Gene expression analysis of FABP4 in gastric cancer

Year 2016, Volume: 41 Issue: 2, 248 - 252, 30.06.2016

Abdulkarim Karim

https://doi.org/10.17826/cutf.202020

Cited By: 1

Abstract

Purpose: Gastric cancer has high incidence and mortality rate in several countries and is still one of the most frequent and lethal disease. In this study, we aimed to determine diagnostic markers in gastric cancer by molecular techniques; include mRNA expression analysis of FABP4 gene. Fatty acid binding protein 4 (FABP4) gene encodes the fatty acid binding protein found in adipocytes. The protein encoded by FABP4 are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. 
Material and Methods: Total RNA were extracted from paired tumor and normal tissues of 47 gastric cancer. The mRNA expression level of FABP4 was measured employing semi- quantitative reverse transcription- polymerase chain reaction (RT- PCR). 
Results: The mRNA expression level of FABP4 was significantly decreased (down- regulated). 
Conclusion: Down-regulation of FABP4 gene seems to occur at the initial steps of gastric cancer development. In order to confirm the relationship between the gastric tumor and FABP4 gene, further analysis like immunohistochemistry and epigenetc techniques are necessary.

Keywords

-

Gastrik kanserde FABP4 gen ekspresyon analizi

Abstract

Amaç: Gastrik kanser birçok ülkede yüksek oran ve mortalite oranına sahiptir ve günümüzde halen en sık görülen ölümcül hastalıklarından biridir. Bu çalışmada, gastrik kanseri teşhisini için moleküler tekniklerle FABP4 geninin mRNA ekspresyon analizini içeren yeni markırlar belirlemeyi amaçladık. Yağ asidi bağlayıcı protein 4 (FABP4) geni adipositlerde bulunan yağ asidi bağlayıcı proteini kodlar. FABP4 tarafından kodlanan protein, uzun zincirli yağ asitleri ve diğer hidrofobik ligandlara bağlanan küçük, yüksek düzeyde korunmuş, sitoplazmik bir protein ailesidir. FABP’ün yağ asidi alımı, taşınması ve metabolizmayı içeren görevleri olduğu düşünülmektedir.
Gereç ve Yöntem: Total RNA, 47 gastrik kanserinin normal dokularından ve eşleştirilmiş tümörden çıkarılmıştır. FABP4 mRNA ekspresyon seviyesi yarı-kantitatif reverse transkripsiyon polimeraz zincir reaksiyonu (RT-PCR) kullanılarak ölçülmüştür.
Bulgular: FABP4’ün mRNA ekspresyon seviyesi önemli ölçüde azalmıştır (down regüle olmuştur).
Sonuç: FABP4 geninin down-regülasyonunun, gastrik kanserinin başlangıç aşamalarında meydana geldiği görünmektedir. İmmünohistokimya ve epigenetik teknikler gibi ileri analizler, FABP4 geni ve gastrik kanser arasındaki ilişkiyi doğrulamak için gereklidir.

Keywords

Gastrik kanser, FABP4, ekspresyon analizi, yarı-kantitatif RT-PCR

References

• Kamangar A. Patterns of cancer incidence, mortality, and prevalence across five continents: defining priorities to reduce cancer disparities in different geographic regions of the world. J Clin Oncol. 2006;24:2137-50.
• Siegel RL, Naishadham D, Jemal A. Cancer statistics. CA Cancer J Clin. 2012;62:10-29.
• Crew KD. Epidemiology of gastric cancer. World J Gastroenterol. 2006;12:354-62.
• Bryan J, Bigam A. Gastric adenocarcinoma review and considerations for future directions. Ann Surg. 2005:241:27–39.
• Dou, K.F. Down-regulated full-length novel gene GDDR and its effect on gastric cancer. Zhonghua Yi Xue Za Zhi. 2003;10:1166-8.
• Roder DM. The epidemiology of gastric cancer. Gastric Cancer. 2002;5(Suppl 1):5–11. 7. FAB4 gene (protein coding) http://www.genecards.org/cgi-bin/carddisp.pl? gene= FABP4.
• Yong J, Wei D. Screening of key genes in gastric cancer with DNA microarray analysis. Eur J Med Res. 2013;18:2-7.
• Glenn E. Simmons Jr., Wendy M. Pruitt and Kevin Pruitt. Diverse roles of SIRT1 in cancer biology and lipid metabolism. Int J Mol Sci. 2015;16:950-65.
• Amer A, Igci M, Ersin B, Khailany R.A, Bayraktar E, Arslan A. Down-regulated gene expression of GKN1 and GKN2 as diagnostic markers for gastric cancer. 18th International Conference on Cellular and Molecular Biology. Istanbul, Turkey, May 21-22 2015.
• Khailany RA, Igci M, Bayraktar E, Erturhan S, Karakok M, Arslan A. VHL, PBRM1 and SETD2 genes in kidney cancer: a molecular investigation. International Journal of Medical, Health, Biomedical and Pharmaceutical Engineering. 2015;9:389-92.
• Junker K, Ficarra V, Kwon ED, Leibovich BC, Thompson R, Oosterwijk E. Potential role of genetic markers in the management of kidney cancer,” Eur Urol. 2013;63:333-40.
• Jung H, 1 Cao Zhang, Meishan J , Young H, Suk W, Park J. Inactivation of the gastrokine 1 gene in gastric adenomas and carcinomas. Cancer Res Treat. 2004;36:103-14.
• Goel A, Boland C. Epigenetics of colorectal cancer. Gastroenterology. 2012;143:1442-60.