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Evaluation of Mean Platelet Volume Before and After Cobalamin Treatment in Patients with Vitamin B12 Deficiency

Year 2014, Volume: 39 Issue: 2, 329 - 335, 22.07.2014
https://doi.org/10.17826/cutf.96679

Abstract

Purpose: Megaloblastic anemia due to vitamin B12 deficiency is common in the population. Vitamin B12 therapy may stimulate all cell types in the bone marrow. We investigated whether young, active and large platelets are released into the peripheral blood during vitamin B12 treatment and measured the level of mean platelet volume (MPV), an indicator of the presence of these platelets. Materials and Methods: A total of 204 patients (40 males, 160 females) with vitamin B12 deficiency were included in this study. Cobalamin was administered by intramuscular injections. We evaluated the hematologic and biochemical parameters before and after one month of vitamin B12 treatment. Results: The mean age of patients was 40.1 ± 17.4 years. In the pretreatment group, the mean level of vitamin B12 was 151.2 ± 34.6 pg/mL, the MPV was 7.8 ± 1.4 fL. In the posttreatment group, the mean vitamin B12 level was 638 ± 608 pg/mL, the MPV was 8.3 ± 1.3 fL. The levels of vitamin B12 (P < 0.001) and MPV (P < 0.001) were significantly higher in the posttreatment group than those in the pretreatment group. Conclusions: After one month of cobalamin treatment in patients with vitamin B12 deficiency, the levels of B12 and MPV were higher than the pretreatment levels. These results showed that vitamin B12 treatment may increases the release of large and active thrombocytes into the peripheral blood; therefore, caution may be needed in patients predisposed to thrombotic diseases.

References

  • Moreno-Garcia MA, Rosenblatt DS, JeromeMajewska LA. Vitamin B12 metabolism during pregnancy and in embryonic mouse models. Nutrients. 2013;5:3531-50.
  • Lachner C, Steinle NI, Regenold WT. The neuropsychiatry of vitamin B12 deficiency in elderly patients. J Neuropsychiatry Clin Neurosci 2012;24:5
  • Healton EB, Savage DG, Brust JC, Garrett TJ, Lindenbaum J. Neurologic aspects of cobalamin deficiency. Medicine (Baltimore). 1991;70:229-45.
  • Pawlak R, Parrott SJ, Raj S, Cullum-Dugan D, Lucus D. How prevalent is vitamin B(12) deficiency among vegetarians? Nutr Rev. 2013;71:110-7.
  • Stabler SP. Clinical practice. Vitamin B12 deficiency. N Engl J Med. 2013;368:149-60.
  • Green R. Anemias beyond B12 and iron deficiency: the buzz about other B's, elementary, and nonelementary problems. Hematology Am Soc Hematol Educ Program. 2012;2012:492-8.
  • Langan RC, Zawistoski KJ. Update on vitamin B12 deficiency. Am Fam Physician. 2011;83:1425-30. den Elzen WP, van der Weele GM, Gussekloo J, Westendorp RG, Assendelft WJ. Subnormal vitamin
  • B12 concentrations and anaemia in older people: a systematic review. BMC Geriatr. 2010;10:42.
  • Demirin H, Ozhan H, Ucgun T, Celer A, Bulur S, Cil H, et al. Normal range of mean platelet volume in healthy subjects: Insight from a large epidemiologic study. Thromb Res. 2011;128:358-60.
  • Cure E, Balik MS, Cumhur Cure M, Guvercin Y, Erkut A, Yuce S, et al. Is the mean platelet volume predictive of hip fractures in the elderly? Ann Lab Med. 2013;33:367-70.
  • Cure MC, Cure E, Kirbas A, Cicek AC, Yuce S. The effects of Gilbert's syndrome on the mean platelet volume and other hematological parameters. Blood Coagul Fibrinolysis. 2013;24:484-8.
  • Akgul O, Uyarel H, Pusuroglu H, Gul M, Isiksacan N, Turen S, et al. Prognostic value of elevated mean platelet volume in patients undergoing primary angioplasty for ST-elevation myocardial infarction. Acta Cardiol. 2013;68:307-14.
  • Beyan C. Is mean platelet volume a predictive marker in patients with venous thrombosis? Clin Appl Thromb Hemost. 2012;18:670-1.
  • Silva D, Albers U, Santana I, Vicente M, Martins IP, Verdelho A, et al. Do MCI patients with vitamin B12 deficiency have distinctive cognitive deficits? BMC Res Notes. 2013;6:357.
  • Watkins D, Whitehead M, Rosenblatt DS. Megaloblastic anemia. In: Orkin S, Nathan DG, Ginsburg D, Look AT, Fisher DE, Lux SE. Hematology of infancy and childhood. 7th ed. Saunders Elsevier, Philadelphia. 2009;467-520.
  • Kocer A, Ince N, Canbulat CE, Sargin M. Serum vitamin B12 and folic Acid levels in acute cerebral atherothrombotic infarction. Tohoku J Exp Med. 2004;204:155-61.
  • Siri PW, Verhoef P, Kok FJ. Vitamins B6, B12, and folate: association with plasma total homocysteine and risk of coronary atherosclerosis. J Am Coll Nutr.1998;17:435-41.
  • Oh R, Brown DL. Vitamin B12 deficiency. Am Fam Physician. 2003;67:979-86.
  • Marniemi J, Jarvisalo J, Toikka T, Raiha I, Ahotupa M, Sourander L. Blood vitamins, mineral elements and inflammation markers as risk factors of vascular and non-vascular disease mortality in an elderly population. Int J Epidemiol. 1998;27:799-807.
  • Wu K, Helzlsouer KJ, Comstock GW, Hoffman SC, Nadeau MR, Selhub J. A prospective study on folate, B12, and pyridoxal 5'-phosphate (B6) and breast cancer. Cancer Epidemiol Biomarkers Prev. 1999;8:209-17.
  • Stanger O, Fowler B, Piertzik K, Huemer M, Haschke-Becher E, Semmler A, et al. Homocysteine, folate and vitamin B12 in neuropsychiatric diseases: review and treatment recommendations. Expert Rev Neurother. 2009;9:1393-412.
  • Blundo C, Marin D, Ricci M. Vitamin B12 deficiency associated with symptoms of frontotemporal dementia. Neurol Sci. 2011;32:101-5.
  • Ouzzif Z, Oumghar K, Sbai K, Mounach A, Derouiche el M, El Maghraoui A. Relation of plasma total homocysteine, folate and vitamin B12 levels to bone mineral density in Moroccan healthy postmenopausal women. Rheumatol Int. 2012;32:123-8.
  • Funada U, Wada M, Kawata T, Mori K, Tamai H, Isshiki T, et al. Vitamin B-12-deficiency affects immunoglobulin production and cytokine levels in mice. Int J Vitam Nutr Res. 2001;71:60-5.
  • Fora MA, Mohammad MA. High frequency of suboptimal serum vitamin B12 level in adults in Jordan. Saudi Med J. 2005;26:1591-5.
  • Stabler SP, Allen RH, Savage DG, Lindenbaum J. Clinical spectrum and diagnosis of cobalamin deficiency. Blood. 1990;76:871-81.
  • Kaptan K, Beyan C, Ural AU, Cetin T, Avcu F, Gulsen M, et al. Helicobacter pylori is it a novel causative agent in Vitamin B12 deficiency? Arch Intern Med. 2000;160:1349-53.
  • Bernard M, Babior H, Franklin B. Megaloblastic anemias. In : Harrison's Principles of Internal Medicine. Kasper DL, Braunwald E, Fauci AS, Hauser SL, Longo DL, Jameson JL, editors. 16th ed. McGraw-Hill: New York. 2005;601-7.
  • Stabler SP, Allen RH. Megaloblastic anemias. In: Cecil Textbook of Medicine. 22nd ed. Philadelphia, Pa: WB Saunders. 2004.
  • Bolaman Z, Kadikoylu G, Yukselen V, Yavasoglu I, Barutca S, Senturk T. Oral versus intramuscular cobalamin treatment in megaloblastic anemia: a single-center, prospective, randomized, open-label study. Clin Ther. 2003;25:3124-34.
  • Lance MD, Sloep M, Henskens YM, Marcus MA. Mean platelet volume as a diagnostic marker for cardiovascular disease: drawbacks of preanalytical conditions and measuring techniques. Clin Appl Thromb Hemost. 2012;18:561-8.
  • Arikanoglu A, Yucel Y, Acar A, Cevik MU, Akil E, Varol S. The relationship of the mean platelet volume and C-reactive protein levels with mortality in ischemic stroke patients. Eur Rev Med Pharmacol Sci. 2013;17:1774-7.
  • Chandra H, Chandra S, Rawat A, Verma SK. Megaloblastic pancytopenia vis-a-vis nonmegaloblastic pancytopenia: is mean platelet volume useful discriminating indicator. Int J Lab Hematol. 2011;33:409-13.
  • Yazışma Adresi / Address for Correspondence: Dr. Medine Cumhur Cure University of Recep Tayyip Erdogan, School of Medicine, Department of Biochemistry Islampasa mahallesi, 53100, R İZE e mail: medinecure@yahoo.com geliş tarihi/received :13.01.2014 kabul tarihi/accepted:31.01.2014

B12 Vitamin Eksikliği Olan Hastalarda Kobalamin Tedavisi Öncesi ve Sonrası Ortalama Trombosit Volümünün Değerlendirilmesi

Year 2014, Volume: 39 Issue: 2, 329 - 335, 22.07.2014
https://doi.org/10.17826/cutf.96679

Abstract

Amaç: B12 vitamin eksikliği toplumda sık görülen bir megaloblastik anemidir. B12 tedavisi kemik iliğindeki tüm serileri stimüle edebilir. Biz bu çalışmada B12 tedavisi sırasında periferik kana genç, aktif ve büyük trombositlerin salınıp salınmadığını ve bu trombositlerin varlığının iyi bir göstergesi olan ortalama trombosit hacmi (OTH) seviyesini araştırmayı amaçladık. Materyal ve Metod: Bu çalışmaya 40 erkek ve 160 kadın toplam 204 hasta dahil edildi. Kobalamin intramuskuler (İM) enjeksiyon yoluyla uygulandı. B12 tedavisinden önce ve tedaviden bir ay sonra hematolojik ve biyokimyasal testleri değerlendirdik. Bulgular: Hastaların yaş ortalaması 40.1 ± 17.4 yıl idi. Tedavi öncesi grupta, B12 vitamin seviyeleri 151.2 ± 34.6 pg/mL ve OTH 7.8 ± 1.4 fL idi. Tedavi sonrası grupta, B12 vitamin seviyeleri 638 ± 608 pg/mL ve OTH 8.3 ± 1.3 idi. Tedavi sonrası grupta B12 vitamin (P < 0.001) ve OTH (P < 0.001) tedavi öncesi gruptan anlamlı yüksekti. Sonuç: B12 eksikliği olan hastalarda İM kobalamin tedavisinin 1. ayı sonunda tedavi öncesi değerlere göre B12 ve OTH değerinin arttığı MCV değerinin düştüğü bulundu. Çalışmamızın sonuçları gösterdi ki İM B12 tedavisi sırasında büyük ve aktif trombositler perifere daha çok salınabilir; trombotik hastalıklara yatkın olan hastalarda dikkatli olmak gerekebilir.

References

  • Moreno-Garcia MA, Rosenblatt DS, JeromeMajewska LA. Vitamin B12 metabolism during pregnancy and in embryonic mouse models. Nutrients. 2013;5:3531-50.
  • Lachner C, Steinle NI, Regenold WT. The neuropsychiatry of vitamin B12 deficiency in elderly patients. J Neuropsychiatry Clin Neurosci 2012;24:5
  • Healton EB, Savage DG, Brust JC, Garrett TJ, Lindenbaum J. Neurologic aspects of cobalamin deficiency. Medicine (Baltimore). 1991;70:229-45.
  • Pawlak R, Parrott SJ, Raj S, Cullum-Dugan D, Lucus D. How prevalent is vitamin B(12) deficiency among vegetarians? Nutr Rev. 2013;71:110-7.
  • Stabler SP. Clinical practice. Vitamin B12 deficiency. N Engl J Med. 2013;368:149-60.
  • Green R. Anemias beyond B12 and iron deficiency: the buzz about other B's, elementary, and nonelementary problems. Hematology Am Soc Hematol Educ Program. 2012;2012:492-8.
  • Langan RC, Zawistoski KJ. Update on vitamin B12 deficiency. Am Fam Physician. 2011;83:1425-30. den Elzen WP, van der Weele GM, Gussekloo J, Westendorp RG, Assendelft WJ. Subnormal vitamin
  • B12 concentrations and anaemia in older people: a systematic review. BMC Geriatr. 2010;10:42.
  • Demirin H, Ozhan H, Ucgun T, Celer A, Bulur S, Cil H, et al. Normal range of mean platelet volume in healthy subjects: Insight from a large epidemiologic study. Thromb Res. 2011;128:358-60.
  • Cure E, Balik MS, Cumhur Cure M, Guvercin Y, Erkut A, Yuce S, et al. Is the mean platelet volume predictive of hip fractures in the elderly? Ann Lab Med. 2013;33:367-70.
  • Cure MC, Cure E, Kirbas A, Cicek AC, Yuce S. The effects of Gilbert's syndrome on the mean platelet volume and other hematological parameters. Blood Coagul Fibrinolysis. 2013;24:484-8.
  • Akgul O, Uyarel H, Pusuroglu H, Gul M, Isiksacan N, Turen S, et al. Prognostic value of elevated mean platelet volume in patients undergoing primary angioplasty for ST-elevation myocardial infarction. Acta Cardiol. 2013;68:307-14.
  • Beyan C. Is mean platelet volume a predictive marker in patients with venous thrombosis? Clin Appl Thromb Hemost. 2012;18:670-1.
  • Silva D, Albers U, Santana I, Vicente M, Martins IP, Verdelho A, et al. Do MCI patients with vitamin B12 deficiency have distinctive cognitive deficits? BMC Res Notes. 2013;6:357.
  • Watkins D, Whitehead M, Rosenblatt DS. Megaloblastic anemia. In: Orkin S, Nathan DG, Ginsburg D, Look AT, Fisher DE, Lux SE. Hematology of infancy and childhood. 7th ed. Saunders Elsevier, Philadelphia. 2009;467-520.
  • Kocer A, Ince N, Canbulat CE, Sargin M. Serum vitamin B12 and folic Acid levels in acute cerebral atherothrombotic infarction. Tohoku J Exp Med. 2004;204:155-61.
  • Siri PW, Verhoef P, Kok FJ. Vitamins B6, B12, and folate: association with plasma total homocysteine and risk of coronary atherosclerosis. J Am Coll Nutr.1998;17:435-41.
  • Oh R, Brown DL. Vitamin B12 deficiency. Am Fam Physician. 2003;67:979-86.
  • Marniemi J, Jarvisalo J, Toikka T, Raiha I, Ahotupa M, Sourander L. Blood vitamins, mineral elements and inflammation markers as risk factors of vascular and non-vascular disease mortality in an elderly population. Int J Epidemiol. 1998;27:799-807.
  • Wu K, Helzlsouer KJ, Comstock GW, Hoffman SC, Nadeau MR, Selhub J. A prospective study on folate, B12, and pyridoxal 5'-phosphate (B6) and breast cancer. Cancer Epidemiol Biomarkers Prev. 1999;8:209-17.
  • Stanger O, Fowler B, Piertzik K, Huemer M, Haschke-Becher E, Semmler A, et al. Homocysteine, folate and vitamin B12 in neuropsychiatric diseases: review and treatment recommendations. Expert Rev Neurother. 2009;9:1393-412.
  • Blundo C, Marin D, Ricci M. Vitamin B12 deficiency associated with symptoms of frontotemporal dementia. Neurol Sci. 2011;32:101-5.
  • Ouzzif Z, Oumghar K, Sbai K, Mounach A, Derouiche el M, El Maghraoui A. Relation of plasma total homocysteine, folate and vitamin B12 levels to bone mineral density in Moroccan healthy postmenopausal women. Rheumatol Int. 2012;32:123-8.
  • Funada U, Wada M, Kawata T, Mori K, Tamai H, Isshiki T, et al. Vitamin B-12-deficiency affects immunoglobulin production and cytokine levels in mice. Int J Vitam Nutr Res. 2001;71:60-5.
  • Fora MA, Mohammad MA. High frequency of suboptimal serum vitamin B12 level in adults in Jordan. Saudi Med J. 2005;26:1591-5.
  • Stabler SP, Allen RH, Savage DG, Lindenbaum J. Clinical spectrum and diagnosis of cobalamin deficiency. Blood. 1990;76:871-81.
  • Kaptan K, Beyan C, Ural AU, Cetin T, Avcu F, Gulsen M, et al. Helicobacter pylori is it a novel causative agent in Vitamin B12 deficiency? Arch Intern Med. 2000;160:1349-53.
  • Bernard M, Babior H, Franklin B. Megaloblastic anemias. In : Harrison's Principles of Internal Medicine. Kasper DL, Braunwald E, Fauci AS, Hauser SL, Longo DL, Jameson JL, editors. 16th ed. McGraw-Hill: New York. 2005;601-7.
  • Stabler SP, Allen RH. Megaloblastic anemias. In: Cecil Textbook of Medicine. 22nd ed. Philadelphia, Pa: WB Saunders. 2004.
  • Bolaman Z, Kadikoylu G, Yukselen V, Yavasoglu I, Barutca S, Senturk T. Oral versus intramuscular cobalamin treatment in megaloblastic anemia: a single-center, prospective, randomized, open-label study. Clin Ther. 2003;25:3124-34.
  • Lance MD, Sloep M, Henskens YM, Marcus MA. Mean platelet volume as a diagnostic marker for cardiovascular disease: drawbacks of preanalytical conditions and measuring techniques. Clin Appl Thromb Hemost. 2012;18:561-8.
  • Arikanoglu A, Yucel Y, Acar A, Cevik MU, Akil E, Varol S. The relationship of the mean platelet volume and C-reactive protein levels with mortality in ischemic stroke patients. Eur Rev Med Pharmacol Sci. 2013;17:1774-7.
  • Chandra H, Chandra S, Rawat A, Verma SK. Megaloblastic pancytopenia vis-a-vis nonmegaloblastic pancytopenia: is mean platelet volume useful discriminating indicator. Int J Lab Hematol. 2011;33:409-13.
  • Yazışma Adresi / Address for Correspondence: Dr. Medine Cumhur Cure University of Recep Tayyip Erdogan, School of Medicine, Department of Biochemistry Islampasa mahallesi, 53100, R İZE e mail: medinecure@yahoo.com geliş tarihi/received :13.01.2014 kabul tarihi/accepted:31.01.2014
There are 34 citations in total.

Details

Primary Language Turkish
Journal Section Research
Authors

Süleyman Yüce This is me

Medine Cumhur Cüre This is me

Erkan Cüre This is me

Sefa Kıztanır This is me

Abdulkadir Baştürk This is me

Hasan Efe This is me

Publication Date July 22, 2014
Published in Issue Year 2014 Volume: 39 Issue: 2

Cite

MLA Yüce, Süleyman et al. “B12 Vitamin Eksikliği Olan Hastalarda Kobalamin Tedavisi Öncesi Ve Sonrası Ortalama Trombosit Volümünün Değerlendirilmesi”. Cukurova Medical Journal, vol. 39, no. 2, 2014, pp. 329-35, doi:10.17826/cutf.96679.