An Efficient Method of Extracting PCR Amplifiable DNA from Human Skeletal Fragments and Teeth Specimens.

Year 2014, Volume: 39 Issue: 4, 0 - , 22.07.2014

Abdullahi Daudu Zagga Somaia Muhammad Ismail Aziz Abdo Tadros Hamidu Ahmed

https://doi.org/10.17826/cutf.81784

Abstract

Purpose:Extraction and successful PCR amplification of DNA from humanremains in historical and forensic cases have great importance. The purpose of this study is to demonstrate the efficiency of a method of DNA extraction and PCR amplification of embalmed dried human cadaveric skeletal fragments and teeth specimens from Sokoto, Northwestern Nigeria. Materials and Methods: The efficiency of a method of DNA extraction and PCR amplification was tested on thirteen (13) embalmed dried human cadaveric skeletal fragments and nine (9) teeth specimens from Sokoto, Northwestern Nigeria were used in the study. Results: Of the 13 embalmed dried human cadaveric skeletal fragments, 12 (92.3%) samples amplified with apparent bands. For the 9 embalmed dried human teeth specimens, 7 (78%) samples amplified with apparent bands. Conclusion: The study has shown that our method of DNA extraction and PCR amplification was efficient on embalmed dried human cadaveric skeletal fragments and teeth specimens, from Sokoto, Northwestern Nigeria.

Keywords

Extraction, PCR amplifiable DNA, embalmed, dried human cadaveric bones and teeth.

Diş Örneklerinden ve İnsan İskelet Parçalarından DNA"nın PCR ile Çoğaltılmasında Etkili Bir Yöntem

Abstract

Amaç:Tarihsel ve adl ivakalard ainsandan DNA"nın izolasyonu ve başarılı PCR amplifikasyonu büyük öneme sahiptir. Bu çalışmanın amacı; Kuzeybatı Nijerya, Sokoto"dan diş örnekleri ve mumyalanmış kurutulmuş insane kadavra iskelet parçalarının DNA ayıklanması ve PCR amplifikasyonu için etkili bir yöntemi göstermektir. Materyal ve Metod: Çalışmaya dahil edilen Kuzeybatı Nijerya, Sokoto"dan 9 diş örneğinin ve 13 mumyalanmış kurutulmuş insane kadavra iskelet parçalarının DNA izolasyonu ve PCR amplifikasyonu için etkil ibir metot ile test edildi. Bulgular: 13 mumyalanmış kurutulmuş insane kadavra iskelet parçalarının olduğu örneklerin 12"sinde bantlar çok iyi gözlendi. 9 diş örneğin 7"sinde ise yine bantlar barizdi. Sonuç: Bu çalışma, Kuzeybatı Nijerya,Sokoto"dan mumyalanmış kurutulmuş insane kadavra iskelet parçalarının ve diş örneklerinin DNA izolasyonuve PCR amplifikasyonu için kullandığımız yöntemin verimli olduğunu gösterdi.

Keywords

İzolasyon, kurutulmuş insan kadavra kemikleri ve dişleri, mumyalamak, PCR

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• YazışmaAdresi / Address for Correspondence: Dr. Abdullahi Daudu. Zagga, Department of Anatomy, College of Health Sciences, Usmanu Danfodiyo University, P. M. B. 2346, Sokoto, Sokoto State, Nigeria.
• E-mail: [e-mail] G eliş tarihi/Received on :06.01.2014
• Kabul tarihi/Accepted on: 14.02.2014