen U87 (insan astrositom hücre dizisi) üzerindeki etkilerini araştırmayı amaçladık.
Gereç ve Yöntem: İlk olarak, U87 hücrelerinde inula ve kurkuminin sitotoksik potansiyeli kolorimetrik MTT (3-4,5-dimetil-tiyazolil-2,5-difeniltetrazolyum bromür) testi ile araştırıldı. Daha sonra Total Glutatyon miktarı, Malondialdehit (MDA), Glutatyon redüktaz (GR) aktiviteleri araştırıldı. Inula viscosa ve Curcumin ile muamele edilmiş U87 hücre hatlarında bölünmüş Bax ve Bcl-2 proteinlerinin ekspresyonunu ve aktivitesini incelemek için ELISA testi kullanıldı.
Bulgular: Inula viscosa ve Kurkumin muamelesinin, hücrelerde amiloid-B'nin neden olduğu hücre ölümünü azalttı. Ayrıca, proapoptotik protein Bax ve Bcl-2'nin aktivasyonunu azaltırken amiloid-B'nin neden olduğu oksidatif stresi de azalttı.
Sonuç: Sonuçlarımız, inula viscosa'nın Alzheimer tedavisinde yeni bir yaklaşımı temsil edebileceğini göstermektedir.

Keywords

ınula viscosa, curcumin, U87, Alzheimer

Project Number

17.M.003

References

• 1. Lauro L, Rolih C. Observations and research on an extract Inula viscosa Ait. Boll. Soc. Ital. Biol. Sper;1990; 66: 829-834.
• 2. Talib WH, Zarga, MH, Mahasneh AN. Antiproliferative, Antimicrobial and Apoptosis Inducing Effects of Compounds Isolated from Inula viscosa . Molecules, 2012; 17: 3291-3303
• 3. M.P. Mattson. Pathways towards and away from Alzheimer's disease. Nature, 430 2004 pp. 631-639
• 4. A Nordberg. Mechanisms behind the neuroprotective actions of cholinesterase inhibitors in Alzheimer disease Alzheimer Dis Assoc Disord, 2006;20, S12-S18
• 5. Messaoudi, M. Cytotoxic Effect and Chemical Composition of Inula viscosa from Three Different Regions of Morocco. European Journal of Medicinal Plants,2016; 16(4): 1-9.
• 6. Butterfield, D.A. Sultana, R. Methionine-35 of Aβ (1–42): importance for oxidative stress in Alzheimer disease. Journal of amino acids, 2011; 1-10.
• 7. Gella, A. Durany, N. Oxidative stress in Alzheimer disease. Cell Adh Migr., 2009; 3(1): 88-93.
• 8. Talib, W. H., Abu Zarga, M. H. & Mahasneh, A. M. Antiproliferative, antimicrobial and apoptosis inducing effects of compounds isolated from Inula viscosa. Molecules,2012; 17, 3291–3303.
• 9. Abuhamdah S. Abuhamdah R. Shakya A, et al. Neuropharmacological evaluation of selected Jordanian traditional herbal medicines. Afr. J. Pharm. Pharmacol.2014; 8(48): 1235-1241.
• 10. Cafarchia, C.De Laurentis N. Milillo MA. Losacco V. Puccini V. Antifungal activity of essential oils from leaves and flowers of Inula viscosa by Apulian Region. Parassitologia Dec; 2012; 44: 153-156.
• 11. Messaoudi M. Chahmi N. Mzibri ME, et al. Cytotoxic Effect and Chemical Composition of Inula viscosa from Three Different Regions of Morocco. European Journal of Medicinal Plants 2016;16(4): 1-9.
• 12. Mishra S. Palanivelu K . The effect of curcumin (turmeric) on Alzheimer's disease: An overview .Ann Indian Acad Neurol. 2008; 11(1): 13–19.
• 13. Khurana S. Jain S. Mediratta PK, et al. Protective role of curcumin on colchicine-induced cognitive dysfunction and oxidative stress in rats. Human & experimental toxicology 2012;31(7):686-97.
• 14. Khuwaja G. Khan MM. Ishrat T, et al. Neuroprotective effects of curcumin on 6-hydroxydopamineinduced Parkinsonism in rats: Behavioral, neurochemical and immunohistochemical studies. Brain Res 2012 ; 12;1368:254-63.
• 15. Lim GP. Chu T. Yang FS, et al. The curry spice curcumin reduces oxidative damage and amyloid pathology in an Alzheimer transgenic mouse. J Neurosci 2001; 1;21(21):8370-7.
• 16. Brullo S, Marco G. Taxonomical revision of the genus Dittrichia (Asteraceae). Porttugaliae Actua Biol. 2000;19:341-54
• 17. 17.Ferlay J, Colombet M, Soerjomataram I, Mathers C, Parkin DM, PiñerosM, et al. Estimating the global cancer incidence and mortality in 2018; Int J Cancer. 2018; 144:1941–53
• 18. Santhi VS. Salame L. Ethanolic extracts of Inula viscosa, Salix alba and Quercus calliprinos, negatively affect the development of the entomopathogenic nematode, Heterorhabditis bacteriophora – A model to compare gastro-intestinal nematodes developmental effect. Journal of Invertebrate Pathology 2017;145;39-44.
• 19. Yao Y. Huang JZ. Chen Y. Tang X. Lı X. Effects and mechanism of amyloid β1 42 on mitochondria in astrocytes. Molecular medıcıne reports, 2018; 17: 6997-7004.
• 20. A.M. Seca, A. Grigore, D.C. Pinto, A.M. Silva. The genus Inula and their metabolites: from ethnopharmacological to medicinal uses. J Ethnopharmacol, 154 (2014), pp. 286-310.
• 21. Çelik TA. Aslantürk AS. Evaluation of Cytotoxicity and Genotoxicity of Inula viscosa Leaf Extracts with Allium Test. Journal of Biomedicine and Biotechnology,2010; doi:10.1155/2010/189252.
• 22. Picciano AP. Vaden TD. Complexation between Cu(II) and curcumin in the presence of two different segments of amyloid β. Biophysical Chemistry.2013; 184, 62--67.
• 23. Lin C.W., Yang L.Y., Shen S.C., Chen Y.C. IGF-I plus E2 induces proliferation via activation of ROS-dependent ERKs and JNKs in human breast carcinoma cells. J Cell Physiol, 2007; 212(3): 666-74.
• 24. Zhu X., Lee H.G., Moreira P.I., Smith M.A., Perry G. Presenilin mutation: A deadly first hit in Alzheimer disease - A commentary on "aging sensitizes towards ROS formation and lipid peroxidation in PS1M146L transgenic mice". Free Rad Bio and Med, 2006; 40(5): 737-739.
• 25. Oh P.S., Lim K.T. Blocking of intracellular ROS production by phytoglycoprotein (30 kDa) causes anti-proliferation.
• 26. Halliwell B, Gutteridge JM: Oxygen free radicals and iron in relation to biology and medicine: some problems and concepts. Archives of biochemistry and biophysics 1986, 246(2):501-514
• 27. Lovell MA, Ehmann WD, Butler SM, Markesbery WR: Elevated Thiobarbituric Acid-Reactive Substances and Antioxidant Enzyme-Activity in the Brain in Alzheimers-Disease. Neurology 1995, 45(8):1594-1601.
• 28. Gsell W, Conrad R, Hickethier M, Sofic E, Frolich L, Wichart I, Jellinger K, Moll G, Rnsmayr G, Beckmann H et al: Decreased Catalase Activity but Unchanged Sueroxide-Dismutase Activity in Brains.
• 29. Xue X. Wang J. Sİ G. Wang C. Zhou S. Synthesis, DNA-binding properties and cytotoxicity evaluation of two copper(II) complexes based on curcumin. Transition Met Chem, 2016; 41: 331--337.
• 30. Gella, A, Durany, N. Oxidative stress in Alzheimer disease. Cell Adh Migr 2009; 3(1): 88-93.
• 31. Delobette, S., Privat, A., Maurice, T., In vitro aggregation facilitates β-amyloid peptide-(25--35)-induced amnesia in the rat. European Journal of Pharmacology, 1997; 319, 1--4.
• 32. Oh P.S., Lim K.T. Blocking of intracellular ROS production by phytoglycoprotein (30 kDa) causes anti-proliferation in bisphenol A-stimulated Chang liver cells. J Appl Toxicol, 2008; 28(6): 749-58.
• 33. Park MY, Jeong YJ, Kang GC, et al: Nitric oxide-induced apoptosis of human dental pulp cells is mediated by the mitochondria-dependent pathway. Korean J Physiol Pharmacol 18: 25-32, 2014
• 34. Tomatır A G. Apoptosis: programmed cell death. Turkiye Klinikleri J Med Sci 2003;23:499-508.
• 35. Cline AM, Radic MZ. Apoptosis, subcellular particles, and autoimmunity. Clin Immunol. 2004;112(2):175-82.