Bone Marrow Micrometastasis Detected by Flow Cytometry is Associated Bone, Bone Marrow, Lung Macrometastasis in Breast Cancer

Year 2014, Volume: 39 Issue: 2, 305 - 314, 22.07.2014

Mustafa Salih Akın Berna Bozkurt Duman Berksoy Sahin

https://doi.org/10.17826/cutf.38648

Abstract

Purpose: The aim of the present study was to analyze deposits in bone marrow for determining micrometastases in breast cancer patients by flow cytometry. And its correlation of prognostic factors and macrometastasis at the first diagnosis. Material and Methods: Bone marrow samples were obtained from 52 breast cancer patients and 16 control patients via aspiration from the iliac spine at the time of first diagnosis after the surgery. Epithelial cells were identified with anti-cytokeratin monoclonal antibody, and double-staining with propidium iodide and CD45using flow cytometry. Results: In all, 2 (12.5%) of the 16 control patients and 11 (21%) of the 52 breast cancer patients had cytokeratin-18 positive cells in their bone marrow. A relationship between the presence of occult metastatic cells in bone marrow, and the presence/absence of lymph node metastases, tumor size, stage, menopausal status, hormone receptor status, histological grade, c-erb-B2 expression, tumor subtype, lymphovascular invasion, Ductal carcinoma in situ (DCIS) component, and gender was not observed. Significant positive relationships were observed between bone marrow micrometastasis, and age, and bone, bone marrow, lung, and liver metastases. Conclusion: Bone marrow micrometastasis was associated with age, bone, bone marrow, lung, and liver metastases at the time of diagnosis..

Keywords

Bone marrow, breast cancer, cytokeratin, flow cytometry, metastasis

Göğüs Kanserinde Flow Sitometri ile Tespit Edilenen Kemik İliği Mikrometastazının Kemik, Kemik İliği, ve Akciğer Makrometastazları ile İlişkilidir

Abstract

Amaç: Bu çalışmanın amacı, flow sitometri ile göğüs kanseri hastalarında mikrometastazların belirlenmesi için kemik iliği analizi yapılması ve bulguların ilk tanıdaki prognostik faktorler ve makrometastazlar ile korelasyonur. Materyal ve Metod: Kemik iliği örnekleri 52 göğüs kanserli hasta grubu ve 16 kontrol grubunun iliyak omurundan aspirasyonla alındı. Epitel hücreleri flow sitometrisi kullanılarak propidyum iyodür ve CD45 ile birlikte anti-sitokeratin monoklonal antikor ve ikili boyama ile tanımlandı. Bulgular: Toplamda, 52 göğüs kanseri hastasının 11 (% 21)'inde ve 16 kontrol grubunun 2 (%12.5)'sinde kemik iliğinde sitokeratin 18-pozitif hücreleri vardı. Kemik iliğinde gizli metastatik hücrelerin varlığı, ve lenf nodu metastazının varlığı/yokluğu, tümörün boyutu, evresi, menopozal durumu, hormon reseptör durumu, histolojik derecesi, c-erb-B2 ifadesi, tümör altgrubu, lenfovasküler nöbet, Duktal karsinomanın tamamlayıcı bileşeni (DCIS), ve cinsiyet arasında bir ilişki gözlenmedi. Yaş, kemik, kemik iliği, akciğer ve karaciğer ile kemik iliği mikrometastazı arasında anlamlı pozitif bir ilişki olduğu gözlemlendi. Sonuç: Tanı sırasında yaş, kemik, kemik iliği, akciğer ve karaciğer metastazıyla kemik iliği mikrometastazları ilişkiledirildi.

Keywords

Kemik iliği, göğüs kanseri, sitokeratin, flow sitometri, metastaz

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• Yazışma Adresi / Address for Correspondence: Dr. Berna Bozkurt Duman Cukurova University Faculty of Medicine Department of Medical Oncology Tel:[phone] Fax :[phone]
• E-mail: [e-mail] geliş tarihi/received :11.11.2013 kabul tarihi/accepted:09.12.2013