Gene expression of P53 and adipoq as diagnostic markers for colorectal cancer

Year 2016, Volume: 41 Issue: 2, 217 - 223, 30.06.2016

Nadhum Ismaiel Rozhgar Mohammed Hazha Hidayat

https://doi.org/10.17826/cutf.197918

Cited By: 1

Abstract

Purpose: Colorectal cancer is the most frequent cause of death and had high mortality rate in Western world. It is from complex, variable and patient- specific interaction between genetic, epigenetic and environmental factors. In the present study, we aimed to investigate the contribution of gene expression of the P53 and Adipoq, both genes to the risk of colorectal cancer. P53 gene is a tumor suppressor gene, encoded protein of P53 is a transcription factor and its pivotal role in maintaining genomic stability. Adipoq gene codes adiponectin.
Material and Methods: Total RNA were extracted from paired tumor and normal tissues of 32 colorectal cancer patients. The mRNA expression level of P53 and Adipoq were measured employing semi- quantitative reverse transcription- polymerase chain reaction (RT- PCR). 
Results: The mRNA expression level of P53 in colorectal cancer was significantly increased according to normal samples (over-expressed). However, the mRNA expression level of Adipoq in colorectal cancer was significantly reduced according to normal samples (down- regulated).
Conclusion: In current study, our data suggest those reduced mRNA expression of the Adipoq and increased mRNA expression of P53 might be useful molecular diagnostic markers for colorectal cancer patients. In order to understand the investigation between colorectal cancer and diagnostic biomarker; further analysis is necessary.

Keywords

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Kolorektal kanser tanı belirteçleri olarak adipoq ve P53 gen ekspresyonları

Abstract

Amaç: Kolorektal kanser Batı dünyasında çok sık karşılaşılan ve ölüm oranı yüksek bir hastalıktır. Genetik, epigenetik ve çevresel faktörler arasında, hasta spesifik bir interaksiyon vardır, dolayısı ile çok çeşitli ve komplekstir. Bu çalışmada P53 ve Adipoq genlerinin ekspresyon seviyelerinin, kolorektal kanser riski üzerine etkisini incelemeyi amaçladık. P53 geni tümör süpressör bir gen olup, bir transkripsiyon faktörü olan P53 proteinini kodlar, genomik stabilitenin devamlılığında çok önemlidir. Adipoq geni adiponektini kodlar.
Gereç ve Yönetim: Otuz iki kolorektal kanserli hastanın, tümör ve normal dokusundan RNA izolasyonu yapıldı. P53 ve Adipoq mRNA ekspresyon seviyeleri semi-quantitative reverse transkripsiyon-polimeraz zincir reaksiyonu (RT-PCR) yöntemi ile belirlendi.
Bulgular: Kolorektal kanserde, P53 ekspresyon seviyesinin normal dokuya göre önemli oranda artış gösterdiği tesbit edilmiştir (aşırı ekspresyon). Fakat, Adipoq ekspresyon seviyesinin, kolorektal kanserinde normal dokuya oranla önemli derecede azaldığı belirlenmiştir (azalmış ekspresyon).
Sonuç: Yaptığımız bu çalışmada elde ettiğimiz veriler, Adipoq gen ekspresyonu seviyesinin azaldığı, P53 geni ekspresyon seviyesinin ise artış gösterdiğine dair olup, bu genlerin kolorektal kanserli hastalarda teşhis markırı olarak kullanılabileceğini önermektedir. Kolorektal kanser ve teşhis biyomarkırları arasındaki ilişkiyi anlamak için, daha fazla çalışma yapılması gerekir.

Keywords

Kolorektal kanser, P53, adipoq

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