Association of P53 gene expression alteration among breast cancer patients in Erbil province

Year 2017, Volume: 42 Issue: 2, 205 - 209, 30.06.2017

Rozhgar A. Khailany , Mustafa S. Al-attar Amer A. Hasan

https://doi.org/10.17826/cutf.322799

Abstract

Purpose: Breast cancer is the most lethal disease and the leading cause of cancer death among women in worldwide. P53, a tumor-suppressor gene, is best known to be associated with human cancers and more than 50% of human cancers contain P53 alteration, which is guardian of the genome. In the current study, we aimed to evaluate mRNA expression level of P53 gene among breast cancer patients in Erbil province.

Material and Methods: Thirty four pairs breast cancer tissues and their corresponding non-cancerous breast tissues that were grouped according to the types of breast cancer and clinical features of patients were examined by semi- quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) technique.

Results: Expression quantity of P53 gene in breast cancer samples was significantly increased (Up-regulated) according to expression quantity of normal samples.

Conclusion: The over-expression of p53 gene might be a potential molecular genetics marker for breast cancer diagnosis in women; further analysis are mandatory to a better understand and confirm our preliminary findings.

Keywords

Breast cancer, P53, expression analysis, semi-quantitative RT-PCR

Erbil’de meme kanseri hastalarında P53 geni ekspresyonundaki değişikliklerin ilişkisi

Abstract

Amaç: Meme kanseri, dünyadaki kadınlarda ölümcül en büyük hastalık ve kanser ölümünün önde gelen nedenidir. P53, tümör baskılayıcı bir gen, insan kanserleri ile ilişkili olduğu bilinen ve insan kanserlerinin% 50'sinden fazlası,esas görevi genomun koruyucusu olan P53 geninin alterasyonunu içerir. Bu çalışmada, Erbil ilindeki meme kanseri hastalarında P53 geninin mRNA ekspresyon düzeyini değerlendirmeyi amaçladık.

Gereç ve Yöntem: Otuz dört hastaya ait meme kanseri dokusu ve yine bu hastalara ait kanserli olmayan göğüs dokuları meme kanseri tiplerine ve hastaların klinik özelliklerine göre gruplandırıldı ve yarı kantitatif ters transkriptaz polimeraz zincir reaksiyonu (qRT-PCR) tekniği ile incelendi.

Bulgular: Normal örneklerin ekspresyon miktarına göre meme kanseri örneklerinde P53 geninin ekspresyon miktarı önemli ölçüde arttmıştır.(upregüle).

Sonuç: P53 geninin aşırı ekspresyonu kadınlarda meme kanseri tanısı için potansiyel bir moleküler genetik belirteç olabilir; ön bulgularımızı daha iyi anlamak ve doğrulamak için daha fazla analiz yapılması zorunludur.

Keywords

Meme kanseri, P53, ekspresyon analizi, yarı kantitatif RT-PCR

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